FastQCFastQC Report
Fri 10 Feb 2017
ERR1630469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862529
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16520.19152979204177484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14250.16521183635564718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9920.11501062572968561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG251.2333701E-437.05
ATATCGC358.867593E-426.428576
TACACCG551.9019453E-523.5454545
GTGTCGA400.001930798223.12500211
CGACGAC450.003825045720.55555533
GTGCTAG1001.288754E-820.351
GGTATCA10650.018.9342731
CGAATTG500.00703349318.514
TCCGTAG1800.018.515
CTCCGTA1850.018.014
GTAACAC752.0665131E-417.2666663
TCTATAC1107.802046E-716.8181823
TTTCGGA1352.2168024E-816.44444330
CCGTAGA2050.016.24390416
TACACAG1606.2755134E-1016.18755
TCCGAAT700.002591790415.85714212
CGAATTA700.002591790415.85714215
TGTAAGC700.002591790415.8571422
GTGCGTA957.056407E-515.57894815
TGCGTAC957.056407E-515.57894816
Produced by FastQC (version 0.11.5)