FastQCFastQC Report
Fri 10 Feb 2017
ERR1630458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172391
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC55940.2575042890529375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55550.2557090321217497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47450.2184229266278492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41400.19057342807993588No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT24310.11190434871070631No Hit
GTATATGTCCTAGCAACAAACATCAGACACACAGACCATATAT23250.10702493243619589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.020.1108191
GCGTTGC2600.018.51
ACTACGC701.21983176E-418.58
GACAGGC13800.017.829717
GGACCGT852.7249236E-517.4117646
TGCATCA13850.017.3646214
CGTTGCA2750.016.8181822
CCAGGAC17500.016.283
CAGGACA17850.016.2717084
GCATCAG14900.016.1409415
TTATACC2600.015.6538464
CGGTCGA1302.5918598E-715.65384620
ATCAAGC15300.015.59803930
GGACAGG16850.015.5905046
TCGAAAT1455.3521944E-815.31034523
CGATTAG855.3668825E-415.23529316
GGTCTAG2800.015.1964281
ACAGGCT16950.014.8436588
CGCGAAC1252.9614985E-614.79999928
GTTCGCG1508.111056E-814.79999930
Produced by FastQC (version 0.11.5)