FastQCFastQC Report
Fri 10 Feb 2017
ERR1630475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317696
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT134051.0173059643498956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133821.0155604934673854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT102630.7788594637913449No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78710.5973304920102968No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA31710.24064731167128078No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC27540.20900116567098936No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC25460.19321603769002865No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG23610.17917638059157803No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC22860.17348462771382778No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA22720.1724221671766477No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG22390.1699177959104376No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21390.16232879207343728No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19760.1499587158191267No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT19520.14813735489824661No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC19380.1470748943610666No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC19270.14624010393899656No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.14578476370877652No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG18150.13774041964155617No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC18140.13766452960318618No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT18130.13758863956481615No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG17780.13493248822186604No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC17680.13417358783816602No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG17360.1317451066103259No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA17200.13053086599640584No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC16850.12787471465345573No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG16320.12385254261984555No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG16180.1227900820826655No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG15990.12134817135363543No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC15510.11770544951187527No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC15480.11747777939676526No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.11618764874447521No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC15110.11466984797707513No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT14880.11292437709456507No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG14800.11231725678760504No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT14610.11087534605857498No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG14360.10897809509932488No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC14350.10890220506095487No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG14330.10875042498421487No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC14220.10791563456214483No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT14180.10761207440866483No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC14140.1073085142551848No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14080.10685317402496478No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC13740.10427291272038466No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG13460.10214799164602459No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG13410.10176854145417455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC652.6833422E-622.769234
AACTGTA957.1468094E-921.4210532
AAGGGCC705.10286E-621.14285730
GCAGTCG3500.020.6142859
GACCATG450.003826100620.5555577
ACACTGC450.003826100620.5555576
CCTGGAC450.003826100620.5555573
CTGGGGA450.003826100620.55555712
TCAAGGG450.003826100620.5555573
TAAGATA450.003826100620.5555574
ACTGTAT1001.2902092E-820.3499983
CTACGTA555.1436445E-420.1818189
ACGTACA555.1436445E-420.18181811
TACGTAC555.1436445E-420.18181810
GCCGTCT1303.274181E-1119.92307736
GGCAGTC3750.019.7333328
GGGCAGT4050.019.1851847
CAGTCGG3700.019.010
CACATGT3600.018.528
GCAGCGT902.152703E-618.51
Produced by FastQC (version 0.11.5)