FastQCFastQC Report
Fri 10 Feb 2017
ERR1630468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733129
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132411.806094152597974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT117441.6019008932943588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92551.2623972043119287No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66830.9115721789753235No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT20470.2792141628553774No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA19190.26175475257424No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.24852379322056556No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.24579576036413783No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17730.24184011272231762No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC16580.2261539237978582No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC16160.22042505479936003No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC15250.20801250530261386No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT15200.20733049708850693No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT14900.20323844780386532TruSeq Adapter, Index 5 (95% over 21bp)
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC14670.20010121001897344No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC14230.19409953773483246No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT13180.17977736523858695No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA13150.17936816031012276No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG12830.1750033077398384No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG12690.173093684740339No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA12680.17295728309751762No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12130.16545519274234138No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11750.1602719303151287No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC11480.1565890859589513No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG11380.15522506953073742No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA11260.1535882498168808No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC10050.13708365103549308No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC9890.13490122475035088No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9790.13353720832213703No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC9710.13244599517956596No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC9210.12562591303849663No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9210.12562591303849663No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG9040.12330708511053307No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8970.12235227361078337No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG8950.1220794703251406No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8860.12085185553974813No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8740.11921503582589148No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT8710.11880583089742733No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT8570.11689620789792793No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC8420.11485018325560713No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC8350.11389537175585743No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.11280415861328634No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA8240.11239495368482219No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC8170.11144014218507248No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC8110.11062173232814417No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT7880.10748449454325228No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7650.1043472567583604No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG7570.10325604361578931No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.10202842883039684No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG7360.10039160911654019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2400.028.52083236
ACACGCC358.8663195E-426.4285733
GAAGTCA358.8663195E-426.428576
CCGTCTT2650.025.83018937
CCGCTTA701.9209438E-723.78571325
TTTAGCG400.001930523823.12526
TAGAACT400.001930523823.1254
ATTCGCC400.001930523823.12525
CGGGGTG400.001930523823.12531
TCATGGA400.001930523823.1252
ATGCCGT3000.022.81666834
TCCTAAG603.7239173E-521.5833322
GTCAGTC603.7239173E-521.5833327
TGCCGTC3200.021.39062535
GAGATCT806.9530506E-720.812534
TATGCCG3300.020.74242433
GGTAAGT450.003824506420.55555528
TCGGGTA450.003824506420.55555525
TAAGTTC450.003824506420.55555530
TCATCCA450.003824506420.55555523
Produced by FastQC (version 0.11.5)