FastQCFastQC Report
Fri 10 Feb 2017
ERR1630460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139297
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.13711709512767684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1740.1249129557707632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCA303.583398E-430.8333325
TGCACGA303.583398E-430.83333216
CTGACAC250.00547997629.5999983
CTAGATA358.8301435E-426.4285723
TCTAGAT358.8301435E-426.4285722
TAGATAA358.8301435E-426.4285724
GTGGGTA451.315071E-424.6666689
GATCGCA400.001922721723.12500220
CCGATCG400.001922721723.12500218
TAGAACT400.001922721723.1250024
AGGAATT400.001922721723.12500228
AGTATGG400.001922721723.1250022
ACGATGA400.001922721723.12500219
GCCGATC400.001922721723.12500217
TAACCTC400.001922721723.1250028
CCCCCGT400.001922721723.12500230
AACCTCG400.001922721723.1250029
CGATGAT400.001922721723.12500220
CGATCGC400.001922721723.12500219
GTCGGGA400.001922721723.1250022
Produced by FastQC (version 0.11.5)