##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630463.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 136000 Sequences flagged as poor quality 0 Sequence length 43 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.589139705882353 31.0 31.0 34.0 30.0 34.0 2 31.6655 31.0 31.0 34.0 30.0 34.0 3 31.604176470588236 31.0 31.0 34.0 28.0 34.0 4 35.37694852941176 37.0 35.0 37.0 33.0 37.0 5 35.41319117647059 37.0 35.0 37.0 33.0 37.0 6 35.514889705882354 37.0 35.0 37.0 33.0 37.0 7 35.46413235294118 37.0 35.0 37.0 33.0 37.0 8 35.52607352941177 37.0 35.0 37.0 33.0 37.0 9 37.05519852941176 39.0 37.0 39.0 33.0 39.0 10 36.97385294117647 39.0 37.0 39.0 33.0 39.0 11 37.14910294117647 39.0 37.0 39.0 33.0 39.0 12 37.105904411764705 39.0 37.0 39.0 33.0 39.0 13 37.16821323529412 39.0 37.0 39.0 34.0 39.0 14 38.146301470588234 40.0 38.0 41.0 33.0 41.0 15 38.08518382352941 40.0 37.0 41.0 33.0 41.0 16 37.90684558823529 40.0 37.0 41.0 33.0 41.0 17 38.06718382352941 40.0 37.0 41.0 33.0 41.0 18 38.1829044117647 40.0 38.0 41.0 33.0 41.0 19 38.25161029411765 40.0 38.0 41.0 34.0 41.0 20 38.27943382352941 40.0 38.0 41.0 34.0 41.0 21 38.229323529411765 40.0 38.0 41.0 34.0 41.0 22 38.10066911764706 40.0 37.0 41.0 33.0 41.0 23 38.00391911764706 40.0 37.0 41.0 33.0 41.0 24 37.884375 40.0 37.0 41.0 33.0 41.0 25 37.81228676470588 40.0 37.0 41.0 33.0 41.0 26 37.43026470588235 39.0 36.0 41.0 32.0 41.0 27 37.15613235294118 39.0 35.0 41.0 32.0 41.0 28 36.98664705882353 39.0 35.0 41.0 31.0 41.0 29 36.70993382352941 39.0 35.0 40.0 31.0 41.0 30 36.442404411764706 38.0 35.0 40.0 31.0 41.0 31 36.134 38.0 35.0 40.0 30.0 41.0 32 35.783382352941175 38.0 35.0 40.0 30.0 41.0 33 35.53038970588235 38.0 35.0 40.0 29.0 41.0 34 35.26422794117647 37.0 35.0 40.0 28.0 41.0 35 34.982058823529414 37.0 34.0 40.0 26.0 41.0 36 34.70497058823529 37.0 34.0 40.0 24.0 41.0 37 34.20266911764706 37.0 33.0 40.0 22.0 41.0 38 33.834904411764704 36.0 33.0 40.0 21.0 41.0 39 33.428264705882356 36.0 33.0 40.0 18.0 41.0 40 32.99008823529412 35.0 33.0 40.0 15.0 41.0 41 32.49469117647059 35.0 32.0 40.0 12.0 41.0 42 32.04491911764706 35.0 31.0 40.0 10.0 41.0 43 31.04436029411765 35.0 29.0 39.0 8.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 1.0 15 2.0 16 1.0 17 6.0 18 20.0 19 34.0 20 67.0 21 120.0 22 217.0 23 398.0 24 569.0 25 914.0 26 1329.0 27 1941.0 28 2483.0 29 3227.0 30 4058.0 31 4752.0 32 5485.0 33 7024.0 34 8656.0 35 10368.0 36 14802.0 37 22941.0 38 26934.0 39 19650.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.59117647058824 19.011764705882353 14.326470588235294 19.070588235294117 2 19.215441176470588 22.923529411764708 32.495588235294115 25.365441176470586 3 24.352941176470587 25.70514705882353 30.675735294117644 19.266176470588235 4 14.28529411764706 18.21985294117647 29.38676470588235 38.10808823529412 5 12.230882352941176 40.85441176470589 31.001470588235293 15.913235294117648 6 30.820588235294117 29.02794117647059 18.350735294117648 21.800735294117647 7 24.616176470588236 35.3389705882353 20.906617647058823 19.13823529411765 8 33.38235294117647 28.817647058823532 19.399264705882352 18.40073529411765 9 27.93455882352941 10.969117647058823 22.26544117647059 38.830882352941174 10 21.644117647058824 28.778676470588234 25.520588235294117 24.05661764705882 11 28.72941176470588 23.607352941176472 18.97279411764706 28.69044117647059 12 21.243382352941175 31.216911764705884 27.485294117647058 20.054411764705883 13 32.46176470588235 17.68529411764706 29.820588235294114 20.03235294117647 14 23.751470588235293 22.906617647058823 30.025000000000002 23.316911764705882 15 30.88823529411765 21.41470588235294 27.62279411764706 20.074264705882353 16 22.154411764705884 23.249264705882354 32.677205882352936 21.919117647058822 17 18.626470588235293 29.701470588235296 30.33235294117647 21.33970588235294 18 19.42058823529412 20.201470588235292 34.989705882352936 25.388235294117646 19 19.449264705882353 26.094852941176473 37.22794117647059 17.227941176470587 20 20.70735294117647 20.897058823529413 38.85514705882353 19.540441176470587 21 24.83970588235294 20.45735294117647 36.14926470588235 18.553676470588236 22 23.141911764705885 22.56544117647059 34.73529411764706 19.55735294117647 23 21.298529411764704 22.661764705882355 35.00955882352941 21.030147058823527 24 19.713970588235295 25.076470588235296 36.65294117647059 18.55661764705882 25 18.645588235294117 24.72279411764706 36.16102941176471 20.47058823529412 26 21.777941176470588 23.383088235294117 35.66838235294117 19.17058823529412 27 18.861764705882354 25.55 35.53235294117647 20.055882352941175 28 18.94485294117647 24.206617647058824 37.038970588235294 19.80955882352941 29 18.121323529411764 25.572058823529414 37.69558823529412 18.611029411764708 30 17.308823529411764 26.645588235294117 38.25514705882353 17.79044117647059 31 18.655882352941177 26.31323529411765 35.39191176470588 19.638970588235296 32 17.23455882352941 27.181617647058825 37.30882352941177 18.275 33 17.305882352941175 25.85 37.63308823529412 19.211029411764706 34 17.7 28.024264705882352 34.558088235294115 19.71764705882353 35 17.08235294117647 27.3625 36.60588235294118 18.949264705882353 36 17.55735294117647 27.811764705882354 33.91029411764706 20.72058823529412 37 16.698529411764707 28.22279411764706 34.739705882352936 20.338970588235295 38 17.197794117647057 26.090441176470584 35.58308823529412 21.128676470588236 39 18.01544117647059 26.205882352941178 35.06838235294117 20.71029411764706 40 16.858088235294115 26.181617647058825 35.99632352941177 20.963970588235295 41 16.08529411764706 27.434558823529407 34.70220588235294 21.777941176470588 42 16.53529411764706 26.372794117647057 35.595588235294116 21.496323529411764 43 15.386029411764707 27.913970588235294 35.05220588235294 21.64779411764706 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10.0 1 35.0 2 60.0 3 219.0 4 378.0 5 378.0 6 662.5 7 947.0 8 989.0 9 1031.0 10 1390.0 11 1749.0 12 1749.0 13 3189.0 14 4629.0 15 6503.0 16 8377.0 17 7436.0 18 6495.0 19 6495.0 20 7184.0 21 7873.0 22 5442.5 23 3012.0 24 2598.5 25 2185.0 26 2185.0 27 2277.0 28 2369.0 29 2359.0 30 2349.0 31 2387.5 32 2426.0 33 2426.0 34 2501.5 35 2577.0 36 2601.5 37 2626.0 38 2937.5 39 3249.0 40 3249.0 41 3719.5 42 4190.0 43 4813.5 44 5437.0 45 6662.5 46 7888.0 47 7888.0 48 9554.0 49 11220.0 50 10927.0 51 10634.0 52 8533.5 53 6433.0 54 6433.0 55 6685.0 56 6937.0 57 6558.0 58 6179.0 59 5524.0 60 4869.0 61 4869.0 62 4614.0 63 4359.0 64 4149.5 65 3940.0 66 3617.0 67 3294.0 68 3294.0 69 3024.5 70 2755.0 71 2481.5 72 2208.0 73 1842.0 74 1476.0 75 1476.0 76 1186.0 77 896.0 78 709.5 79 523.0 80 387.5 81 252.0 82 252.0 83 182.0 84 112.0 85 72.0 86 32.0 87 21.5 88 11.0 89 11.0 90 9.5 91 8.0 92 5.5 93 3.0 94 2.5 95 2.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 136000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.999264705882354 #Duplication Level Percentage of deduplicated Percentage of total 1 85.04525461570341 46.77426470588235 2 8.498776721613925 9.348529411764705 3 2.4920119252931188 4.1117647058823525 4 1.0909236754502065 2.4 5 0.6377090602815546 1.7536764705882353 6 0.4024117969491571 1.3279411764705882 7 0.3101645743927058 1.1941176470588235 8 0.21390660302945227 0.9411764705882352 9 0.15240845465848474 0.7544117647058823 >10 1.0040241179694915 10.65 >50 0.08422572494284683 3.130147058823529 >100 0.05882431583309937 6.873529411764706 >500 0.00401074880680223 1.6044117647058824 >1k 0.005347665075736307 9.136029411764705 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4307 3.166911764705883 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 3439 2.5286764705882354 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2738 2.013235294117647 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1941 1.4272058823529412 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT 912 0.6705882352941176 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 723 0.5316176470588235 TruSeq Adapter, Index 1 (95% over 21bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.40220588235294114 No Hit CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC 494 0.36323529411764705 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.3514705882352941 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC 465 0.34191176470588236 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 453 0.3330882352941177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 426 0.31323529411764706 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG 420 0.30882352941176466 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 390 0.2867647058823529 No Hit CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA 366 0.2691176470588236 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 351 0.2580882352941176 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 278 0.20441176470588235 No Hit GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 274 0.2014705882352941 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 265 0.1948529411764706 No Hit GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 212 0.15588235294117647 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 212 0.15588235294117647 No Hit CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA 199 0.1463235294117647 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 181 0.13308823529411765 No Hit ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 181 0.13308823529411765 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 178 0.13088235294117645 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 178 0.13088235294117645 No Hit GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA 178 0.13088235294117645 No Hit GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 168 0.12352941176470589 No Hit CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA 157 0.11544117647058823 No Hit CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA 155 0.11397058823529412 No Hit GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA 153 0.11249999999999999 No Hit ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG 144 0.10588235294117646 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 143 0.10514705882352941 No Hit CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG 143 0.10514705882352941 No Hit CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA 141 0.10367647058823529 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 138 0.10147058823529412 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.352941176470588E-4 0.0 3 0.0 0.0 0.0 0.0029411764705882353 0.0 4 0.0 0.0 0.0 0.0058823529411764705 0.0 5 0.0 0.0 0.0 0.006617647058823529 0.0 6 0.0 0.0 0.0 0.006617647058823529 0.0 7 0.0 0.0 0.0 0.008088235294117648 0.0 8 0.0 0.0 0.0 0.009558823529411765 0.0 9 0.0 0.0 0.0 0.0125 0.0 10 0.0 0.0 0.0 0.015441176470588236 0.0 11 0.0 0.0 0.0 0.019852941176470587 0.0 12 0.0 0.0 0.0 0.022794117647058822 0.0 13 0.0 0.0 0.0 0.025735294117647058 0.0 14 0.0 0.0 0.0 0.030147058823529412 0.0 15 0.0 0.0 0.0 0.03897058823529412 0.0 16 0.0 0.0 0.0 0.06102941176470588 0.0 17 0.0 0.0 0.0 0.09117647058823529 0.0 18 0.0 0.0 0.0 0.10661764705882353 0.0 19 0.0 0.0 0.0 0.13455882352941176 0.0 20 7.352941176470588E-4 0.0 0.0 0.1463235294117647 0.0 21 7.352941176470588E-4 0.0 0.0 0.17941176470588235 0.0 22 7.352941176470588E-4 0.0 0.0 0.23602941176470588 0.0 23 7.352941176470588E-4 0.0 0.0 0.31691176470588234 0.0 24 7.352941176470588E-4 0.0 0.0 0.4823529411764706 0.0 25 7.352941176470588E-4 0.0 0.0 0.5169117647058824 0.0 26 7.352941176470588E-4 0.0 0.0 0.5882352941176471 0.0 27 7.352941176470588E-4 0.0 0.0 0.6441176470588236 0.0 28 7.352941176470588E-4 0.0 0.0 0.7110294117647059 0.0 29 7.352941176470588E-4 0.0 0.0 0.7970588235294118 0.0 30 7.352941176470588E-4 0.0 0.0 1.000735294117647 0.0 31 7.352941176470588E-4 0.0 0.0 1.5595588235294118 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGCCCC 45 1.0680924E-7 32.88889 37 TGCTAAT 30 3.5829615E-4 30.833332 36 GAAGACC 25 0.005479531 29.599998 4 CGTGCTA 25 0.005479531 29.599998 34 AGGACGT 25 0.005479531 29.599998 30 GCTAATC 25 0.005479531 29.599998 37 CCGCTTA 55 6.193768E-7 26.90909 25 TCCGCTT 70 1.897115E-7 23.785713 24 CTCCGCT 70 1.897115E-7 23.785713 23 TCCTCCG 70 1.897115E-7 23.785713 21 CCGTCTT 140 0.0 23.785713 37 GCCGTCT 140 0.0 23.785713 36 TGGGCAG 390 0.0 22.294872 6 TGATTCC 360 0.0 22.097223 17 GATTCCT 360 0.0 22.097223 18 GCAGTCG 395 0.0 22.012657 9 CTCGCCT 270 0.0 21.925926 23 AGTCGGT 390 0.0 21.820513 11 GTGATTC 365 0.0 21.79452 16 GGGCAGT 400 0.0 21.7375 7 >>END_MODULE