##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630466.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	764514
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.941838867568155	33.0	31.0	34.0	30.0	34.0
2	32.036293645374705	33.0	31.0	34.0	30.0	34.0
3	32.08687087483028	34.0	31.0	34.0	30.0	34.0
4	35.71475080901069	37.0	35.0	37.0	33.0	37.0
5	35.72157867612627	37.0	35.0	37.0	35.0	37.0
6	35.79955631943954	37.0	35.0	37.0	35.0	37.0
7	35.75312420701256	37.0	35.0	37.0	35.0	37.0
8	35.798074332190126	37.0	35.0	37.0	35.0	37.0
9	37.456784310032255	39.0	37.0	39.0	35.0	39.0
10	37.38836960474236	39.0	37.0	39.0	34.0	39.0
11	37.523127110818116	39.0	37.0	39.0	35.0	39.0
12	37.48456274182029	39.0	37.0	39.0	35.0	39.0
13	37.52627813225134	39.0	37.0	39.0	35.0	39.0
14	38.70828395555869	40.0	38.0	41.0	35.0	41.0
15	38.66784126909383	40.0	38.0	41.0	34.0	41.0
16	38.58534441488318	40.0	38.0	41.0	34.0	41.0
17	38.6699929628496	40.0	38.0	41.0	34.0	41.0
18	38.69308475711367	40.0	38.0	41.0	35.0	41.0
19	38.769313576991394	40.0	38.0	41.0	35.0	41.0
20	38.75829742817005	40.0	38.0	41.0	35.0	41.0
21	38.675378083331374	40.0	38.0	41.0	34.0	41.0
22	38.586473236592134	40.0	38.0	41.0	34.0	41.0
23	38.51963731207015	40.0	38.0	41.0	34.0	41.0
24	38.426992834663594	40.0	38.0	41.0	34.0	41.0
25	38.34437302652404	40.0	38.0	41.0	34.0	41.0
26	38.04177817541601	40.0	37.0	41.0	33.0	41.0
27	37.83771781811713	40.0	36.0	41.0	33.0	41.0
28	37.66412779883691	40.0	36.0	41.0	33.0	41.0
29	37.488560052530104	39.0	36.0	41.0	33.0	41.0
30	37.283733195206366	39.0	35.0	41.0	32.0	41.0
31	37.078029964134075	39.0	35.0	41.0	32.0	41.0
32	36.82926015743335	39.0	35.0	41.0	31.0	41.0
33	36.66010301969617	39.0	35.0	41.0	31.0	41.0
34	36.45896478023947	39.0	35.0	41.0	31.0	41.0
35	36.22400636221181	39.0	35.0	41.0	30.0	41.0
36	36.005487146082345	39.0	35.0	41.0	30.0	41.0
37	35.6411432622555	38.0	35.0	41.0	27.0	41.0
38	35.32115697031055	38.0	35.0	41.0	25.0	41.0
39	35.047342494709056	38.0	35.0	40.0	24.0	41.0
40	34.7126815205477	38.0	34.0	40.0	21.0	41.0
41	34.333173754829865	38.0	34.0	40.0	18.0	41.0
42	33.99508969096707	38.0	33.0	40.0	15.0	41.0
43	33.08543074423751	37.0	33.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	5.0
12	6.0
13	3.0
14	3.0
15	6.0
16	11.0
17	16.0
18	48.0
19	109.0
20	256.0
21	434.0
22	815.0
23	1351.0
24	2197.0
25	3357.0
26	5015.0
27	7280.0
28	10225.0
29	13390.0
30	16833.0
31	20498.0
32	25372.0
33	31064.0
34	39369.0
35	49130.0
36	67192.0
37	109551.0
38	149751.0
39	211221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.58318879706585	19.832076325613397	13.429839087315601	20.154895790005153
2	17.357432303397978	23.991843184035872	34.58236212809707	24.06836238446909
3	22.26904935684631	26.80147126148115	29.909066413433894	21.02041296823865
4	14.34911590893038	16.754696447677873	31.396808953138855	37.4993786902529
5	12.072767797581209	41.635208773155234	32.2406129907366	14.05141043852696
6	32.048203172211366	32.110595751026146	17.859581381112708	17.981619695649787
7	25.644657913393342	34.02540699058487	22.152243124390136	18.177691971631653
8	29.209013831008978	31.743434390998726	20.990590100377496	18.0569616776148
9	26.923901982174296	13.05169035491829	22.523720952134298	37.50068671077312
10	18.610777565878454	29.853213937220247	29.977868292797776	21.55814020410352
11	30.249151748692633	24.413025791548616	21.193987291272627	24.14383516848612
12	20.15084092639245	30.481979401292847	28.692607329623787	20.67457234269091
13	28.093533931360316	20.849977894453207	29.7782643614113	21.278223812775174
14	22.596316091006834	23.807412290684017	28.819746924189747	24.776524694119402
15	25.972578657813983	27.031421268936867	27.375169061652237	19.62083101159691
16	22.159437237251378	27.225793118242443	29.21594633976618	21.398823304740006
17	20.12899698370468	29.98113834409834	29.168595996934	20.721268675262976
18	18.475135837931024	25.719345885098242	33.28872983359363	22.516788443377102
19	19.989038788040506	27.914727526245432	33.81612370734872	18.280109978365342
20	20.73840374407794	25.222952097672508	34.683079708154466	19.35556445009509
21	22.001820764564155	26.614685931192888	32.07985726880083	19.303636035442125
22	21.177375430665755	26.85457689460232	31.349197006202633	20.618850668529287
23	19.896169331104467	27.6476297360153	32.35545719241244	20.1007437404678
24	19.791658491538417	28.813991633900756	32.28194643917574	19.112403435385094
25	19.54064935370706	27.92179083705465	32.58736923064849	19.950190578589797
26	19.958561909919244	28.60026108089584	32.34263859131422	19.0985384178707
27	20.163136319282575	28.51864060043374	31.15613317741729	20.162089902866395
28	18.524186607439496	28.674425844392648	33.51514818564474	19.28623936252312
29	18.857470235993063	29.31038542132649	32.92954739873959	18.902596943940857
30	18.00294043012947	30.499506876263876	33.15897419798722	18.33857849561944
31	18.856423819576882	29.87767392094847	32.13924140041909	19.12666085905556
32	17.339120016114812	29.989248071323743	32.36670616888638	20.304925743675067
33	17.936231383597946	29.63503611444656	32.82647538174579	19.6022571202097
34	18.60946954535823	29.682909665486832	31.7814977881373	19.92612300101764
35	18.63026707162982	29.781534412711867	32.13570974501448	19.452488770643832
36	17.936754591806036	29.86812537115082	31.611062714351863	20.58405732269128
37	18.64583251582051	29.237136272193837	31.40714231524864	20.70988889673701
38	18.184101272180758	28.614780108670345	32.623993805214816	20.57712481393408
39	18.39547738824927	28.734202382166973	32.180836452962275	20.689483776621486
40	17.598762089379658	28.801042230750518	33.087425475530864	20.512770204338963
41	16.920291845538475	29.188085502685368	33.0077670258491	20.88385562592706
42	18.02779282001376	28.365078991359216	32.536225628307655	21.070902560319364
43	16.971827854035375	28.495096231069674	32.1861993370952	22.34687657779975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128.0
1	221.0
2	314.0
3	1162.0
4	2010.0
5	2010.0
6	3092.0
7	4174.0
8	4379.5
9	4585.0
10	6704.5
11	8824.0
12	8824.0
13	15067.0
14	21310.0
15	28320.5
16	35331.0
17	31795.5
18	28260.0
19	28260.0
20	29136.5
21	30013.0
22	23460.0
23	16907.0
24	15469.0
25	14031.0
26	14031.0
27	14766.0
28	15501.0
29	18161.5
30	20822.0
31	21189.0
32	21556.0
33	21556.0
34	22482.0
35	23408.0
36	24518.0
37	25628.0
38	31052.0
39	36476.0
40	36476.0
41	39480.0
42	42484.0
43	41345.5
44	40207.0
45	44043.5
46	47880.0
47	47880.0
48	49780.0
49	51680.0
50	51186.5
51	50693.0
52	52633.0
53	54573.0
54	54573.0
55	46250.5
56	37928.0
57	35385.0
58	32842.0
59	28642.5
60	24443.0
61	24443.0
62	22557.0
63	20671.0
64	18285.5
65	15900.0
66	13872.0
67	11844.0
68	11844.0
69	10152.5
70	8461.0
71	7497.5
72	6534.0
73	5083.0
74	3632.0
75	3632.0
76	2842.5
77	2053.0
78	1715.0
79	1377.0
80	1066.0
81	755.0
82	755.0
83	576.0
84	397.0
85	390.5
86	384.0
87	329.0
88	274.0
89	274.0
90	235.5
91	197.0
92	109.0
93	21.0
94	12.5
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	764514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.43821536155585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21584810858671	22.43658550584205
2	10.507222588341211	6.186277628147858
3	3.915264881561135	3.4577523234279948
4	2.018293211335367	2.3765980087222673
5	1.2152269163200768	1.7887055837894916
6	0.9051366404926896	1.5987364412675373
7	0.595655817340429	1.227453096256179
8	0.45781313501703147	1.0781761331184336
9	0.3867210356310651	1.0245939418576089
>10	2.9103572619352804	17.32022003555727
>50	0.456769187680241	9.548570797840156
>100	0.39158023224536265	21.10136222340617
>500	0.018306487482394378	3.63317565446916
>1k	0.004018497252232912	1.7454122323973795
>5k	8.929993893850917E-4	2.182455358590714
>10k+	8.929993893850917E-4	3.2939250353097624
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12728	1.6648485181435528	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12332	1.613050905542606	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9157	1.197754390370876	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7447	0.9740828814122436	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1959	0.256241219912258	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1947	0.2546715952879869	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1801	0.23557449569268843	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1523	0.19921152523040783	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	1453	0.19005538158882634	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1275	0.16677261632880497	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1242	0.16245614861205943	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1054	0.1378653628318121	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1025	0.13407210332315694	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	996	0.13027884381450178	TruSeq Adapter, Index 7 (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	990	0.1294940315023662	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	966	0.12635478225382402	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	906	0.11850665913246848	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	877	0.1147133996238133	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	831	0.10869650523077407	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	820	0.10725768265852556	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	789	0.10320281904582519	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	783	0.10241800673368964	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.924061560677764E-4	0.0
3	0.0	0.0	0.0	7.848123121355528E-4	0.0
4	0.0	0.0	0.0	0.0018312287283162897	0.0
5	0.0	0.0	0.0	0.001962030780338882	0.0
6	0.0	0.0	0.0	0.001962030780338882	0.0
7	0.0	0.0	0.0	0.0022236348843840664	0.0
8	0.0	0.0	0.0	0.0024852389884292506	0.0
9	0.0	0.0	0.0	0.0036624574566325794	0.0
10	0.0	0.0	0.0	0.006409300549107015	0.0
11	0.0	0.0	0.0	0.009548549797649226	0.0
12	0.0	0.0	0.0	0.014519027774507726	1.3080205202259214E-4
13	0.0	0.0	0.0	0.016350256502824016	1.3080205202259214E-4
14	0.0	0.0	0.0	0.020928328323614742	1.3080205202259214E-4
15	0.0	0.0	0.0	0.029168857601038045	1.3080205202259214E-4
16	0.0	0.0	0.0	0.0448651038437491	1.3080205202259214E-4
17	0.0	0.0	0.0	0.06958669167601901	1.3080205202259214E-4
18	0.0	0.0	0.0	0.07638839838119381	1.3080205202259214E-4
19	0.0	0.0	0.0	0.09208464462390487	1.3080205202259214E-4
20	0.0	0.0	0.0	0.10385682930593815	1.3080205202259214E-4
21	0.0	0.0	0.0	0.13250247869888582	1.3080205202259214E-4
22	0.0	0.0	0.0	0.1945026513575945	1.3080205202259214E-4
23	0.0	0.0	0.0	0.28017799543239236	1.3080205202259214E-4
24	0.0	0.0	0.0	0.4240602526572437	1.3080205202259214E-4
25	0.0	0.0	0.0	0.455714349246711	1.3080205202259214E-4
26	0.0	0.0	0.0	0.5113052213563126	1.3080205202259214E-4
27	0.0	0.0	0.0	0.5662420832058014	1.3080205202259214E-4
28	0.0	0.0	0.0	0.654533468321051	1.3080205202259214E-4
29	0.0	0.0	0.0	0.7776181992743102	1.3080205202259214E-4
30	0.0	0.0	0.0	0.9997200836086717	1.3080205202259214E-4
31	0.0	0.0	0.0	1.5603376785775016	1.3080205202259214E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCTCC	30	3.598361E-4	30.833334	8
AAGTCTC	30	3.598361E-4	30.833334	7
TCCTAGA	25	0.005495207	29.599998	1
GTACGAG	25	0.005495207	29.599998	3
GCAGCGT	95	1.0913936E-11	25.31579	1
AGCGTCA	90	1.4188117E-10	24.666668	3
AGTCGGT	275	0.0	23.545456	11
TGCGATA	40	0.0019305985	23.125002	10
CGATAAG	40	0.0019305985	23.125002	12
CAGTCGG	280	0.0	22.464287	10
GCCGTCT	175	0.0	22.2	36
TGCCGAT	60	3.7241854E-5	21.583334	12
GATACAC	95	7.1358954E-9	21.421053	3
GCAGTCG	305	0.0	20.62295	9
CCTAAAT	45	0.003824653	20.555557	24
CCTCGCC	270	0.0	20.555555	22
AGCGTCG	55	5.1409274E-4	20.181818	17
GCCTAAG	55	5.1409274E-4	20.181818	1
TCGGTGA	315	0.0	19.968254	13
CTATGCC	65	6.8982685E-5	19.923077	9
>>END_MODULE