FastQCFastQC Report
Fri 10 Feb 2017
ERR1630465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489265
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC45930.3084071672939336No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT38120.2559651908827509No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA34000.2283005375134714No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA32570.21869848549452248No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG27260.1830433133122715No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG27060.18170036897395694No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG26690.17921592194807506No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG25710.17263549469033385No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG25680.17243405303958664No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG24310.16323488432213204No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG21740.14597804957479024No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA20540.13792038354490302No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG20380.1368460280742514No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA20100.13496590600061104No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA18870.12670679831997664No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC17920.12032781271298258No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT17490.11744048238560632No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA17370.11663471578261761No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC16640.11173296894776955No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG16240.10904708027114046No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA15980.10730125263133156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15880.10662978046217428No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA15770.1058911610761013No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA15560.10448106952087105No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC15340.10300383074872503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.020.9850751
GCTTTAT8850.016.7231641
TTTATTG9700.016.211343
CGTACAC803.383405E-416.18753
TAATACT2300.016.0869564
TAGACTG1201.936336E-615.4166675
CTAGCGG2300.015.28260929
GCCTTAC855.365473E-415.2352941
TTAAGAC1101.4523621E-515.1363643
ACCTAGG14050.014.8790031
TCTAGCG2251.8189894E-1214.828
GCGACCT1252.9599832E-614.79999933
TCTTATA9150.014.759562537
GTCTAGA1551.2107193E-714.322581
CGCAATA2353.6379788E-1214.17021436
TTATTGA10600.014.1367924
GTATTAG4200.014.0952381
CTTTATT11250.013.9777782
CTCGTAT1203.3016455E-513.87500129
TACACTA800.006299773713.8750015
Produced by FastQC (version 0.11.5)