FastQCFastQC Report
Fri 10 Feb 2017
ERR1630470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798456
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101140.5623712784744247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT98350.546857971504446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71480.3974520366358699No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64520.3587521740871058No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC34740.19316569323908953No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT27210.1512964453953836No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA26870.14940593486857615No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA23480.1305564328512902No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG22550.12538533052796397No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG21440.11921336969044558No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.1107060723198121No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG19090.10614660575515887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1601.8189894E-1218.528
TTAACGG1351.1532393E-917.81481635
TGCCGTC1800.017.47222135
CGCAATA1705.456968E-1217.41176436
GCCGTCT1705.456968E-1217.41176436
TAACGGC1352.2215318E-816.44444536
TCTCGTA1951.8189894E-1216.12820628
ATGCCGT1951.8189894E-1216.12820634
CTCGTAT1851.8189894E-1116.029
TAGCGTA1659.749783E-1015.696977
GTGTTAG2400.015.4166671
GTAAACG2055.456968E-1215.34146327
GCAGTCG2300.015.2826099
ATAGCGT1701.4879333E-915.2352946
ACGCTTC2201.8189894E-1215.13636431
GCTTTAT9250.015.01
ACCTAGG12200.014.8606551
CGTATGC2006.184564E-1114.831
AAGACGG3000.014.85
ACGAGCG750.004105604314.85
Produced by FastQC (version 0.11.5)