FastQCFastQC Report
Fri 10 Feb 2017
ERR1630471.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630471.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820217
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42220.23195036635741784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36780.2020638198632361No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27450.150806195085531No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.10822885403223902No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA19290.1059763753442584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.019.2615011
AAGACGG4200.017.1785725
GTATTGA1201.0421536E-716.9583321
GGATATA4800.016.9583322
TTTCGGA3600.016.44444530
CGTACAC904.448251E-516.4444453
ATACCGT2950.016.3050846
ACACCGT803.3839224E-416.18756
TACCGTC2750.016.1454547
CTTATAC9450.016.0529137
GGGATAT5200.016.0096151
TTCGGAA3700.016.031
TATACTG1751.3278623E-1015.8571425
CAAGACG4550.015.450554
CGAACGA1954.1836756E-1115.17948716
CGCAAGA4600.014.8804342
GTGTACG750.00410562914.81
TACACGG750.00410562914.85
TACGTTA1001.0939207E-414.819
GCGTTAT2900.014.6724141
Produced by FastQC (version 0.11.5)