FastQCFastQC Report
Fri 10 Feb 2017
ERR1630462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457235
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78530.319586852702326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75030.30534320079276095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58160.23668879858865757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38200.15545928655582392No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA33790.137512285149772No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC30040.1222512295323809No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT26630.10837384295763328No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG26110.10625764324535504No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG25930.10552511257572027No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC25740.10475188575777246No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA25510.10381587434657247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA4650.021.4838711
CTAGAAC4900.019.6326523
TCTAGAC4850.019.4536083
GGTATCA35350.019.2065071
CTAGACA5450.018.6697254
GACGGAC6200.017.9032277
GCGTTAT5500.017.8272721
GTTCTAG5450.017.6513771
AAGACGG6450.017.4961225
CAAGACG7000.017.4428564
ACGGACC6650.017.2481218
ACAACGG4450.017.04494323
CTTATAC15500.016.70967737
CGCAAGA6950.016.2374112
CGTTATT5450.015.9541282
AGACGGA7350.015.8571436
CGAATTA1302.59226E-715.65384615
ACTGATC6050.015.5950418
TAGAACT6700.015.4626864
ACGGAGT5100.015.23529426
Produced by FastQC (version 0.11.5)