FastQCFastQC Report
Fri 10 Feb 2017
ERR1630467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014275
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36230.3572009563481304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32190.3173695496783417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24980.24628429173547609No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC21570.21266421828399595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.18988932981686427No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT19220.18949495945379705No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA15340.15124103423627713No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA14090.13891696039042667No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG12850.12669147913534298No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG11940.11771955337556382No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG11500.11338147938182445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG358.8686746E-426.428575
GTAGTGT502.701799E-422.1999996
GGTATCA16050.020.5171341
CCTATGC801.6163653E-518.58
CAAGACG2150.018.0697674
GTTTTCG1750.017.9714328
ATACCGT1258.574716E-917.766
TTTCGGA1900.017.52631630
CTAGCGG953.6055408E-617.52631629
TTTTCGG1800.017.47222129
ACACCGG752.0669641E-417.2666666
AGACGGA2150.017.2093036
AAGACGG2050.017.1463435
GACGGAC1950.017.0769237
CAATACG650.001579760517.0769234
GCCGTCT1301.3924364E-817.07692336
CGCAAGA2100.016.7380942
TCTAGCG1005.87642E-616.6528
GATAACC904.4451277E-516.4444456
ATAGGAC2550.015.9607863
Produced by FastQC (version 0.11.5)