FastQCFastQC Report
Fri 10 Feb 2017
ERR1630464.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630464.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052013
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68700.33479320062787127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66170.3224638440399744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51610.251509127866149No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36030.17558368294937704No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG30830.15024271288729651No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT27000.13157811378387954No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA25830.12587639551991142No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG25060.12212398264533411No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA23190.11301097994993209No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC22670.11047688294372404No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC21560.10506756048816454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAA555.144986E-420.18181834
CGAATTA851.2462842E-619.58823615
CTATACG500.007036719518.54
GGTATCA32350.017.7851621
TCTAGCG2000.017.57528
CGCTAAG650.001580505717.07692335
CTAGCGG2200.016.81818229
TATACTG2650.014.66037755
TACGACG2850.014.2807025
CGAACGA1854.9039954E-914.016
CAATACG2009.840733E-1013.8750014
TAATACT3200.013.8754
TACACTC1753.5699486E-813.7428575
TTACACT2304.0017767E-1113.6739134
GTCTACG950.001245656913.6315792
CTTATAC10400.013.51923137
CGCAATA2601.8189894E-1213.51923136
CGAGCCG3150.013.50793715
TCTTATA15150.013.43234237
CGACGGT2900.013.3965517
Produced by FastQC (version 0.11.5)