##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630474.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	328773
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.858930021625863	33.0	31.0	34.0	30.0	34.0
2	31.97550589616544	33.0	31.0	34.0	30.0	34.0
3	32.00125922749131	33.0	31.0	34.0	30.0	34.0
4	35.65741408205662	37.0	35.0	37.0	33.0	37.0
5	35.682860210540404	37.0	35.0	37.0	33.0	37.0
6	35.761969504795104	37.0	35.0	37.0	35.0	37.0
7	35.71532638020762	37.0	35.0	37.0	33.0	37.0
8	35.75737058700076	37.0	35.0	37.0	33.0	37.0
9	37.400680712832255	39.0	37.0	39.0	34.0	39.0
10	37.31581364649774	39.0	37.0	39.0	34.0	39.0
11	37.45921349989202	39.0	37.0	39.0	35.0	39.0
12	37.39338084331742	39.0	37.0	39.0	34.0	39.0
13	37.47531883700912	39.0	37.0	39.0	35.0	39.0
14	38.58065291249586	40.0	38.0	41.0	34.0	41.0
15	38.54544016692368	40.0	38.0	41.0	34.0	41.0
16	38.422586404601354	40.0	38.0	41.0	34.0	41.0
17	38.49534481237814	40.0	38.0	41.0	34.0	41.0
18	38.58348769515745	40.0	38.0	41.0	34.0	41.0
19	38.648474783513244	40.0	38.0	41.0	34.0	41.0
20	38.65779124198155	40.0	38.0	41.0	34.0	41.0
21	38.59622596746083	40.0	38.0	41.0	34.0	41.0
22	38.49482773828751	40.0	38.0	41.0	34.0	41.0
23	38.46209694835038	40.0	38.0	41.0	34.0	41.0
24	38.386160055722335	40.0	38.0	41.0	34.0	41.0
25	38.30642723094658	40.0	38.0	41.0	34.0	41.0
26	38.009581078738215	40.0	37.0	41.0	33.0	41.0
27	37.82044450122121	40.0	37.0	41.0	33.0	41.0
28	37.69273936728381	40.0	36.0	41.0	33.0	41.0
29	37.51804740656927	40.0	36.0	41.0	33.0	41.0
30	37.30914034911626	39.0	36.0	41.0	32.0	41.0
31	37.10577510926992	39.0	35.0	41.0	32.0	41.0
32	36.868605390345316	39.0	35.0	41.0	31.0	41.0
33	36.7074455627439	39.0	35.0	41.0	31.0	41.0
34	36.527263491831754	39.0	35.0	41.0	31.0	41.0
35	36.34334023779325	39.0	35.0	41.0	30.0	41.0
36	36.16056671320334	39.0	35.0	41.0	30.0	41.0
37	35.79080703099099	38.0	35.0	41.0	29.0	41.0
38	35.55705608428916	38.0	35.0	40.0	27.0	41.0
39	35.284859158142545	38.0	35.0	40.0	26.0	41.0
40	34.979329202823834	38.0	35.0	40.0	24.0	41.0
41	34.664631828039404	38.0	34.0	40.0	22.0	41.0
42	34.36478968771767	38.0	34.0	40.0	21.0	41.0
43	33.43483497732478	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	1.0
12	1.0
13	2.0
14	0.0
15	0.0
16	5.0
17	7.0
18	15.0
19	41.0
20	100.0
21	141.0
22	312.0
23	553.0
24	815.0
25	1391.0
26	2083.0
27	2997.0
28	4149.0
29	5455.0
30	7050.0
31	8695.0
32	10588.0
33	13362.0
34	17140.0
35	21788.0
36	29712.0
37	49817.0
38	68029.0
39	84520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.549056643945825	20.143077442490714	13.486508928652901	20.82135698491056
2	18.83487999318679	22.137766787418673	34.06636189711442	24.960991322280115
3	21.971390594726454	25.488406894726758	31.282982483354775	21.257220027192012
4	13.27542103518233	17.70644183068561	31.80401066997594	37.214126464156124
5	11.880233474159983	40.64719426473585	33.31112956355905	14.161442697545116
6	31.325869216754416	32.73991477402342	17.29399920309758	18.64021680612459
7	26.304471474239065	34.02286684125521	20.679617851830898	18.993043832674825
8	31.446317063749152	30.63755235375168	19.438031711849817	18.478098870649355
9	26.87781539238319	11.506419322754606	21.95496588831808	39.66079939654412
10	20.289683155246934	28.55921867063293	28.07286486420722	23.078233309912918
11	31.085277683994732	23.328558002025716	20.066428812584974	25.51973550139458
12	20.976175050870964	31.001633345803942	26.45746457282076	21.56472703050433
13	31.362672725558365	18.958673613709152	27.927779957599924	21.750873703132555
14	23.035042415283495	23.064850215802394	27.86421026057492	26.03589710833919
15	29.472006521216766	24.38156417954029	25.454644998220655	20.691784301022288
16	24.310694613000457	24.459125293135386	28.50599045542061	22.724189638443544
17	19.838003729016677	30.513454571999528	27.72490441733354	21.923637281650258
18	20.73832096917934	23.42497711186746	31.020491342050594	24.816210576902606
19	21.72380335368172	26.55540448881143	32.199724429925816	19.521067727581038
20	21.446408312118088	22.795363366213163	33.95260559717495	21.8056227244938
21	24.078011272215175	23.51409635219437	32.23196552028299	20.17592685530746
22	22.86532044906364	25.918490873642302	29.970830938063646	21.24535773923041
23	21.900216866956836	25.501181666377715	31.48889963591901	21.10970183074644
24	21.298890115672517	27.01529626824587	31.32009015338851	20.365723462693104
25	20.7212879403114	26.845270140796234	30.97030473913612	21.463137179756245
26	21.71650348416689	26.788087829596712	31.621209770875346	19.874198915361056
27	20.413780936999085	27.877289193455667	30.181918831534222	21.52701103801103
28	19.2278563020686	26.85652410629827	33.427319153336796	20.48830043829633
29	20.904088839411997	27.351698588387734	31.852676466741492	19.891536105458783
30	19.211431595660226	27.716692064129354	33.25120980129147	19.82066653891895
31	18.88445827364169	28.311631429588196	32.17660817646218	20.627302120307935
32	18.896928884062863	28.288211014894777	31.83077685819699	20.984083242845365
33	18.208916182289908	28.94976168967646	33.19646077993023	19.644861348103404
34	19.22329388362183	28.739586279895246	31.72097465424472	20.316145182238202
35	19.418261231913814	28.721336606108167	32.0333482372336	19.82705392474443
36	19.215081530417642	29.560213277854324	29.774038622392958	21.450666569335073
37	18.55048924333811	29.006944000875983	30.970913061595688	21.471653694190216
38	18.684624345673154	26.863215653353528	32.27302728630392	22.179132714669393
39	19.09463368342291	27.849306360315474	31.328910829052266	21.72714912720935
40	17.748111919166114	27.38758961350233	33.24725570530427	21.617042762027296
41	17.900800856518025	27.918959281936168	31.096835810726546	23.083404050819258
42	17.767274076642547	27.751062283095024	32.839375496163	21.642288144099425
43	17.464633653006786	27.626356178883306	32.413245613234665	22.495764554875247
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	44.0
2	76.0
3	306.5
4	537.0
5	537.0
6	879.5
7	1222.0
8	1319.0
9	1416.0
10	1904.0
11	2392.0
12	2392.0
13	4599.5
14	6807.0
15	9674.0
16	12541.0
17	11086.5
18	9632.0
19	9632.0
20	11223.5
21	12815.0
22	9251.5
23	5688.0
24	5285.5
25	4883.0
26	4883.0
27	5230.0
28	5577.0
29	5985.5
30	6394.0
31	7121.5
32	7849.0
33	7849.0
34	9052.5
35	10256.0
36	11045.0
37	11834.0
38	13607.5
39	15381.0
40	15381.0
41	16849.0
42	18317.0
43	19922.0
44	21527.0
45	22024.5
46	22522.0
47	22522.0
48	24302.0
49	26082.0
50	26599.0
51	27116.0
52	22212.0
53	17308.0
54	17308.0
55	18001.5
56	18695.0
57	16369.5
58	14044.0
59	12114.0
60	10184.0
61	10184.0
62	9525.0
63	8866.0
64	8160.0
65	7454.0
66	6951.5
67	6449.0
68	6449.0
69	5726.0
70	5003.0
71	4389.0
72	3775.0
73	3211.5
74	2648.0
75	2648.0
76	2114.0
77	1580.0
78	1299.0
79	1018.0
80	747.0
81	476.0
82	476.0
83	355.0
84	234.0
85	162.0
86	90.0
87	66.0
88	42.0
89	42.0
90	30.5
91	19.0
92	13.0
93	7.0
94	6.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	328773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.29224783552092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.46775776630236	33.97045616316383
2	11.019370841850325	9.54106666953794
3	3.6192830222739953	4.70060692761537
4	1.7820105181925519	3.0858896399638804
5	1.0320061353826422	2.233893269038175
6	0.7012388417959388	1.8214923438554058
7	0.488037571969955	1.4789770463138419
8	0.35457867195916604	1.228040619491687
9	0.31716486550308276	1.2357701965871208
>10	1.872521384042088	15.281310590503422
>50	0.2184713198078296	6.540438742248335
>100	0.11487362944734265	8.868050216083878
>500	0.006342715736356343	1.6271007982651662
>1k	0.004933223350499377	4.724785854478599
>5k	0.0014094923858569651	3.662120922853327
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6695	2.036359433408461	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5308	1.6144878076971043	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3734	1.135738032016011	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	2990	0.9094420770562059	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2629	0.7996398731039349	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	2454	0.7464116578916152	TruSeq Adapter, Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	1316	0.40027617839664453	No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	1271	0.3865889230563337	No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	1092	0.3321440629248752	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	694	0.2110878934705709	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	668	0.2031797014961691	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	596	0.18128009295167183	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	593	0.18036760926231776	RNA PCR Primer, Index 8 (95% over 23bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	592	0.1800634480325331	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	587	0.17854264188360966	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	561	0.17063444990920787	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	532	0.16181377424545204	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	510	0.15512222719018898	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	489	0.1487348413647106	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	436	0.13261429618612236	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	432	0.1313976512669836	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	411	0.12501026544150523	No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	387	0.11771039592667282	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	386	0.11740623469688811	No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA	350	0.10645643042463948	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	331	0.1006773670587305	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.10006904459916113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0012166449191387371	0.0
4	0.0	0.0	0.0	0.0018249673787081055	0.0
5	0.0	0.0	0.0	0.0018249673787081055	0.0
6	0.0	0.0	0.0	0.0018249673787081055	0.0
7	0.0	0.0	0.0	0.0027374510680621585	0.0
8	0.0	0.0	0.0	0.0030416122978468426	0.0
9	0.0	0.0	0.0	0.004258257216985579	0.0
10	0.0	0.0	0.0	0.005779063365909001	0.0
11	0.0	0.0	0.0	0.006083224595693685	0.0
12	0.0	0.0	0.0	0.007299869514832422	0.0
13	0.0	0.0	0.0	0.007299869514832422	0.0
14	0.0	0.0	0.0	0.009124836893540529	0.0
15	0.0	0.0	0.0	0.011558126731818002	3.041612297846843E-4
16	0.0	0.0	0.0	0.01916215747643511	3.041612297846843E-4
17	0.0	0.0	0.0	0.034674380195454005	3.041612297846843E-4
18	0.0	0.0	0.0	0.03923679864222427	3.041612297846843E-4
19	0.0	0.0	0.0	0.050186602914472904	3.041612297846843E-4
20	0.0	0.0	0.0	0.05626982751016659	3.041612297846843E-4
21	0.0	0.0	0.0	0.06995708285047739	3.041612297846843E-4
22	0.0	0.0	0.0	0.10736891411399355	3.041612297846843E-4
23	0.0	0.0	0.0	0.1545139047306196	3.041612297846843E-4
24	0.0	0.0	0.0	0.2335958244746375	3.041612297846843E-4
25	0.0	0.0	0.0	0.2512371758021492	3.041612297846843E-4
26	0.0	0.0	0.0	0.29351558674222034	3.041612297846843E-4
27	0.0	0.0	0.0	0.3245400321802581	3.041612297846843E-4
28	0.0	0.0	0.0	0.3695558941883914	3.041612297846843E-4
29	0.0	0.0	0.0	0.43282142998360573	3.041612297846843E-4
30	0.0	0.0	0.0	0.5517484708294172	3.041612297846843E-4
31	0.0	0.0	0.0	0.9538496166047699	3.041612297846843E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGGCC	25	0.0054907072	29.6	3
CCGTCTT	330	0.0	28.030304	37
TAGACAG	40	5.927433E-5	27.75	5
GCCGTCT	345	0.0	26.811594	36
TACCACG	55	1.8973065E-5	23.545454	4
TCTGACT	40	0.0019282715	23.125	23
GTATTAG	40	0.0019282715	23.125	1
TATACAC	425	0.0	22.635294	3
AGTACTG	50	2.6964195E-4	22.2	5
ACCACGT	60	3.715878E-5	21.583334	5
CACGTGT	70	5.083659E-6	21.142859	7
TTATACA	460	0.0	20.913044	2
ACTGCCA	45	0.003820087	20.555555	27
ACATGTT	45	0.003820087	20.555555	29
CTACACT	45	0.003820087	20.555555	4
ACTTTAT	45	0.003820087	20.555555	8
CACTTAT	45	0.003820087	20.555555	1
CGCCAGA	45	0.003820087	20.555555	1
ATGCCGT	470	0.0	20.074467	34
ACGTGTC	75	9.2328155E-6	19.733334	8
>>END_MODULE