##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630464.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2052013
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55154670072753	31.0	31.0	34.0	30.0	34.0
2	31.736739484593908	31.0	31.0	34.0	30.0	34.0
3	31.822987963526547	33.0	31.0	34.0	30.0	34.0
4	35.51030280997245	37.0	35.0	37.0	33.0	37.0
5	35.41843886953932	37.0	35.0	37.0	33.0	37.0
6	35.450383598934316	37.0	35.0	37.0	33.0	37.0
7	35.4428904690175	37.0	35.0	37.0	33.0	37.0
8	35.42067374816826	37.0	35.0	37.0	33.0	37.0
9	37.07278024067099	39.0	37.0	39.0	33.0	39.0
10	36.908945021303474	39.0	37.0	39.0	33.0	39.0
11	37.04732621089632	39.0	37.0	39.0	33.0	39.0
12	36.96494905246702	39.0	37.0	39.0	33.0	39.0
13	37.03063089756254	39.0	37.0	39.0	33.0	39.0
14	38.18790621696841	40.0	38.0	41.0	33.0	41.0
15	38.160961943223555	40.0	38.0	41.0	33.0	41.0
16	38.1372886039221	40.0	37.0	41.0	33.0	41.0
17	38.105217657003145	40.0	37.0	41.0	33.0	41.0
18	38.07771929320136	40.0	37.0	41.0	33.0	41.0
19	38.120154209549355	40.0	37.0	41.0	33.0	41.0
20	38.086997499528515	40.0	37.0	41.0	33.0	41.0
21	38.01482105620189	40.0	37.0	41.0	33.0	41.0
22	37.961528021508634	40.0	37.0	41.0	33.0	41.0
23	37.932453156973175	40.0	37.0	41.0	33.0	41.0
24	37.878409152378666	40.0	37.0	41.0	33.0	41.0
25	37.82080669079582	40.0	37.0	41.0	33.0	41.0
26	37.59477839565344	39.0	37.0	41.0	32.0	41.0
27	37.470413686463	39.0	37.0	41.0	32.0	41.0
28	37.381658888125955	39.0	36.0	41.0	32.0	41.0
29	37.26946028119705	39.0	36.0	40.0	31.0	41.0
30	37.18635846848923	39.0	36.0	40.0	31.0	41.0
31	37.13417215193081	39.0	36.0	40.0	31.0	41.0
32	37.030795613867944	39.0	36.0	40.0	31.0	41.0
33	37.00334208409011	39.0	36.0	40.0	31.0	41.0
34	36.92331871191849	39.0	36.0	40.0	31.0	41.0
35	36.87628587148327	39.0	36.0	40.0	31.0	41.0
36	36.84075247086641	39.0	36.0	40.0	30.0	41.0
37	36.643952548058905	39.0	35.0	40.0	30.0	41.0
38	36.51740754079044	39.0	35.0	40.0	30.0	41.0
39	36.459481007186604	39.0	35.0	40.0	30.0	41.0
40	36.36607370421142	39.0	35.0	40.0	30.0	41.0
41	36.231153506337435	39.0	35.0	40.0	30.0	41.0
42	36.158186132349066	38.0	35.0	40.0	29.0	41.0
43	35.27127167323014	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	3.0
12	5.0
13	0.0
14	7.0
15	8.0
16	23.0
17	60.0
18	116.0
19	251.0
20	474.0
21	941.0
22	1647.0
23	2837.0
24	4736.0
25	7181.0
26	10653.0
27	15698.0
28	21967.0
29	30013.0
30	39916.0
31	51144.0
32	65502.0
33	83385.0
34	108287.0
35	144468.0
36	204238.0
37	318624.0
38	547684.0
39	392140.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.07609795844373	20.411761523927968	13.10415674754497	24.407983770083327
2	18.005733881802893	22.293523481576383	35.77891563065146	23.92182700596926
3	20.126431947555886	24.528597041051885	30.625000913736898	24.719970097655327
4	14.879925224645262	16.34560794692821	34.891884213209174	33.88258261521735
5	13.5347583080614	38.22042063086345	34.78160226080439	13.46321880027076
6	33.678928934660746	35.60757168692401	16.212811517275963	14.50068786113928
7	27.65840177425777	31.61461452729588	22.17037611360162	18.556607584844738
8	25.802419380384045	35.144416726404756	20.760540990724717	18.292622902486485
9	26.007778703156365	14.664770642291252	20.54631232843067	38.78113832612172
10	16.342683988844126	27.962834543445876	33.77688153047763	21.917599937232364
11	34.19047540147162	22.659700498973447	22.43499432021142	20.714829779343503
12	20.488564156269966	26.662452918183266	29.973104458889882	22.87587846665689
13	28.806347718070015	20.715268373056116	26.7404251337589	23.737958775114972
14	22.222130171689948	21.994597500113304	26.043499724416954	29.7397726037798
15	24.748527421609904	28.759125795012018	23.676068329001815	22.816278454376263
16	24.294144335342903	27.718440380251003	24.88458893778938	23.102826346616713
17	22.843666195097203	27.7817928054062	26.246617345991474	23.12792365350512
18	21.8666256012998	26.30592496246369	28.393046242884427	23.43440319335209
19	23.313546259209858	26.646907207702874	28.269606479101256	21.769940053986012
20	23.679625811337452	25.611679848032153	28.314586700961446	22.39410763966895
21	23.451362150239788	26.79724738585964	26.87249057389013	22.878899890010445
22	23.363984536160345	26.29749421665457	27.136962582595725	23.201558664589356
23	22.68801415975435	26.756896764299253	27.45689232962949	23.09819674631691
24	23.715298099963306	26.879118212214053	27.16045171253788	22.245131975284757
25	23.514860773299194	26.063577569927677	27.53033241017479	22.89122924659834
26	23.28035933495548	26.777559401426792	27.25362851015076	22.68845275346696
27	23.635766440076157	26.12678379717867	26.868591963111342	23.368857799633822
28	22.569398926809917	26.62151750500606	27.78861537426907	23.02046819391495
29	22.59147481034477	27.129896350559186	27.523119980234046	22.755508858862004
30	22.223884546540397	27.851236809903252	27.544611072152076	22.380267571404275
31	23.118566987636044	26.954897459226622	27.284476267937873	22.64205928519946
32	22.25478103696224	26.924585760421593	27.31040202961677	23.51023117299939
33	22.476514525005445	26.595591743327162	27.757085359595674	23.170808372071715
34	22.86764265138671	26.44398451666729	27.602797838025396	23.085574993920606
35	23.16676356338873	26.550172927754357	27.622778218266646	22.660285290590267
36	22.53509115195664	27.044419309234396	27.521511803287797	22.89897773552117
37	23.474071558026193	25.922252929196844	27.260109950570488	23.34356556220648
38	22.622322568131878	26.32463829420184	28.177745462626213	22.87529367504007
39	22.763354813054303	26.117963190291682	28.083399081779696	23.035282914874323
40	22.332753252537874	25.723375046844243	28.890070384544348	23.053801316073532
41	21.66048655637172	26.373858255283956	28.966288225269526	22.999366963074795
42	22.60702052082516	25.53638792736693	28.640656759971794	23.215934791836116
43	21.727493929132027	25.65047102528103	28.72082194411049	23.901213101476454
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	433.0
1	393.5
2	354.0
3	785.0
4	1216.0
5	1216.0
6	1717.5
7	2219.0
8	2277.0
9	2335.0
10	3500.0
11	4665.0
12	4665.0
13	7482.0
14	10299.0
15	15103.0
16	19907.0
17	20022.5
18	20138.0
19	20138.0
20	22627.0
21	25116.0
22	26661.5
23	28207.0
24	31176.5
25	34146.0
26	34146.0
27	38200.0
28	42254.0
29	54347.5
30	66441.0
31	70456.0
32	74471.0
33	74471.0
34	80714.5
35	86958.0
36	92372.0
37	97786.0
38	110785.0
39	123784.0
40	123784.0
41	130989.0
42	138194.0
43	131019.5
44	123845.0
45	129840.0
46	135835.0
47	135835.0
48	139732.0
49	143629.0
50	146758.0
51	149887.0
52	156931.0
53	163975.0
54	163975.0
55	144181.5
56	124388.0
57	115905.0
58	107422.0
59	95941.5
60	84461.0
61	84461.0
62	77999.0
63	71537.0
64	62479.0
65	53421.0
66	46155.0
67	38889.0
68	38889.0
69	33141.0
70	27393.0
71	23849.5
72	20306.0
73	16086.0
74	11866.0
75	11866.0
76	9150.0
77	6434.0
78	5317.0
79	4200.0
80	3270.5
81	2341.0
82	2341.0
83	1791.5
84	1242.0
85	1056.0
86	870.0
87	770.5
88	671.0
89	671.0
90	533.0
91	395.0
92	226.5
93	58.0
94	39.5
95	21.0
96	21.0
97	12.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2052013.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.78399296116051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.97217992760528	46.777111130431074
2	8.220482005505586	8.842606926429182
3	1.9297280317795158	3.113654366345508
4	0.804867030367348	1.731558507837904
5	0.4383809860828759	1.1788939934894
6	0.2770930449365228	0.8941902227071487
7	0.1871145203013847	0.704463622997442
8	0.13659814893962755	0.5877435104861187
9	0.1087065775735322	0.5262006422742036
>10	0.684755600751718	7.320439613211237
>50	0.09611101549570739	3.6901908118441233
>100	0.12307764984042155	14.15417287738116
>500	0.016269902116830014	5.878254236037415
>1k	0.004362878779932071	3.688892471949929
>5k	2.7267992374575443E-4	0.911627066578172
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6870	0.33479320062787127	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6617	0.3224638440399744	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5161	0.251509127866149	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3603	0.17558368294937704	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3083	0.15024271288729651	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2700	0.13157811378387954	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2583	0.12587639551991142	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2506	0.12212398264533411	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2319	0.11301097994993209	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2267	0.11047688294372404	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2156	0.10506756048816454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.385937126129318E-4	0.0
3	0.0	0.0	0.0	7.30989521021553E-4	0.0
4	0.0	0.0	0.0	0.0010721179641649443	0.0
5	0.0	0.0	0.0	0.001218315868369255	0.0
6	0.0	0.0	0.0	0.0012670485031040252	0.0
7	0.0	0.0	0.0	0.0015107116767778762	0.0
8	0.0	0.0	0.0	0.001705642215716957	0.0
9	0.0	0.0	0.0	0.002972690718820982	0.0
10	0.0	0.0	0.0	0.004483402395598858	0.0
11	0.0	4.87326347347702E-5	0.0	0.005165659281885641	0.0
12	0.0	4.87326347347702E-5	0.0	0.005750450898702884	0.0
13	0.0	4.87326347347702E-5	0.0	0.006189044611315815	0.0
14	0.0	4.87326347347702E-5	0.0	0.0065789056891939765	0.0
15	0.0	4.87326347347702E-5	0.0	0.00730989521021553	0.0
16	0.0	4.87326347347702E-5	0.0	0.008479478443850014	0.0
17	0.0	4.87326347347702E-5	0.0	0.010477516467975593	0.0
18	0.0	4.87326347347702E-5	0.0	0.011257238623731916	0.0
19	0.0	9.74652694695404E-5	0.0	0.01237808922263163	0.0
20	0.0	9.74652694695404E-5	0.0	0.013645137725735655	0.0
21	0.0	9.74652694695404E-5	0.0	0.016179234731943708	0.0
22	0.0	9.74652694695404E-5	0.0	0.020175310780194862	0.0
23	0.0	9.74652694695404E-5	0.0	0.026218157487306368	0.0
24	0.0	9.74652694695404E-5	0.0	0.035769753895321325	0.0
25	0.0	9.74652694695404E-5	0.0	0.03854751407520323	0.0
26	0.0	9.74652694695404E-5	0.0	0.043323312279210706	0.0
27	0.0	9.74652694695404E-5	0.0	0.05253378024408228	0.0
28	0.0	9.74652694695404E-5	0.0	0.07475586168313748	0.0
29	0.0	9.74652694695404E-5	0.0	0.10906363653641571	0.0
30	0.0	9.74652694695404E-5	0.0	0.16018417037318963	0.0
31	0.0	9.74652694695404E-5	0.0	0.35068003955140636	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTAA	55	5.144986E-4	20.181818	34
CGAATTA	85	1.2462842E-6	19.588236	15
CTATACG	50	0.0070367195	18.5	4
GGTATCA	3235	0.0	17.785162	1
TCTAGCG	200	0.0	17.575	28
CGCTAAG	65	0.0015805057	17.076923	35
CTAGCGG	220	0.0	16.818182	29
TATACTG	265	0.0	14.6603775	5
TACGACG	285	0.0	14.280702	5
CGAACGA	185	4.9039954E-9	14.0	16
CAATACG	200	9.840733E-10	13.875001	4
TAATACT	320	0.0	13.875	4
TACACTC	175	3.5699486E-8	13.742857	5
TTACACT	230	4.0017767E-11	13.673913	4
GTCTACG	95	0.0012456569	13.631579	2
CTTATAC	1040	0.0	13.519231	37
CGCAATA	260	1.8189894E-12	13.519231	36
CGAGCCG	315	0.0	13.507937	15
TCTTATA	1515	0.0	13.432342	37
CGACGGT	290	0.0	13.396551	7
>>END_MODULE