FastQCFastQC Report
Fri 10 Feb 2017
ERR1630463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136000
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43073.166911764705883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34392.5286764705882354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27382.013235294117647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19411.4272058823529412No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT9120.6705882352941176No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT7230.5316176470588235TruSeq Adapter, Index 1 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.40220588235294114No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC4940.36323529411764705No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.3514705882352941No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC4650.34191176470588236No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.3330882352941177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4260.31323529411764706No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG4200.30882352941176466No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3900.2867647058823529No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3660.2691176470588236No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.2580882352941176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2780.20441176470588235No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2740.2014705882352941No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.1948529411764706No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2120.15588235294117647No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.15588235294117647No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1990.1463235294117647No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.13308823529411765No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.13308823529411765No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1780.13088235294117645No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1780.13088235294117645No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1780.13088235294117645No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.12352941176470589No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA1570.11544117647058823No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1550.11397058823529412No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1530.11249999999999999No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1440.10588235294117646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT1430.10514705882352941No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG1430.10514705882352941No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1410.10367647058823529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1380.10147058823529412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCC451.0680924E-732.8888937
TGCTAAT303.5829615E-430.83333236
GAAGACC250.00547953129.5999984
CGTGCTA250.00547953129.59999834
AGGACGT250.00547953129.59999830
GCTAATC250.00547953129.59999837
CCGCTTA556.193768E-726.9090925
TCCGCTT701.897115E-723.78571324
CTCCGCT701.897115E-723.78571323
TCCTCCG701.897115E-723.78571321
CCGTCTT1400.023.78571337
GCCGTCT1400.023.78571336
TGGGCAG3900.022.2948726
TGATTCC3600.022.09722317
GATTCCT3600.022.09722318
GCAGTCG3950.022.0126579
CTCGCCT2700.021.92592623
AGTCGGT3900.021.82051311
GTGATTC3650.021.7945216
GGGCAGT4000.021.73757
Produced by FastQC (version 0.11.5)