##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630455.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	675701
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218602606774297	31.0	31.0	34.0	28.0	34.0
2	31.444600496373397	31.0	31.0	34.0	28.0	34.0
3	31.57245438440967	31.0	31.0	34.0	28.0	34.0
4	35.34064919246827	37.0	35.0	37.0	33.0	37.0
5	35.155408975271605	37.0	35.0	37.0	32.0	37.0
6	35.22620804172259	37.0	35.0	37.0	32.0	37.0
7	35.213499758029066	37.0	35.0	37.0	32.0	37.0
8	35.17237357943824	37.0	35.0	37.0	32.0	37.0
9	36.81751987935492	39.0	37.0	39.0	33.0	39.0
10	36.64146271797733	38.0	35.0	39.0	32.0	39.0
11	36.753386483074614	38.0	37.0	39.0	32.0	39.0
12	36.605041283052714	38.0	35.0	39.0	32.0	39.0
13	36.64862120967706	38.0	35.0	39.0	32.0	39.0
14	37.74207526701899	39.0	37.0	40.0	33.0	41.0
15	37.71297659763712	39.0	37.0	40.0	33.0	41.0
16	37.66923831694788	39.0	37.0	40.0	33.0	41.0
17	37.60313363455138	39.0	37.0	40.0	32.0	41.0
18	37.54516716713458	39.0	37.0	40.0	32.0	41.0
19	37.56301381824209	39.0	37.0	40.0	32.0	41.0
20	37.49904321586027	39.0	37.0	40.0	32.0	41.0
21	37.44065348430741	39.0	36.0	40.0	32.0	41.0
22	37.42138904633854	39.0	36.0	40.0	32.0	41.0
23	37.36231854030111	39.0	36.0	40.0	32.0	41.0
24	37.33064180754506	39.0	36.0	40.0	32.0	41.0
25	37.28934247544402	39.0	36.0	40.0	32.0	41.0
26	37.08570506777406	39.0	36.0	40.0	31.0	41.0
27	36.96984613016704	39.0	36.0	40.0	31.0	41.0
28	36.886765004047646	39.0	36.0	40.0	31.0	41.0
29	36.8096376947792	39.0	36.0	40.0	30.0	41.0
30	36.765418432117166	39.0	36.0	40.0	30.0	41.0
31	36.63639834778993	39.0	36.0	40.0	30.0	41.0
32	36.523987680941715	38.0	35.0	40.0	30.0	41.0
33	36.47177967769768	38.0	35.0	40.0	30.0	41.0
34	36.35267226184363	38.0	35.0	40.0	30.0	41.0
35	36.27278633596813	38.0	35.0	40.0	30.0	41.0
36	36.171713524177115	38.0	35.0	40.0	30.0	41.0
37	36.105376490489135	38.0	35.0	40.0	29.0	41.0
38	35.99133936460061	38.0	35.0	40.0	29.0	41.0
39	35.88992764551185	38.0	35.0	40.0	28.0	41.0
40	35.80790319978807	38.0	34.0	40.0	28.0	41.0
41	35.67467563315727	38.0	34.0	40.0	27.0	41.0
42	35.63407927470878	38.0	34.0	40.0	27.0	41.0
43	34.35259234483892	37.0	33.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	1.0
15	1.0
16	9.0
17	27.0
18	58.0
19	125.0
20	241.0
21	454.0
22	774.0
23	1218.0
24	2056.0
25	3107.0
26	4642.0
27	6396.0
28	8958.0
29	11846.0
30	15638.0
31	20255.0
32	25815.0
33	32725.0
34	41945.0
35	56108.0
36	77214.0
37	114046.0
38	169748.0
39	82290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.789785718831254	18.813054886702847	12.681496697503777	26.715662696962116
2	19.63975190209871	21.30202560008051	33.04375751996815	26.01446497785263
3	20.615923315194145	22.466741946511846	29.67362783242884	27.243706905865167
4	16.40178126123833	15.598171380536657	32.78373126575216	35.21631609247285
5	15.220637530505357	36.31458292943181	33.77588607978973	14.688893460273109
6	36.3893201282816	33.94548772312014	14.84857947524127	14.816612673357001
7	30.196048252111513	29.913526841013997	20.514991098133642	19.37543380874085
8	27.491153631561886	33.885697964040304	19.52653614542527	19.096612258972534
9	27.19723664757045	13.937821610446042	18.252747887009193	40.612193854974315
10	17.175496262400085	26.97568895117811	32.63751274602228	23.211302040399527
11	37.008676914789234	20.96075039107534	20.413170914354133	21.617401779781297
12	21.792479217878913	24.92374585800524	28.179327838792602	25.10444708532324
13	30.933208623340796	19.027054865983622	24.398217554806045	25.641518955869536
14	23.59431168519804	20.122953791691884	23.34716094840765	32.93557357470242
15	26.1713390982106	27.45223109037873	20.984725492488543	25.391704318922127
16	26.17903480977533	25.89902930438167	22.506848443320344	25.415087442522655
17	25.00899066302995	25.940911734628187	23.839242505190906	25.210855097150958
18	24.31430469985985	24.831545313681644	25.371281084384954	25.48286890207355
19	25.928184211655747	24.912202290658147	24.844124842200916	24.315488655485193
20	25.951419340803106	24.25451494078002	24.95319675418565	24.840868964231223
21	25.350857849847785	25.12783020892377	24.0033683537541	25.51794358747434
22	25.63633915000866	24.441875918490574	24.191617298183665	25.7301676333171
23	24.946832992699434	25.128126197830102	24.49071408803598	25.43432672143448
24	25.979390292451836	25.233942231845152	24.155062668251194	24.63160480745182
25	26.115841178272635	24.493821971552507	24.463039125293584	24.927297724881274
26	26.115989172725808	25.03222579217731	23.92537527693462	24.92640975816226
27	25.886301781409234	24.398069560352877	24.08950112549782	25.62612753274007
28	25.19812757417852	24.628200935028953	24.71448170122584	25.45918978956669
29	24.88304738338407	25.165568794481587	24.570779087199814	25.380604734934536
30	24.87816356642953	25.92226443352903	24.365510780655942	24.834061219385497
31	25.761246468482362	24.893406995105824	24.375722397924527	24.96962413848729
32	24.8386490474337	24.91264627401765	24.753256247955825	25.49544843059282
33	25.11599065267034	24.52667674015578	24.69879428919004	25.658538317983844
34	25.353225761098475	24.536000390705357	24.800910461875887	25.30986338632028
35	25.661498207047202	24.48479430990926	24.843236875481907	25.01047060756163
36	25.15136132697747	25.216034903011835	24.48227840420541	25.150325365805287
37	26.219881278849666	23.78048870728325	24.675263171136347	25.324366842730733
38	25.34686199961225	24.42382059520409	25.327622720700425	24.901694684483225
39	25.39052036329678	24.181553675368246	25.288108201704603	25.13981775963037
40	24.672451276526157	23.73623836578605	26.196350160795973	25.39496019689182
41	24.40502529965177	24.143223111997763	26.267239503863394	25.18451208448707
42	25.099267279462367	23.467480438833153	25.857738851947833	25.57551342975665
43	24.586762488141943	23.337985292311245	26.271827331911602	25.803424887635213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	13.5
2	14.0
3	26.0
4	38.0
5	38.0
6	74.0
7	110.0
8	105.0
9	100.0
10	164.5
11	229.0
12	229.0
13	399.0
14	569.0
15	953.0
16	1337.0
17	1394.5
18	1452.0
19	1452.0
20	2012.0
21	2572.0
22	3098.5
23	3625.0
24	4445.0
25	5265.0
26	5265.0
27	6150.5
28	7036.0
29	9702.0
30	12368.0
31	13912.0
32	15456.0
33	15456.0
34	17634.0
35	19812.0
36	22144.5
37	24477.0
38	28894.5
39	33312.0
40	33312.0
41	36396.0
42	39480.0
43	38638.5
44	37797.0
45	41638.5
46	45480.0
47	45480.0
48	49195.0
49	52910.0
50	55449.0
51	57988.0
52	61733.5
53	65479.0
54	65479.0
55	57992.0
56	50505.0
57	47775.0
58	45045.0
59	40925.5
60	36806.0
61	36806.0
62	34346.5
63	31887.0
64	28704.5
65	25522.0
66	22565.5
67	19609.0
68	19609.0
69	16879.5
70	14150.0
71	12292.5
72	10435.0
73	8518.0
74	6601.0
75	6601.0
76	5085.5
77	3570.0
78	3018.5
79	2467.0
80	1819.0
81	1171.0
82	1171.0
83	848.5
84	526.0
85	389.0
86	252.0
87	188.5
88	125.0
89	125.0
90	104.5
91	84.0
92	50.5
93	17.0
94	12.5
95	8.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	675701.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.45525964801488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.14310529012997	51.811159515589765
2	7.196923258339876	8.55789881982869
3	2.0269254228258133	3.615341319038132
4	0.9493806420158513	2.25782690303406
5	0.5564862037580743	1.6543015867487212
6	0.3512952944878448	1.25318117681404
7	0.23873726117172542	0.9935930095434612
8	0.18303237746506593	0.870579002094317
9	0.1376184413856383	0.7363926148444419
>10	0.9991314266163904	11.640868173145162
>50	0.12139507412678506	5.027699719935456
>100	0.09272878559959552	10.056724840631636
>500	0.0024927092903043466	0.9258346239786527
>1k	7.47812787091304E-4	0.5985986947734558
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1689	0.24996263140057512	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1282	0.18972888896124174	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1068	0.15805807598331215	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	762	0.1127717733139362	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	758	0.11217979550126461	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	706	0.10448408393653406	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	676	0.1000442503414972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.4799445316789528E-4	0.0
8	0.0	0.0	0.0	1.4799445316789528E-4	0.0
9	0.0	0.0	0.0	1.4799445316789528E-4	0.0
10	0.0	0.0	0.0	1.4799445316789528E-4	0.0
11	0.0	0.0	0.0	2.9598890633579056E-4	0.0
12	0.0	0.0	0.0	2.9598890633579056E-4	0.0
13	0.0	0.0	0.0	2.9598890633579056E-4	0.0
14	0.0	0.0	0.0	4.439833595036858E-4	0.0
15	0.0	0.0	0.0	4.439833595036858E-4	0.0
16	0.0	0.0	0.0	5.919778126715811E-4	0.0
17	0.0	0.0	0.0	0.0010359611721752668	0.0
18	0.0	0.0	0.0	0.0010359611721752668	0.0
19	0.0	0.0	0.0	0.0010359611721752668	0.0
20	0.0	0.0	0.0	0.0011839556253431622	0.0
21	0.0	0.0	0.0	0.0017759334380147431	0.0
22	0.0	0.0	0.0	0.0025159057038542194	0.0
23	0.0	0.0	0.0	0.004587828048204753	0.0
24	0.0	0.0	0.0	0.007399722658394763	0.0
25	0.0	0.0	0.0	0.007695711564730554	0.0
26	0.0	0.0	0.0	0.010063622815416878	0.0
27	0.0	0.0	0.0	0.01465145086362163	0.0
28	0.0	0.0	0.0	0.02871092391457168	0.0
29	0.0	0.0	0.0	0.04543429712254385	0.0
30	0.0	0.0	0.0	0.07236928759910079	0.0
31	0.0	0.0	0.0	0.13363899121060943	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	20	0.0018413989	37.0	1
TACGCAT	30	3.5979232E-4	30.833332	28
CTATACA	50	9.0838475E-6	25.900002	4
CCGATCG	40	0.0019303676	23.125002	9
GTCTAGC	50	2.7005048E-4	22.2	1
TCGAAGT	70	5.09679E-6	21.142857	27
ACGGACC	45	0.0038241998	20.555557	8
TTAGTCC	45	0.0038241998	20.555557	4
CTCTATA	45	0.0038241998	20.555557	2
GGTATCA	400	0.0	19.425001	1
CGTGCAC	80	1.6150634E-5	18.5	3
CGACCAT	50	0.0070319534	18.5	10
TCTAGCA	90	2.1495453E-6	18.5	2
ACGGAAT	70	1.21825404E-4	18.5	18
TTAACCT	50	0.0070319534	18.5	4
CTAGCAC	90	2.1495453E-6	18.5	3
ATCTAGC	95	3.6019374E-6	17.526316	1
CTAGACA	75	2.0656784E-4	17.266666	4
AATCGAG	75	2.0656784E-4	17.266666	22
TCTATAC	75	2.0656784E-4	17.266666	3
>>END_MODULE