FastQCFastQC Report
Fri 10 Feb 2017
ERR1630472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683993
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT218943.20090995083283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT192452.813625285638888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134501.9663943929250738No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104471.5273548121106504No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT55790.8156516221657238No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT34730.5077537343218425Illumina PCR Primer Index 10 (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.3995654926293105No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA23980.3505883832144481No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG23050.3369917528395759No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22110.32324892213809203No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.3057048829447085No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA20150.29459365812223226No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20010.2925468535496708No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC19610.2866988404852096No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA16610.24283874250175075No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC14890.21769228632456764No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14120.20643486117547988No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13780.20146405007068785No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA13490.1972242405989535No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13430.19634703863928432No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA12350.18055740336523912No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA12020.17573279258705862No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10730.15687295045417132No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.14927053347037175No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA9830.14371492105913364No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA9080.1327498965632689No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG8880.12982589003103834No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA8460.12368547631335408No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.12076146978112348No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC7910.11564445834971995Illumina PCR Primer Index 10 (95% over 22bp)
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC7870.11505965704327384No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA7790.1138900544303816No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7420.10848064234575501No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA7280.10643383777319357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT7210.10541043548691288No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6930.10131682634179004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCTC303.5979683E-430.8333325
ACACTGC303.5979683E-430.8333326
GTACTTA250.005494806429.61
ATACACT803.274181E-1127.7500024
GCCGTCT3650.027.36986436
CCGTCTT3800.027.26315937
GATACAC951.0913936E-1125.315793
CGTCTTC901.4188117E-1024.66666637
CAAATTT601.3360841E-624.66666621
GACTCGC451.3224468E-424.66666611
TGACTCG451.3224468E-424.66666610
CAGCGTC701.9205618E-723.7857132
AGCGTCA701.9205618E-723.7857133
AATACTG1650.023.5454545
ACACGTA400.001930391623.12500223
GTACTTG400.001930391623.1250026
ACTAAAT400.001930391623.1250023
GAGGCAC1250.022.1999997
TTGGTAA1550.021.4838733
CAGTCGG4950.021.30302810
Produced by FastQC (version 0.11.5)