FastQCFastQC Report
Fri 10 Feb 2017
ERR1630457.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630457.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294560
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG8430.2861895708853884No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT6180.20980445410103207No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC5790.1965643671917436No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG5020.17042368278109724No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA4480.1520912547528517No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT4380.14869636067354697No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG4330.14699891363389464No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC4280.14530146659424226No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG4200.14258555133079848No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC4110.13953014665942423No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA3930.13341933731667574No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA3910.13274035850081478No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC3870.1313824008690929No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG3710.12595057034220533No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG3660.12425312330255298No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA3650.12391363389462248No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT3610.12255567626290059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3440.11678435632808257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.11542639869636068No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG3300.11203150461705595No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC3280.11135252580119501No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA3250.11033405757740358No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG3190.10829712112982075No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA3190.10829712112982075No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC3160.10727865290602932No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC3120.10592069527430743No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT3110.10558120586637698No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA3050.10354426941879415No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA2960.10048886474741989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGG200.001839714837.05
AACGGTT250.005489790329.620
ACTACAA250.005489790329.618
GACGGAC556.2352046E-726.909097
CAAGACG556.2352046E-726.909094
CGCAAGA556.2352046E-726.909092
ACGCTTC358.853677E-426.42857231
ACGGACC509.061148E-625.9000028
CGGACCA551.8964372E-523.5454549
AACGCTT400.001927797623.12530
AAGACGG652.6716116E-622.769235
GAGCGAA603.7141886E-521.58333216
CTTGTAC450.003819157820.5555553
GTAAACG450.003819157820.55555527
TAGTACT450.003819157820.5555554
GCATTTG951.6655758E-719.47368424
GGTATCA1950.018.9743581
CCTAGGA609.2146016E-418.52
CACGAAT701.215295E-418.514
GCGCAAG801.6100288E-518.51
Produced by FastQC (version 0.11.5)