##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630475.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1317696
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.897785984020594	33.0	31.0	34.0	30.0	34.0
2	32.03639610350187	33.0	31.0	34.0	30.0	34.0
3	32.10850757686143	33.0	31.0	34.0	30.0	34.0
4	35.71378451478945	37.0	35.0	37.0	33.0	37.0
5	35.71840849482734	37.0	35.0	37.0	35.0	37.0
6	35.79079544902618	37.0	35.0	37.0	35.0	37.0
7	35.757442536062946	37.0	35.0	37.0	35.0	37.0
8	35.77773932682501	37.0	35.0	37.0	35.0	37.0
9	37.44569384744281	39.0	37.0	39.0	34.0	39.0
10	37.34889762130264	39.0	37.0	39.0	34.0	39.0
11	37.4772610678032	39.0	37.0	39.0	35.0	39.0
12	37.4165907766283	39.0	37.0	39.0	34.0	39.0
13	37.47080130773714	39.0	37.0	39.0	35.0	39.0
14	38.648508457185876	40.0	38.0	41.0	34.0	41.0
15	38.61140581742678	40.0	38.0	41.0	34.0	41.0
16	38.55629978386517	40.0	38.0	41.0	34.0	41.0
17	38.602122189032976	40.0	38.0	41.0	34.0	41.0
18	38.60224285419399	40.0	38.0	41.0	34.0	41.0
19	38.65821327529263	40.0	38.0	41.0	34.0	41.0
20	38.62189078512798	40.0	38.0	41.0	34.0	41.0
21	38.539492417067365	40.0	38.0	41.0	34.0	41.0
22	38.4552013514498	40.0	38.0	41.0	34.0	41.0
23	38.39430187235903	40.0	38.0	41.0	34.0	41.0
24	38.3272264619457	40.0	38.0	41.0	34.0	41.0
25	38.24739697168391	40.0	38.0	41.0	34.0	41.0
26	37.99085525037641	40.0	37.0	41.0	33.0	41.0
27	37.81517208825101	40.0	37.0	41.0	33.0	41.0
28	37.6668419726553	40.0	37.0	41.0	33.0	41.0
29	37.53968441886444	40.0	36.0	41.0	32.0	41.0
30	37.365012112050124	39.0	36.0	41.0	32.0	41.0
31	37.259757941133614	39.0	36.0	41.0	32.0	41.0
32	37.09114773058429	39.0	35.0	41.0	31.0	41.0
33	36.98880925494196	39.0	35.0	41.0	31.0	41.0
34	36.82932785710816	39.0	35.0	41.0	31.0	41.0
35	36.66422376633154	39.0	35.0	41.0	30.0	41.0
36	36.55952586939628	39.0	35.0	41.0	30.0	41.0
37	36.267013028801784	39.0	35.0	41.0	30.0	41.0
38	36.05747380275876	39.0	35.0	41.0	29.0	41.0
39	35.8754006994026	39.0	35.0	41.0	28.0	41.0
40	35.64529451406091	39.0	35.0	41.0	26.0	41.0
41	35.41463888484142	38.0	35.0	40.0	25.0	41.0
42	35.16034578537083	38.0	35.0	40.0	24.0	41.0
43	34.283812806595755	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	4.0
12	7.0
13	8.0
14	4.0
15	7.0
16	15.0
17	32.0
18	81.0
19	162.0
20	352.0
21	686.0
22	1205.0
23	2005.0
24	3290.0
25	4984.0
26	7421.0
27	10715.0
28	15080.0
29	20172.0
30	26033.0
31	32530.0
32	40322.0
33	50680.0
34	65190.0
35	83601.0
36	114750.0
37	180578.0
38	282683.0
39	375089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24525839040264	20.74795704016708	12.766601704793823	22.240182864636456
2	19.82521006362621	21.253005245519454	34.006933313905485	24.914851376948857
3	21.592157826995	25.543069114575744	28.605687503035604	24.259085555393657
4	13.826026640439068	16.32516149400165	34.04859694497062	35.800214920588665
5	12.94919313711205	39.93182038952839	32.924817256787605	14.194169216571956
6	32.12531570255962	34.458479042207	16.69596022147749	16.72024503375589
7	27.93686859488076	31.972928505512655	21.726103744718053	18.36409915488853
8	29.021944363495074	31.08592573704405	19.48749939287969	20.404630506581185
9	27.798596944970615	12.70194339210258	22.027463087085337	37.47199657584147
10	20.308022487736167	27.291955048812472	30.454292947690515	21.945729515760842
11	33.5046930399728	23.36555624362524	20.388769488561852	22.74098122784011
12	22.74363737918306	29.02057834280441	26.11232029238914	22.12346398562339
13	29.040006192627132	20.394688911554717	27.72999235028413	22.835312545534023
14	22.241928335518963	22.471343921511487	27.113917018796446	28.172810724173104
15	26.738033658749817	27.071342707270873	25.37193707805139	20.818686555927922
16	24.15762057409296	25.962968696876978	26.953789037835733	22.92562169119433
17	23.301277381125843	27.337564961872847	26.23184710282189	23.129310554179416
18	21.573412987517607	24.29429853319734	30.0702893535383	24.06199912574676
19	22.16429282626645	25.999699475448057	29.976944606343192	21.8590630919423
20	22.991190684346012	24.021170285103697	31.260928165525282	21.726710865025016
21	23.368591845160037	26.049028000388557	29.595597163533927	20.98678299091748
22	24.854442906406334	25.352053887998444	28.1667395211035	21.626763684491717
23	22.60991913157511	24.912195225605906	29.7838803487299	22.694005294089077
24	21.487884914274613	27.119001651367235	28.217130506581185	23.17598292777697
25	21.993160789742095	25.770132109378796	29.8284278012531	22.408279299626013
26	21.959769172859296	27.521598304920104	28.9626742435281	21.555958278692504
27	23.004775001214238	26.516434746709404	28.633159696925542	21.84563055515081
28	21.356139807664288	26.34355723930254	30.206436082374083	22.09386687065909
29	21.86862523677692	26.461642139006265	30.389331074845792	21.28040154937102
30	19.599968429744038	27.465363786487927	30.95319405993492	21.981473723833115
31	22.058729782893778	27.696221283209482	28.360942129292336	21.8841068046044
32	20.201093423672837	26.671098644907477	29.492690271504202	23.63511765991549
33	19.969704696682694	26.826673223565983	29.770599592015152	23.433022487736167
34	21.87613835057555	25.929956530186022	30.176004176987714	22.01790094225072
35	20.53576849288455	27.584511146728836	29.805888459857204	22.07383190052941
36	21.03019209286512	27.321628053815143	28.805885424255674	22.842294429064065
37	21.018960367186363	25.46748263635922	30.246202462479964	23.26735453397445
38	20.74363130797999	24.98444254213415	29.972618874156105	24.29930727572976
39	20.646719728981495	26.08841493030259	30.59901525086211	22.665850089853805
40	19.64815860410899	26.37748008645393	30.238234008451116	23.736127300985963
41	20.10061501287095	25.626548156782746	30.60463111370149	23.66820571664481
42	19.361294259070377	26.97420345815727	30.947350526980426	22.717151755791928
43	19.252012603817573	24.656749356452476	30.540883481470686	25.550354558259265
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1889.0
1	1215.5
2	542.0
3	1333.5
4	2125.0
5	2125.0
6	3141.0
7	4157.0
8	4250.0
9	4343.0
10	6417.5
11	8492.0
12	8492.0
13	14833.0
14	21174.0
15	27732.0
16	34290.0
17	30803.0
18	27316.0
19	27316.0
20	28355.0
21	29394.0
22	21630.0
23	13866.0
24	12872.0
25	11878.0
26	11878.0
27	13068.0
28	14258.0
29	17210.0
30	20162.0
31	24528.5
32	28895.0
33	28895.0
34	36683.0
35	44471.0
36	49152.0
37	53833.0
38	64165.0
39	74497.0
40	74497.0
41	79224.5
42	83952.0
43	90766.0
44	97580.0
45	95456.0
46	93332.0
47	93332.0
48	97689.5
49	102047.0
50	100043.5
51	98040.0
52	94838.5
53	91637.0
54	91637.0
55	84184.0
56	76731.0
57	67662.5
58	58594.0
59	55440.5
60	52287.0
61	52287.0
62	48718.0
63	45149.0
64	40782.0
65	36415.0
66	34275.5
67	32136.0
68	32136.0
69	25765.0
70	19394.0
71	17347.5
72	15301.0
73	11393.5
74	7486.0
75	7486.0
76	5834.5
77	4183.0
78	3051.5
79	1920.0
80	1676.0
81	1432.0
82	1432.0
83	1432.5
84	1433.0
85	1438.5
86	1444.0
87	1150.5
88	857.0
89	857.0
90	738.5
91	620.0
92	367.0
93	114.0
94	67.5
95	21.0
96	21.0
97	14.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1317696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.174498983017134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.87534657674641	19.35298334215392
2	10.157995704619847	5.114449050708894
3	3.589122221487378	2.710630611442746
4	1.8254861672442364	1.8382279864320747
5	1.1433538738878852	1.439168046770963
6	0.826535309505567	1.2484567385145386
7	0.6080646312863872	1.071540570934949
8	0.4941443181852436	0.9951868508494487
9	0.36388040355165147	0.8244456164235822
>10	3.1601368906064837	16.277797027348033
>50	0.48312282438070575	8.51511204944063
>100	0.39608163471382163	19.65432564790581
>500	0.055196674975374345	9.643085803821084
>1k	0.020319655073352094	7.884925032577391
>5k	3.032784339306282E-4	0.6009416115363511
>10k+	9.098353017918847E-4	2.8287240131396016
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13405	1.0173059643498956	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13382	1.0155604934673854	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10263	0.7788594637913449	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7871	0.5973304920102968	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3171	0.24064731167128078	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	2754	0.20900116567098936	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2546	0.19321603769002865	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	2361	0.17917638059157803	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2286	0.17348462771382778	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	2272	0.1724221671766477	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	2239	0.1699177959104376	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2139	0.16232879207343728	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1976	0.1499587158191267	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1952	0.14813735489824661	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	1938	0.1470748943610666	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1927	0.14624010393899656	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1921	0.14578476370877652	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1815	0.13774041964155617	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1814	0.13766452960318618	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	1813	0.13758863956481615	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1778	0.13493248822186604	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	1768	0.13417358783816602	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	1736	0.1317451066103259	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	1720	0.13053086599640584	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1685	0.12787471465345573	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG	1632	0.12385254261984555	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	1618	0.1227900820826655	No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG	1599	0.12134817135363543	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1551	0.11770544951187527	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	1548	0.11747777939676526	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1531	0.11618764874447521	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1511	0.11466984797707513	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	1488	0.11292437709456507	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	1480	0.11231725678760504	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	1461	0.11087534605857498	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	1436	0.10897809509932488	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	1435	0.10890220506095487	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1433	0.10875042498421487	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1422	0.10791563456214483	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	1418	0.10761207440866483	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	1414	0.1073085142551848	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1408	0.10685317402496478	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1374	0.10427291272038466	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	1346	0.10214799164602459	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1341	0.10176854145417455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.276701151100102E-4	0.0
3	0.0	0.0	0.0	3.79450191850017E-4	0.0
4	0.0	0.0	0.0	9.106804604400408E-4	0.0
5	0.0	0.0	0.0	0.0010624605371800475	0.0
6	0.0	0.0	0.0	0.001138350575550051	0.0
7	0.0	0.0	0.0	0.0013660206906600612	0.0
8	0.0	0.0	0.0	0.0014419107290300646	0.0
9	0.0	0.0	0.0	0.0022008111127300987	0.0
10	0.0	0.0	0.0	0.003642721841760163	0.0
11	0.0	0.0	0.0	0.005084632570790228	0.0
12	0.0	0.0	0.0	0.009106804604400408	0.0
13	0.0	0.0	0.0	0.010169265141580456	0.0
14	0.0	0.0	0.0	0.012066516100830541	0.0
15	0.0	0.0	0.0	0.01722703870999077	0.0
16	0.0	0.0	0.0	0.028003424158531253	0.0
17	0.0	0.0	0.0	0.04515457283015202	0.0
18	0.0	0.0	0.0	0.05152933605323231	0.0
19	0.0	0.0	0.0	0.06147093107970275	0.0
20	0.0	0.0	0.0	0.07019828549225314	0.0
21	0.0	0.0	0.0	0.09197872650444412	0.0
22	0.0	0.0	0.0	0.13637439895089612	0.0
23	0.0	0.0	0.0	0.20649679440477925	0.0
24	0.0	0.0	0.0	0.3138811986983341	0.0
25	0.0	0.0	0.0	0.334295619019865	0.0
26	0.0	0.0	0.0	0.3765663703919569	0.0
27	0.0	0.0	0.0	0.42506010491038904	0.0
28	0.0	0.0	0.0	0.5152174704939531	0.0
29	0.0	0.0	0.0	0.6377039924231386	0.0
30	0.0	0.0	0.0	0.8581645538879984	0.0
31	0.0	0.0	0.0	1.448285492253145	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	65	2.6833422E-6	22.76923	4
AACTGTA	95	7.1468094E-9	21.421053	2
AAGGGCC	70	5.10286E-6	21.142857	30
GCAGTCG	350	0.0	20.614285	9
GACCATG	45	0.0038261006	20.555557	7
ACACTGC	45	0.0038261006	20.555557	6
CCTGGAC	45	0.0038261006	20.555557	3
CTGGGGA	45	0.0038261006	20.555557	12
TCAAGGG	45	0.0038261006	20.555557	3
TAAGATA	45	0.0038261006	20.555557	4
ACTGTAT	100	1.2902092E-8	20.349998	3
CTACGTA	55	5.1436445E-4	20.181818	9
ACGTACA	55	5.1436445E-4	20.181818	11
TACGTAC	55	5.1436445E-4	20.181818	10
GCCGTCT	130	3.274181E-11	19.923077	36
GGCAGTC	375	0.0	19.733332	8
GGGCAGT	405	0.0	19.185184	7
CAGTCGG	370	0.0	19.0	10
CACATGT	360	0.0	18.5	28
GCAGCGT	90	2.152703E-6	18.5	1
>>END_MODULE