FastQCFastQC Report
Fri 10 Feb 2017
ERR1630461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104508
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28822.757683622306426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26842.5682244421479696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18581.7778543269414784No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT15861.5175871703601638No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT9850.9425115780610097RNA PCR Primer, Index 46 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.8870134343782294No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG7480.7157346805986144No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC7410.7090366287748306No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC5600.535844145902706No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3540.3387300493742106No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA3350.32054962299536877No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3280.31385157117158496No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.3100241129865656No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3090.29567114479274315No Hit
ACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA2640.25261224021127565No Hit
GGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2640.25261224021127565No Hit
TATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA2570.24591418838749188No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2490.23825927201745323No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA2430.2325180847399242No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.22486316836988554No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2160.20668274199104375No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.20285528380602444No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2050.19615723198224058No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.1942435028897309No Hit
TATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAAAA1980.18945918015845678No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1880.17989053469590843No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA1880.17989053469590843No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG1870.1789336701496536No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC1800.17223561832586978RNA PCR Primer, Index 46 (95% over 22bp)
GTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAA1720.16458070195583113No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1650.15788265013204733No Hit
GGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA1590.15214146285451832No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1510.14448654648447967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1510.14448654648447967No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1490.14257281739196997No Hit
ACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA1450.13874535920695066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC1420.13587476556818617No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.13300417192942168No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1380.13204730738316683No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA1340.12821984919814752No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.1215217973743637No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1250.11960806828185402No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1230.11769433918934437No Hit
GTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAAA1220.11673747464308953No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA1220.11673747464308953No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1210.11578061009683469No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1200.11482374555057985No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA1170.11195315191181536No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1080.10334137099552188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGC250.005473789329.626
ATGCCCC250.005473789329.637
CGTCTTC405.8893933E-527.7537
CCGTCTT1450.026.79310237
GCCGTCT1550.023.87096636
TACGGGA450.003802927220.5555552
GGCCTGT450.003802927220.55555534
AGTCAGA500.006993211318.515
GTCAGAG500.006993211318.516
TCAGAGG500.006993211318.517
GTTCCCA609.160449E-418.56
TGGATGC500.006993211318.52
TATACAC2500.018.53
TGCCGTC2000.018.535
ATGCCGT2100.017.6190534
TATGCCG2250.016.44444333
GGTATCA16000.016.418751
TTATACA2950.016.3050842
AGTCGGT1606.039045E-1016.187511
GGAACAG1403.369496E-815.85714322
Produced by FastQC (version 0.11.5)