FastQCFastQC Report
Fri 10 Feb 2017
ERR1630456.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630456.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433484
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT182034.199232266934881No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT146183.3722121231694824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT99932.305275396554429No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65661.5147041182604202No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25390.5857194267839182No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.47568076330383585No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA20460.47198973895230273No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16490.3804061972298862No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.30450950900148566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT12010.2770575153869578No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA11030.2544499912338172No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.25283516808002143No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA10810.24937483275045907No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9700.22376835131169776No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.2103883880373901No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8510.1963163576971699No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.18408983953271632No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA7490.17278607745614602No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA7250.16724954092884628No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7060.16286644951140067No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG6990.1612516263576049No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6250.14418063873176404No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA6170.14233512655599745No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC6060.1397975473143184No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA6050.13956685829234758No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA6000.13841341318249345No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA5630.12987791936957305No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5610.1294165413256314No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA5600.12918585230366056No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5290.12203449262256508No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA4920.11349899880964466No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA4760.1098079744581115No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4730.10911590739219902No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA4720.10888521837022822No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA4400.10150316966716189No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT4390.10127248064519105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAC200.001840671437.03
CGAATGA251.2323575E-436.99999617
CACACAT352.3849014E-531.7142877
GGCTTCT303.5958135E-430.8333326
TGTTCCA303.5958135E-430.83333210
ATGACAG303.5958135E-430.83333220
TATCGGC303.5958135E-430.8333322
AGTCACG250.00549261429.59999822
CGCGACG250.00549261429.59999830
GGTCTAA250.00549261429.59999830
TATTGAC803.274181E-1127.75000230
TAGCACA405.931725E-527.7500024
ACATCTG405.931725E-527.75000210
GCTTCTA358.860446E-426.4285727
TAACCCG358.860446E-426.42857229
TGACAGT358.860446E-426.42857221
GATATCT358.860446E-426.42857234
CTTCTAT358.860446E-426.4285728
CGGCTTC358.860446E-426.4285725
CTGTGCG358.860446E-426.4285729
Produced by FastQC (version 0.11.5)