FastQCFastQC Report
Fri 10 Feb 2017
ERR1630473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748483
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118120.6755570400169747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT111210.6360370675608513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86820.4965447190507429No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74650.42694152588272233No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.13285802607174332No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.10586319683977483No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18280.1045477708390645No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.10134499448950891No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGG400.001931587223.12500228
ACACCGT502.702883E-422.26
GCCGTCT2000.018.536
TTAACGG1258.587449E-917.7635
TAACGGC1301.3944373E-817.07692336
AGTCGGT3150.017.03174611
CAGTCGG3050.016.98360610
ATACCGT1751.3278623E-1015.8571436
GGGCGTT700.002593218815.8571427
TAGACAG2003.6379788E-1215.7255
CGGTGAT2950.015.67796614
CGTCGTA1701.4879333E-915.23529410
TACCGTC1701.4879333E-915.2352947
GGTATCA68900.015.0362851
GCAGTCG3450.015.0144939
GTGCGTA750.004105543714.815
CGCCTTA2700.014.38888925
GTCGGTG3600.014.38888912
CGTATGC2450.014.34693831
CTAGGTA5700.014.2807024
Produced by FastQC (version 0.11.5)