FastQCFastQC Report
Fri 10 Feb 2017
ERR1630466.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630466.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764514
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT127281.6648485181435528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT123321.613050905542606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91571.197754390370876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74470.9740828814122436No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19590.256241219912258No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19470.2546715952879869No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.23557449569268843No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.19921152523040783No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT14530.19005538158882634No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12750.16677261632880497No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA12420.16245614861205943No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10540.1378653628318121No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10250.13407210332315694No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT9960.13027884381450178TruSeq Adapter, Index 7 (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.1294940315023662No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA9660.12635478225382402No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT9060.11850665913246848No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8770.1147133996238133No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA8310.10869650523077407No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA8200.10725768265852556No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG7890.10320281904582519No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7830.10241800673368964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCC303.598361E-430.8333348
AAGTCTC303.598361E-430.8333347
TCCTAGA250.00549520729.5999981
GTACGAG250.00549520729.5999983
GCAGCGT951.0913936E-1125.315791
AGCGTCA901.4188117E-1024.6666683
AGTCGGT2750.023.54545611
TGCGATA400.001930598523.12500210
CGATAAG400.001930598523.12500212
CAGTCGG2800.022.46428710
GCCGTCT1750.022.236
TGCCGAT603.7241854E-521.58333412
GATACAC957.1358954E-921.4210533
GCAGTCG3050.020.622959
CCTAAAT450.00382465320.55555724
CCTCGCC2700.020.55555522
AGCGTCG555.1409274E-420.18181817
GCCTAAG555.1409274E-420.1818181
TCGGTGA3150.019.96825413
CTATGCC656.8982685E-519.9230779
Produced by FastQC (version 0.11.5)