##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630461.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104508
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.831917173804875	33.0	31.0	34.0	30.0	34.0
2	31.920599379951774	33.0	31.0	34.0	30.0	34.0
3	31.883023309220345	33.0	31.0	34.0	30.0	34.0
4	35.60560913997015	37.0	35.0	37.0	33.0	37.0
5	35.644888429593905	37.0	35.0	37.0	33.0	37.0
6	35.744507597504494	37.0	35.0	37.0	35.0	37.0
7	35.703563363570254	37.0	35.0	37.0	33.0	37.0
8	35.77196960998201	37.0	35.0	37.0	35.0	37.0
9	37.36090060091094	39.0	37.0	39.0	34.0	39.0
10	37.29462816243733	39.0	37.0	39.0	34.0	39.0
11	37.44449228767176	39.0	37.0	39.0	35.0	39.0
12	37.37540666743216	39.0	37.0	39.0	34.0	39.0
13	37.41962337811459	39.0	37.0	39.0	34.0	39.0
14	38.42593868411988	40.0	38.0	41.0	34.0	41.0
15	38.37372258583075	40.0	38.0	41.0	34.0	41.0
16	38.22213610441306	40.0	37.0	41.0	33.0	41.0
17	38.39136716806369	40.0	38.0	41.0	34.0	41.0
18	38.54560416427451	40.0	38.0	41.0	34.0	41.0
19	38.60615455276151	40.0	38.0	41.0	34.0	41.0
20	38.633157270256824	40.0	38.0	41.0	34.0	41.0
21	38.57443449305316	40.0	38.0	41.0	34.0	41.0
22	38.450070807976424	40.0	38.0	41.0	34.0	41.0
23	38.402849542618746	40.0	38.0	41.0	34.0	41.0
24	38.257377425651626	40.0	38.0	41.0	34.0	41.0
25	38.12797106441612	40.0	37.0	41.0	33.0	41.0
26	37.79397749454587	40.0	37.0	41.0	33.0	41.0
27	37.566607341064795	40.0	36.0	41.0	33.0	41.0
28	37.42540283997398	39.0	35.0	41.0	33.0	41.0
29	37.20574501473571	39.0	35.0	41.0	32.0	41.0
30	36.90925096643319	39.0	35.0	41.0	31.0	41.0
31	36.58180235005933	38.0	35.0	40.0	31.0	41.0
32	36.30283806024419	38.0	35.0	40.0	31.0	41.0
33	36.055412025873615	38.0	35.0	40.0	30.0	41.0
34	35.785872851839095	38.0	35.0	40.0	30.0	41.0
35	35.52017070463505	38.0	35.0	40.0	29.0	41.0
36	35.20449152218012	37.0	35.0	40.0	27.0	41.0
37	34.752018984192595	37.0	34.0	40.0	24.0	41.0
38	34.40064875416236	37.0	33.0	40.0	22.0	41.0
39	34.013376966356645	36.0	33.0	40.0	21.0	41.0
40	33.56634898763731	36.0	33.0	40.0	18.0	41.0
41	33.15777739503196	35.0	33.0	40.0	15.0	41.0
42	32.719227236192445	35.0	33.0	40.0	12.0	41.0
43	31.67505836873732	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	1.0
13	2.0
14	3.0
15	1.0
16	1.0
17	2.0
18	3.0
19	15.0
20	33.0
21	59.0
22	129.0
23	191.0
24	331.0
25	516.0
26	784.0
27	1170.0
28	1603.0
29	2135.0
30	2677.0
31	3220.0
32	3978.0
33	4976.0
34	5958.0
35	7734.0
36	10626.0
37	17387.0
38	19928.0
39	21037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.43567956520075	18.67321162016305	15.117502966280094	19.77360584835611
2	18.384238527194093	22.54277184521759	34.1284877712711	24.94450185631722
3	22.479618785164774	25.747311210625025	32.64056340184484	19.132506602365368
4	12.40192138400888	18.852145290312702	28.40930837830597	40.33662494737245
5	11.178091629348948	42.0388869751598	31.148811574233555	15.634209821257702
6	29.54893405289547	30.155586175221032	18.46557201362575	21.82990775825774
7	24.086194358326633	35.67860833620393	19.038733876832396	21.196463428637042
8	34.46051976882153	28.570061622076782	19.105714395070233	17.86370421403146
9	27.369196616526963	10.358058713208559	20.634783939985454	41.63796073027902
10	22.284418417728784	29.383396486393387	23.945535270027175	24.386649825850654
11	30.172809737053623	22.793470356336357	18.169900868833007	28.86381903777701
12	21.209859532284607	32.914226662073716	25.222949439277375	20.652964366364298
13	34.36770390783481	17.840739464921345	28.53274390477284	19.258812722471006
14	23.80391931718146	23.682397519807097	28.750909021318943	23.762774141692503
15	31.413863053546144	23.32931450223906	24.94450185631722	20.312320587897577
16	23.512075630573737	23.460404945075975	30.10582921881578	22.921690205534503
17	17.92207295135301	32.53243770811804	27.798828797795384	21.74666054273357
18	20.480728748038427	20.71707429096337	32.78409308378306	26.018103877215143
19	20.06162207677881	27.87920541968079	34.532284609790636	17.52688789374976
20	21.543805258927545	22.04711601025759	36.3761625904237	20.032916140391166
21	26.796991617866574	22.001186512037357	32.66926933823248	18.532552531863587
22	25.000956864546254	25.143529681938226	30.770850078462892	19.084663375052628
23	23.09296895931412	24.179010219313355	31.372717878057184	21.355302943315344
24	21.634707390821756	26.04585294905653	33.66345160179125	18.655988058330465
25	19.768821525624833	28.224633520878783	32.01190339495541	19.994641558540973
26	21.704558502698358	26.70513262142611	32.574539748153256	19.01576912772228
27	19.48271902629464	29.43506717189115	31.783212768400503	19.29900103341371
28	18.09717916331764	27.86676618057948	33.895969686531174	20.14008496957171
29	18.101963486048913	27.671565813143488	35.10066215026601	19.125808550541585
30	18.25123435526467	29.781452137635394	34.9906227274467	16.976690779653232
31	17.12500478432273	30.66176752018984	31.969801354920197	20.24342634056723
32	18.715313660198262	29.41401615187354	33.77731848279558	18.09335170513262
33	17.972786772304513	28.581543996631837	35.02124239292685	18.424426838136792
34	17.423546522754236	29.89914647682474	31.195697937000038	21.481609063420983
35	18.049335936004898	29.19010984804991	34.025146400275574	18.735407815669614
36	16.506870287442112	31.14115665786351	30.66176752018984	21.690205534504535
37	16.448501550120564	30.906724844031082	31.90282083668236	20.741952769165998
38	16.494431048340797	27.772993455046503	33.461553182531475	22.27102231408122
39	18.36605810081525	28.080146974394303	31.736326405634017	21.817468519156428
40	16.255214911777088	27.274467026447734	35.192521146706476	21.277796915068702
41	16.014085046120872	28.696367742182417	31.593753588242045	23.695793623454662
42	16.177708883530446	27.248631683698854	34.35526466873349	22.218394764037203
43	15.08305584261492	28.606422474834464	33.63761625904237	22.67290542350825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	9.0
2	16.0
3	97.5
4	179.0
5	179.0
6	292.5
7	406.0
8	448.0
9	490.0
10	692.0
11	894.0
12	894.0
13	1694.5
14	2495.0
15	4136.5
16	5778.0
17	5302.0
18	4826.0
19	4826.0
20	5308.0
21	5790.0
22	4281.0
23	2772.0
24	2614.0
25	2456.0
26	2456.0
27	2494.0
28	2532.0
29	2456.5
30	2381.0
31	2188.0
32	1995.0
33	1995.0
34	1893.5
35	1792.0
36	1825.5
37	1859.0
38	2071.0
39	2283.0
40	2283.0
41	2686.5
42	3090.0
43	3564.5
44	4039.0
45	5074.5
46	6110.0
47	6110.0
48	7275.5
49	8441.0
50	9068.0
51	9695.0
52	7382.0
53	5069.0
54	5069.0
55	5636.5
56	6204.0
57	5661.0
58	5118.0
59	4379.5
60	3641.0
61	3641.0
62	3390.5
63	3140.0
64	2947.0
65	2754.0
66	2627.5
67	2501.0
68	2501.0
69	2215.5
70	1930.0
71	1676.5
72	1423.0
73	1245.0
74	1067.0
75	1067.0
76	842.5
77	618.0
78	501.5
79	385.0
80	297.0
81	209.0
82	209.0
83	142.5
84	76.0
85	55.0
86	34.0
87	23.5
88	13.0
89	13.0
90	9.0
91	5.0
92	2.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	104508.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.137635396333295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.73953898106005	43.65981551651548
2	9.154309205696668	9.545680713438205
3	2.6629716634855383	4.165231369847285
4	1.2534870063133168	2.6141539403682015
5	0.7212597269123476	1.8802388333907454
6	0.4331228894435472	1.3549201974968423
7	0.2973131698722654	1.0850843954529796
8	0.24592570841286154	1.0257587935851802
9	0.2092203787990016	0.9817430244574578
>10	1.101159888415798	10.594404256133503
>50	0.08442225811187784	2.9509702606499024
>100	0.08075172515049185	7.730508669192789
>500	0.009176332403464982	3.79014046771539
>1k	0.007341065922771986	8.621349561756038
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2882	2.757683622306426	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2684	2.5682244421479696	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1858	1.7778543269414784	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	1586	1.5175871703601638	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	985	0.9425115780610097	RNA PCR Primer, Index 46 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	927	0.8870134343782294	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	748	0.7157346805986144	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	741	0.7090366287748306	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	560	0.535844145902706	No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	354	0.3387300493742106	No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA	335	0.32054962299536877	No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA	328	0.31385157117158496	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	324	0.3100241129865656	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	309	0.29567114479274315	No Hit
ACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	264	0.25261224021127565	No Hit
GGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA	264	0.25261224021127565	No Hit
TATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA	257	0.24591418838749188	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	249	0.23825927201745323	No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA	243	0.2325180847399242	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	235	0.22486316836988554	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	216	0.20668274199104375	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	212	0.20285528380602444	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	205	0.19615723198224058	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	203	0.1942435028897309	No Hit
TATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAAAA	198	0.18945918015845678	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	188	0.17989053469590843	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	188	0.17989053469590843	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG	187	0.1789336701496536	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	180	0.17223561832586978	RNA PCR Primer, Index 46 (95% over 22bp)
GTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAA	172	0.16458070195583113	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	165	0.15788265013204733	No Hit
GGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA	159	0.15214146285451832	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	151	0.14448654648447967	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	151	0.14448654648447967	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	149	0.14257281739196997	No Hit
ACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	145	0.13874535920695066	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC	142	0.13587476556818617	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	139	0.13300417192942168	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	138	0.13204730738316683	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	134	0.12821984919814752	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127	0.1215217973743637	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	125	0.11960806828185402	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	123	0.11769433918934437	No Hit
GTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAAA	122	0.11673747464308953	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	122	0.11673747464308953	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	121	0.11578061009683469	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	120	0.11482374555057985	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	117	0.11195315191181536	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	108	0.10334137099552188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0028705936387644964	0.0
3	0.0	0.0	0.0	0.005741187277528993	0.0
4	0.0	0.0	0.0	0.005741187277528993	0.0
5	0.0	0.0	0.0	0.006698051823783825	0.0
6	0.0	0.0	0.0	0.006698051823783825	0.0
7	0.0	0.0	0.0	0.007654916370038657	0.0
8	0.0	0.0	0.0	0.007654916370038657	0.0
9	0.0	0.0	0.0	0.011482374555057986	0.0
10	0.0	0.0	0.0	0.01818042637884181	0.0
11	0.0	0.0	0.0	0.02296474911011597	0.0
12	0.0	0.0	0.0	0.028705936387644966	0.0
13	0.0	0.0	0.0	0.03061966548015463	0.0
14	0.0	0.0	0.0	0.033490259118919126	0.0
15	0.0	0.0	0.0	0.04114517548895778	0.0
16	0.0	0.0	0.0	0.0775060282466414	0.0
17	0.0	0.0	0.0	0.10812569372679603	0.0
18	0.0	0.0	0.0	0.11673747464308952	0.0
19	0.0	0.0	0.0	0.1454434110307345	0.0
20	0.0	0.0	0.0	0.17223561832586978	0.0
21	0.0	0.0	0.0	0.2172082519998469	0.0
22	0.0	0.0	0.0	0.3090672484403108	0.0
23	0.0	0.0	0.0	0.4640793049335936	0.0
24	0.0	0.0	0.0	0.6946836605810082	0.0
25	0.0	0.0	0.0	0.7549661269950626	0.0
26	0.0	0.0	0.0	0.8401270716117426	0.0
27	0.0	0.0	0.0	0.9032801316645616	0.0
28	0.0	0.0	0.0	0.9721743789949094	0.0
29	0.0	0.0	0.0	1.065947104527883	0.0
30	0.0	0.0	0.0	1.2793278983427105	0.0
31	0.0	0.0	0.0	1.829525012439239	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTGGC	25	0.0054737893	29.6	26
ATGCCCC	25	0.0054737893	29.6	37
CGTCTTC	40	5.8893933E-5	27.75	37
CCGTCTT	145	0.0	26.793102	37
GCCGTCT	155	0.0	23.870966	36
TACGGGA	45	0.0038029272	20.555555	2
GGCCTGT	45	0.0038029272	20.555555	34
AGTCAGA	50	0.0069932113	18.5	15
GTCAGAG	50	0.0069932113	18.5	16
TCAGAGG	50	0.0069932113	18.5	17
GTTCCCA	60	9.160449E-4	18.5	6
TGGATGC	50	0.0069932113	18.5	2
TATACAC	250	0.0	18.5	3
TGCCGTC	200	0.0	18.5	35
ATGCCGT	210	0.0	17.61905	34
TATGCCG	225	0.0	16.444443	33
GGTATCA	1600	0.0	16.41875	1
TTATACA	295	0.0	16.305084	2
AGTCGGT	160	6.039045E-10	16.1875	11
GGAACAG	140	3.369496E-8	15.857143	22
>>END_MODULE