FastQCFastQC Report
Fri 10 Feb 2017
ERR1630455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675701
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG16890.24996263140057512No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG12820.18972888896124174No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT10680.15805807598331215No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7620.1127717733139362No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA7580.11217979550126461No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG7060.10448408393653406No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC6760.1000442503414972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC200.001841398937.01
TACGCAT303.5979232E-430.83333228
CTATACA509.0838475E-625.9000024
CCGATCG400.001930367623.1250029
GTCTAGC502.7005048E-422.21
TCGAAGT705.09679E-621.14285727
ACGGACC450.003824199820.5555578
TTAGTCC450.003824199820.5555574
CTCTATA450.003824199820.5555572
GGTATCA4000.019.4250011
CGTGCAC801.6150634E-518.53
CGACCAT500.007031953418.510
TCTAGCA902.1495453E-618.52
ACGGAAT701.21825404E-418.518
TTAACCT500.007031953418.54
CTAGCAC902.1495453E-618.53
ATCTAGC953.6019374E-617.5263161
CTAGACA752.0656784E-417.2666664
AATCGAG752.0656784E-417.26666622
TCTATAC752.0656784E-417.2666663
Produced by FastQC (version 0.11.5)