FastQCFastQC Report
Fri 10 Feb 2017
ERR1630474.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630474.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328773
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66952.036359433408461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53081.6144878076971043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37341.135738032016011No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT29900.9094420770562059No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26290.7996398731039349No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT24540.7464116578916152TruSeq Adapter, Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG13160.40027617839664453No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC12710.3865889230563337No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC10920.3321440629248752No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.2110878934705709No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.2031797014961691No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5960.18128009295167183No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC5930.18036760926231776RNA PCR Primer, Index 8 (95% over 23bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.1800634480325331No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5870.17854264188360966No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5610.17063444990920787No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5320.16181377424545204No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5100.15512222719018898No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.1487348413647106No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.13261429618612236No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG4320.1313976512669836No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4110.12501026544150523No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3870.11771039592667282No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3860.11740623469688811No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3500.10645643042463948No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG3310.1006773670587305No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.10006904459916113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGCC250.005490707229.63
CCGTCTT3300.028.03030437
TAGACAG405.927433E-527.755
GCCGTCT3450.026.81159436
TACCACG551.8973065E-523.5454544
TCTGACT400.001928271523.12523
GTATTAG400.001928271523.1251
TATACAC4250.022.6352943
AGTACTG502.6964195E-422.25
ACCACGT603.715878E-521.5833345
CACGTGT705.083659E-621.1428597
TTATACA4600.020.9130442
ACTGCCA450.00382008720.55555527
ACATGTT450.00382008720.55555529
CTACACT450.00382008720.5555554
ACTTTAT450.00382008720.5555558
CACTTAT450.00382008720.5555551
CGCCAGA450.00382008720.5555551
ATGCCGT4700.020.07446734
ACGTGTC759.2328155E-619.7333348
Produced by FastQC (version 0.11.5)