##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630471.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1820217
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.922956988095375	33.0	31.0	34.0	30.0	34.0
2	32.09176927805861	33.0	31.0	34.0	30.0	34.0
3	32.19962894534003	34.0	31.0	34.0	30.0	34.0
4	35.80388766833845	37.0	35.0	37.0	35.0	37.0
5	35.77515318228541	37.0	35.0	37.0	35.0	37.0
6	35.80469251743062	37.0	35.0	37.0	35.0	37.0
7	35.77867254288912	37.0	35.0	37.0	35.0	37.0
8	35.76586582808533	37.0	35.0	37.0	33.0	37.0
9	37.488218162999246	39.0	37.0	39.0	35.0	39.0
10	37.37625953389074	39.0	37.0	39.0	34.0	39.0
11	37.49270554005374	39.0	37.0	39.0	35.0	39.0
12	37.41459507300503	39.0	37.0	39.0	34.0	39.0
13	37.46269043745883	39.0	37.0	39.0	35.0	39.0
14	38.72284458391499	40.0	38.0	41.0	35.0	41.0
15	38.69930947793587	40.0	38.0	41.0	35.0	41.0
16	38.67987443255392	40.0	38.0	41.0	34.0	41.0
17	38.654592831514044	40.0	38.0	41.0	34.0	41.0
18	38.633360747647124	40.0	38.0	41.0	34.0	41.0
19	38.652912262658795	40.0	38.0	41.0	34.0	41.0
20	38.63305913525695	40.0	38.0	41.0	34.0	41.0
21	38.556406736119925	40.0	38.0	41.0	34.0	41.0
22	38.506278097611435	40.0	38.0	41.0	34.0	41.0
23	38.477486475513636	40.0	38.0	41.0	34.0	41.0
24	38.42283914500304	40.0	38.0	41.0	34.0	41.0
25	38.38210004631316	40.0	38.0	41.0	34.0	41.0
26	38.18595694908904	40.0	38.0	41.0	33.0	41.0
27	38.075310251470015	40.0	38.0	41.0	33.0	41.0
28	37.97747466373515	40.0	37.0	41.0	33.0	41.0
29	37.877667882455775	40.0	37.0	41.0	33.0	41.0
30	37.80695763197465	40.0	37.0	41.0	33.0	41.0
31	37.771931038991504	40.0	37.0	41.0	33.0	41.0
32	37.67936625138651	40.0	37.0	41.0	32.0	41.0
33	37.648279847952196	40.0	37.0	41.0	32.0	41.0
34	37.57513197602264	40.0	37.0	41.0	32.0	41.0
35	37.51687848207109	40.0	37.0	41.0	32.0	41.0
36	37.48379781092034	40.0	37.0	41.0	32.0	41.0
37	37.30417856772022	40.0	36.0	41.0	31.0	41.0
38	37.1992234991762	39.0	36.0	41.0	31.0	41.0
39	37.148121899751516	39.0	36.0	41.0	31.0	41.0
40	37.04902547333642	39.0	36.0	41.0	31.0	41.0
41	36.92763994622619	39.0	35.0	41.0	31.0	41.0
42	36.84802910861727	39.0	35.0	41.0	31.0	41.0
43	35.9666946303655	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	6.0
11	2.0
12	1.0
13	2.0
14	4.0
15	4.0
16	11.0
17	40.0
18	70.0
19	148.0
20	287.0
21	596.0
22	1021.0
23	1820.0
24	2936.0
25	4670.0
26	7169.0
27	10402.0
28	14773.0
29	20497.0
30	27822.0
31	36165.0
32	46775.0
33	60254.0
34	80154.0
35	107221.0
36	150344.0
37	227662.0
38	421022.0
39	598334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04974791467171	18.368139622913095	11.709977436756166	25.872135025659027
2	19.697926126390424	20.28664714152214	33.73790048109648	26.277526250990952
3	20.76939178130959	22.350741697281148	28.610544786693016	28.269321734716247
4	14.81653011701352	14.963435678273523	33.192965454118934	37.02706875059402
5	14.973928932649239	36.2678186172308	33.86788498294434	14.890367467175617
6	36.56064084666828	33.56336085203028	15.02815323667453	14.847845064626911
7	30.303090235944396	29.524281994948954	20.655119691773013	19.517508077333638
8	27.77278753027798	32.64094336004993	19.488885116444905	20.09738399322718
9	27.012823196355164	13.111348811707616	18.974715652034895	40.901112339902326
10	17.178995691173085	26.032445582037745	32.5599640042918	24.228594722497373
11	36.80923757991492	20.756206540209217	20.873555186002548	21.561000693873314
12	22.288716125604804	24.72419497235769	28.088024669586098	24.89906423245141
13	30.424284577058668	19.074099406828964	24.95960646450396	25.54200955160841
14	23.758046430727763	20.51436724302652	24.246339859478294	31.481246466767427
15	26.611167789335006	27.073640120930637	21.40233829263214	24.912853797102212
16	26.51672849995358	25.6274938647425	23.143064810404475	24.712712824899448
17	24.679969476166853	25.72698749654574	24.55564364029124	25.037399386996167
18	24.55383066963994	24.1943130956364	26.067166716935397	25.18468951778826
19	25.022291298235324	24.646292172856313	25.6667748955207	24.664641633387667
20	25.548327479635667	23.89473343013498	25.556842947846327	25.000096142383022
21	25.90949320877676	24.482135921156654	24.879176493791675	24.729194376274915
22	25.839556492440185	24.178930314352627	24.929060655954757	25.052452537252428
23	24.903404374313613	24.33836185465799	25.460920318841108	25.297313452187293
24	25.01014988872206	24.940817495935924	25.148649858780576	24.90038275656144
25	25.55799665644261	24.287433860907793	25.09360147718651	25.060968005463085
26	25.070142735728762	25.439329486539243	25.050639566601124	24.43988821113087
27	25.72149364608725	24.21656318999328	24.620526014206	25.44141714971347
28	24.673706486644175	24.605527802454322	25.579642427249063	25.141123283652444
29	24.27320478822031	24.896591999745084	25.769235206571523	25.060968005463085
30	24.16525062671099	25.584531954157114	25.809669945946005	24.440547473185887
31	25.100743482782544	25.238364436767704	24.748367914375045	24.912524166074704
32	24.32264944234671	24.813854611840235	25.141892422716634	25.72160352309642
33	24.15107649252809	24.6394248597832	25.684794725024545	25.524703922664166
34	25.283304133518147	24.379016348050808	25.3714804333769	24.966199085054143
35	25.015039415630113	24.405881276792822	26.23390507835055	24.345174229226515
36	24.194532849654742	25.108434873424436	25.51992427276528	25.177108004155546
37	25.09975458970002	23.98928259652558	25.785277249910315	25.125685563864085
38	24.238154022295145	23.915939692904747	26.51002600239422	25.33588028240589
39	24.614537717206243	23.92154342037241	26.163583792481887	25.30033506993946
40	23.86297897448491	24.07361320106339	26.94453463515614	25.11887318929556
41	23.264698659555428	24.013949985084196	27.594457144395424	25.126894210964956
42	24.146186965620036	23.956759001811324	27.23444512385062	24.662608908718024
43	23.37924544161493	23.341392811955938	27.51726854545365	25.76209320097549
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	70.0
1	86.5
2	103.0
3	332.0
4	561.0
5	561.0
6	809.0
7	1057.0
8	1104.0
9	1151.0
10	1579.0
11	2007.0
12	2007.0
13	3578.0
14	5149.0
15	7194.5
16	9240.0
17	8945.0
18	8650.0
19	8650.0
20	11089.5
21	13529.0
22	13544.0
23	13559.0
24	15914.5
25	18270.0
26	18270.0
27	21305.0
28	24340.0
29	28993.5
30	33647.0
31	39526.0
32	45405.0
33	45405.0
34	50886.5
35	56368.0
36	64161.5
37	71955.0
38	80295.5
39	88636.0
40	88636.0
41	93977.5
42	99319.0
43	100968.0
44	102617.0
45	108148.0
46	113679.0
47	113679.0
48	117574.5
49	121470.0
50	127171.0
51	132872.0
52	140354.0
53	147836.0
54	147836.0
55	141926.0
56	136016.0
57	131092.0
58	126168.0
59	116117.0
60	106066.0
61	106066.0
62	99868.5
63	93671.0
64	84346.5
65	75022.0
66	66312.5
67	57603.0
68	57603.0
69	49228.0
70	40853.0
71	35775.0
72	30697.0
73	24202.0
74	17707.0
75	17707.0
76	13624.0
77	9541.0
78	7934.0
79	6327.0
80	4974.5
81	3622.0
82	3622.0
83	2800.5
84	1979.0
85	1723.5
86	1468.0
87	1326.0
88	1184.0
89	1184.0
90	947.5
91	711.0
92	389.0
93	67.0
94	42.5
95	18.0
96	18.0
97	12.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1820217.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.835032115011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.92537509024508	47.11720702567956
2	8.358937480710644	9.167252104042747
3	2.1730363486129565	3.574755538898332
4	0.9512666413519832	2.0865094731389866
5	0.5517330901188744	1.5127150857791372
6	0.34240724329247485	1.1265547309413146
7	0.24065462021137063	0.9237412679541399
8	0.17789639138527993	0.7803963467805115
9	0.13349271888590944	0.6588069774506082
>10	0.8944101305708542	9.930043931346818
>50	0.12492297599553037	4.799420634961533
>100	0.11423372132120058	12.856671452181098
>500	0.009627763281013384	3.6226494268300664
>1k	0.002005784016877788	1.843276004015092
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4222	0.23195036635741784	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3678	0.2020638198632361	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2745	0.150806195085531	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1970	0.10822885403223902	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1929	0.1059763753442584	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.845695320942503E-4	0.0
3	0.0	0.0	0.0	4.944465412640361E-4	0.0
4	0.0	0.0	0.0	7.142005596036077E-4	0.0
5	0.0	0.0	0.0	8.790160733582864E-4	0.0
6	0.0	0.0	0.0	8.790160733582864E-4	0.0
7	0.0	0.0	0.0	0.0012086471008676439	0.0
8	0.0	0.0	0.0	0.0013185241100374296	0.0
9	0.0	0.0	0.0	0.0030216177521691094	0.0
10	0.0	0.0	0.0	0.004504957375961217	0.0
11	0.0	5.49385045848929E-5	0.0	0.004889526908055468	0.0
12	0.0	5.49385045848929E-5	0.0	0.005658665972243969	0.0
13	0.0	5.49385045848929E-5	0.0	0.005823481485998648	0.0
14	0.0	5.49385045848929E-5	0.0	0.006317928027262684	0.0
15	0.0	5.49385045848929E-5	0.0	0.006922251577696505	0.0
16	0.0	5.49385045848929E-5	0.0	0.008515468210658399	0.0
17	0.0	5.49385045848929E-5	0.0	0.010053746339035401	0.0
18	0.0	5.49385045848929E-5	0.0	0.011042639421563473	0.0
19	0.0	5.49385045848929E-5	0.0	0.013020425586619618	0.0
20	0.0	5.49385045848929E-5	0.0	0.014833396237921083	0.0
21	0.0	5.49385045848929E-5	0.0	0.01823958352218444	0.0
22	0.0	5.49385045848929E-5	0.0	0.02290935641190034	0.0
23	0.0	5.49385045848929E-5	0.0	0.02955691546667238	0.0
24	0.0	1.098770091697858E-4	0.0	0.03977547731946246	0.0
25	0.0	1.098770091697858E-4	0.0	0.04219277152119775	0.0
26	0.0	1.098770091697858E-4	0.0	0.04807119151178129	0.0
27	0.0	1.098770091697858E-4	0.0	0.056147151685760545	0.0
28	0.0	1.648155137546787E-4	0.0	0.08147380229939617	0.0
29	0.0	1.648155137546787E-4	0.0	0.12262274223348095	0.0
30	0.0	1.648155137546787E-4	0.0	0.18047298756137317	0.0
31	0.0	1.648155137546787E-4	0.0	0.37896580462659124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2065	0.0	19.261501	1
AAGACGG	420	0.0	17.178572	5
GTATTGA	120	1.0421536E-7	16.958332	1
GGATATA	480	0.0	16.958332	2
TTTCGGA	360	0.0	16.444445	30
CGTACAC	90	4.448251E-5	16.444445	3
ATACCGT	295	0.0	16.305084	6
ACACCGT	80	3.3839224E-4	16.1875	6
TACCGTC	275	0.0	16.145454	7
CTTATAC	945	0.0	16.05291	37
GGGATAT	520	0.0	16.009615	1
TTCGGAA	370	0.0	16.0	31
TATACTG	175	1.3278623E-10	15.857142	5
CAAGACG	455	0.0	15.45055	4
CGAACGA	195	4.1836756E-11	15.179487	16
CGCAAGA	460	0.0	14.880434	2
GTGTACG	75	0.004105629	14.8	1
TACACGG	75	0.004105629	14.8	5
TACGTTA	100	1.0939207E-4	14.8	19
GCGTTAT	290	0.0	14.672414	1
>>END_MODULE