##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630457.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294560
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.914974877783813	33.0	31.0	34.0	30.0	34.0
2	32.114533541553506	33.0	31.0	34.0	30.0	34.0
3	32.269058935361215	34.0	31.0	34.0	30.0	34.0
4	35.915361895708855	37.0	35.0	37.0	35.0	37.0
5	35.828350760456274	37.0	35.0	37.0	35.0	37.0
6	35.886759913090714	37.0	35.0	37.0	35.0	37.0
7	35.87194120043455	37.0	35.0	37.0	35.0	37.0
8	35.85825298750679	37.0	35.0	37.0	35.0	37.0
9	37.60815114068441	39.0	37.0	39.0	35.0	39.0
10	37.50894215100489	39.0	37.0	39.0	35.0	39.0
11	37.59204915806627	39.0	37.0	39.0	35.0	39.0
12	37.44263986963607	39.0	37.0	39.0	35.0	39.0
13	37.488270640956	39.0	37.0	39.0	35.0	39.0
14	38.73644758283542	40.0	38.0	41.0	35.0	41.0
15	38.730795084193375	40.0	38.0	41.0	35.0	41.0
16	38.73291349809886	40.0	38.0	41.0	35.0	41.0
17	38.67455526887561	40.0	38.0	41.0	35.0	41.0
18	38.64457835415535	40.0	38.0	41.0	35.0	41.0
19	38.63785646387833	40.0	38.0	41.0	34.0	41.0
20	38.60133419337317	40.0	38.0	41.0	34.0	41.0
21	38.5504752851711	40.0	38.0	41.0	34.0	41.0
22	38.5342850353069	40.0	38.0	41.0	34.0	41.0
23	38.493481803367736	40.0	38.0	41.0	34.0	41.0
24	38.47869364475828	40.0	38.0	41.0	34.0	41.0
25	38.44961298207496	40.0	38.0	41.0	34.0	41.0
26	38.327756653992395	40.0	38.0	41.0	34.0	41.0
27	38.23187805540467	40.0	38.0	41.0	34.0	41.0
28	38.15213878326996	40.0	38.0	41.0	33.0	41.0
29	38.08369092884302	40.0	38.0	41.0	33.0	41.0
30	38.03326656708311	40.0	37.0	41.0	33.0	41.0
31	37.90365290602933	40.0	37.0	41.0	33.0	41.0
32	37.842364883215645	40.0	37.0	41.0	33.0	41.0
33	37.78108365019011	40.0	37.0	41.0	33.0	41.0
34	37.66790467137425	40.0	37.0	41.0	33.0	41.0
35	37.59991512764802	40.0	37.0	41.0	32.0	41.0
36	37.49507061379685	40.0	37.0	41.0	32.0	41.0
37	37.4008690928843	39.0	36.0	41.0	32.0	41.0
38	37.32940996740901	39.0	36.0	41.0	32.0	41.0
39	37.21958853883759	39.0	36.0	41.0	31.0	41.0
40	37.11377648017382	39.0	36.0	41.0	31.0	41.0
41	36.990562194459535	39.0	36.0	41.0	31.0	41.0
42	36.93901751765345	39.0	35.0	41.0	31.0	41.0
43	35.79423886474742	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	2.0
17	6.0
18	7.0
19	21.0
20	28.0
21	62.0
22	121.0
23	221.0
24	351.0
25	647.0
26	914.0
27	1409.0
28	1938.0
29	2811.0
30	3844.0
31	5226.0
32	7144.0
33	9486.0
34	12711.0
35	17823.0
36	25826.0
37	40782.0
38	72562.0
39	90616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56850896252037	18.12703693644758	11.236759913090712	26.06769418794134
2	19.264326453014665	20.192490494296578	33.495722433460074	27.04746061922868
3	20.614815317762087	21.016770776751763	28.817558392178167	29.550855513307983
4	14.131925583921781	15.217952199891362	34.00835143943509	36.64177077675176
5	15.903041825095057	35.02614068441064	33.61827810972298	15.452539380771322
6	37.15168386746333	33.514394350896254	15.326249321021185	14.007672460619228
7	32.07801466594242	28.840304182509506	19.809885931558934	19.271795219989134
8	28.69771863117871	32.33602661596958	19.37567897881586	19.59057577403585
9	27.19174361759913	12.860537751222163	18.28965236284628	41.658066268332426
10	17.165942422596416	25.65215915263444	32.52919608908202	24.652702335687128
11	38.47840847365562	20.766567083107006	19.637764801738186	21.117259641499185
12	22.582495926127105	23.862710483432917	28.024171645844646	25.530621944595328
13	32.07869364475828	17.95152091254753	23.914312873438348	26.055472569255837
14	23.97847637153721	19.207631721890277	23.71978544269419	33.094106463878326
15	25.99063009234112	26.57896523628463	21.241173275393805	26.189231395980446
16	27.590983161325365	24.523696360673547	22.376425855513308	25.508894622487777
17	25.247487778381316	24.991852254209668	23.640684410646386	26.119975556762633
18	25.218291689299292	23.229902227050516	25.511271048343293	26.0405350353069
19	27.2192422596415	24.224266702878868	24.073193916349812	24.483297121129823
20	26.984655078761545	23.53578218359587	24.24735198261814	25.232210755024443
21	26.163090711569797	23.962180879956545	23.791757197175446	26.082971211298204
22	27.25760456273764	23.236692015209126	23.896998913633894	25.60870450841934
23	24.854359043997828	23.817218902770236	24.394690385659967	26.93373166757197
24	25.701045627376423	24.37601846822379	24.060972297664314	25.861963606735472
25	26.085687126561652	23.89360401955459	24.193033677349266	25.82767517653449
26	26.6376969038566	24.195070613796847	23.126357957631722	26.04087452471483
27	26.70865019011407	23.578897338403042	23.28184410646388	26.43060836501901
28	25.862303096143403	24.393332428028245	24.130228136882128	25.614136338946224
29	25.871808799565454	24.560361216730037	24.66763986963607	24.90019011406844
30	26.16886203150462	23.868481803367736	23.831137968495383	26.131518196632264
31	25.391091797935907	23.731667571971755	24.154671374253123	26.72256925583922
32	24.92836773492667	24.342069527430745	24.027362846279196	26.70219989136339
33	25.32557034220532	23.697718631178706	24.47548886474742	26.501222161868547
34	26.55010863661054	23.637629005975015	24.57461977186312	25.237642585551328
35	26.749049429657795	23.646116241173274	24.000543183052688	25.604291146116243
36	25.07808256382401	24.206952743074417	23.434953829440524	27.280010863661055
37	25.32828625746877	23.80058392178164	25.378870179250406	25.49225964149919
38	25.067558392178164	23.54155350353069	24.549837045084193	26.841051059206954
39	24.717205323193916	24.15229494839761	25.74789516567083	25.382604562737644
40	26.02661596958175	22.919269418794133	25.10659967409017	25.94751493753395
41	24.039584464964694	23.57142857142857	26.248302552960347	26.140684410646386
42	24.379752851711025	24.16791146116241	25.786936447582836	25.665399239543724
43	24.67646659424226	22.44160782183596	25.766227593699075	27.115697990222703
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	8.5
2	14.0
3	35.5
4	57.0
5	57.0
6	82.5
7	108.0
8	95.5
9	83.0
10	132.0
11	181.0
12	181.0
13	299.5
14	418.0
15	625.0
16	832.0
17	839.5
18	847.0
19	847.0
20	1066.0
21	1285.0
22	1357.0
23	1429.0
24	1772.0
25	2115.0
26	2115.0
27	2529.5
28	2944.0
29	3572.0
30	4200.0
31	5039.5
32	5879.0
33	5879.0
34	7523.0
35	9167.0
36	10330.5
37	11494.0
38	12178.0
39	12862.0
40	12862.0
41	13654.0
42	14446.0
43	15464.0
44	16482.0
45	17082.0
46	17682.0
47	17682.0
48	18345.5
49	19009.0
50	19771.5
51	20534.0
52	22368.0
53	24202.0
54	24202.0
55	23916.5
56	23631.0
57	21756.0
58	19881.0
59	20139.0
60	20397.0
61	20397.0
62	19286.0
63	18175.0
64	15038.5
65	11902.0
66	10782.5
67	9663.0
68	9663.0
69	8694.5
70	7726.0
71	7809.0
72	7892.0
73	6888.5
74	5885.0
75	5885.0
76	3583.5
77	1282.0
78	1008.0
79	734.0
80	635.0
81	536.0
82	536.0
83	399.0
84	262.0
85	209.5
86	157.0
87	121.0
88	85.0
89	85.0
90	77.0
91	69.0
92	37.5
93	6.0
94	4.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	294560.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.67694682154695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.6283386967283	58.61564242413443
2	5.2322796871819985	6.768157501666609
3	1.3577474391414215	2.634448767553238
4	0.6639334021663678	1.717647413798517
5	0.43126183419802233	1.3946349358294146
6	0.25295515094136345	0.9816220107402568
7	0.19462997320810405	0.8811650698941755
8	0.14205847115500997	0.7350326547554272
9	0.11171942658910151	0.6503104271193333
>10	0.8005070456630752	10.01707819330994
>50	0.09517654136162251	4.31955263274975
>100	0.08728898268524496	10.420185270043273
>500	0.0021033489803673486	0.8645226984056268
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	843	0.2861895708853884	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	618	0.20980445410103207	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	579	0.1965643671917436	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	502	0.17042368278109724	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	448	0.1520912547528517	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	438	0.14869636067354697	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	433	0.14699891363389464	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	428	0.14530146659424226	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	420	0.14258555133079848	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	411	0.13953014665942423	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	393	0.13341933731667574	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	391	0.13274035850081478	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	387	0.1313824008690929	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	371	0.12595057034220533	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	366	0.12425312330255298	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	365	0.12391363389462248	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	361	0.12255567626290059	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	344	0.11678435632808257	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	340	0.11542639869636068	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	330	0.11203150461705595	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	328	0.11135252580119501	No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA	325	0.11033405757740358	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	319	0.10829712112982075	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	319	0.10829712112982075	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	316	0.10727865290602932	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	312	0.10592069527430743	No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT	311	0.10558120586637698	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	305	0.10354426941879415	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	296	0.10048886474741989	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.3948940793047255E-4	0.0
11	0.0	0.0	0.0	3.3948940793047255E-4	0.0
12	0.0	0.0	0.0	3.3948940793047255E-4	0.0
13	0.0	0.0	0.0	3.3948940793047255E-4	0.0
14	0.0	0.0	0.0	3.3948940793047255E-4	0.0
15	0.0	0.0	0.0	3.3948940793047255E-4	0.0
16	0.0	0.0	0.0	6.789788158609451E-4	0.0
17	0.0	0.0	0.0	6.789788158609451E-4	0.0
18	0.0	0.0	0.0	0.0010184682237914177	0.0
19	0.0	0.0	0.0	0.0013579576317218902	0.0
20	0.0	0.0	0.0	0.001697447039652363	0.0
21	0.0	0.0	0.0	0.0027159152634437804	0.0
22	0.0	0.0	0.0	0.004073872895165671	0.0
23	0.0	0.0	0.0	0.005092341118957088	0.0
24	0.0	0.0	0.0	0.006450298750678979	0.0
25	0.0	0.0	0.0	0.007129277566539924	0.0
26	0.0	0.0	0.0	0.011203150461705594	0.0
27	0.0	0.0	0.0	0.01833242802824552	0.0
28	0.0	0.0	0.0	0.04277566539923954	0.0
29	0.0	0.0	0.0	0.07502715915263444	0.0
30	0.0	0.0	0.0	0.1188212927756654	0.0
31	0.0	0.0	0.0	0.23662411732753938	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACGG	20	0.0018397148	37.0	5
AACGGTT	25	0.0054897903	29.6	20
ACTACAA	25	0.0054897903	29.6	18
GACGGAC	55	6.2352046E-7	26.90909	7
CAAGACG	55	6.2352046E-7	26.90909	4
CGCAAGA	55	6.2352046E-7	26.90909	2
ACGCTTC	35	8.853677E-4	26.428572	31
ACGGACC	50	9.061148E-6	25.900002	8
CGGACCA	55	1.8964372E-5	23.545454	9
AACGCTT	40	0.0019277976	23.125	30
AAGACGG	65	2.6716116E-6	22.76923	5
GAGCGAA	60	3.7141886E-5	21.583332	16
CTTGTAC	45	0.0038191578	20.555555	3
GTAAACG	45	0.0038191578	20.555555	27
TAGTACT	45	0.0038191578	20.555555	4
GCATTTG	95	1.6655758E-7	19.473684	24
GGTATCA	195	0.0	18.974358	1
CCTAGGA	60	9.2146016E-4	18.5	2
CACGAAT	70	1.215295E-4	18.5	14
GCGCAAG	80	1.6100288E-5	18.5	1
>>END_MODULE