FastQCFastQC Report
Fri 10 Feb 2017
ERR1630459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215872
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG9970.4618477616365253No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG7020.3251927067892084No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT6450.29878817076786246No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4230.19594945152683071No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG3830.17741995256448267No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC3630.16815520308330864No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG3280.15194189149125406No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT3110.14406685443225617No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC2890.1338756300029647No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA2880.13341239252890602No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC2770.1283167803142603No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC2760.12785354284020162No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC2680.12414764304773199No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA2650.12275793062555589No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT2600.12044174325526238No Hit
GTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAA2570.11905203083308628No Hit
ACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTC2510.11627260598873407No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA2510.11627260598873407No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG2360.10932404387785355No Hit
TTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAG2270.10515490661132523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG250.00548657929.67
GACGTTC250.00548657929.66
AGACGTT250.00548657929.65
TGCTAGA509.046498E-625.9000022
AAGTACC400.001926137523.12537
GTGCTAG603.7082682E-521.5833341
GGTATCA705.0703147E-621.1428571
ACTGTTC450.00381589920.5555578
GAGCATC450.00381589920.5555576
CCGGTGC450.00381589920.55555713
TTCGAAT450.00381589920.55555710
CTAGATT555.124516E-420.1818184
ACCGGTG500.00701683518.512
ACCGCAG701.2133858E-418.529
TCCTACA500.00701683518.52
TCTGTTA500.00701683518.510
CTGTTCA500.00701683518.59
ATTGCCG500.00701683518.511
TTCTGTT500.00701683518.59
TCGAATG500.00701683518.511
Produced by FastQC (version 0.11.5)