FastQCFastQC Report
Fri 10 Feb 2017
ERR1630454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196607
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6010.30568596235128964No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG4150.21108098897801197No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT3810.1937876067484881No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA2630.1337693978342582No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2180.11088109782459424No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC2160.10986384004638697No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG2140.10884658226817967No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC2080.10579480893355782No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT2030.10325166448803959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG251.2299066E-437.01
TCTAGGC250.005485404729.63
TCGACAA250.005485404729.631
AGGCCCG405.9154965E-527.7510
TAAATCA358.8431593E-426.428574
TCTTATA1300.024.19230837
CCAGAAC400.00192552923.1253
TGCGTAC400.00192552923.12516
TACAAAG400.00192552923.1257
GTACCGG450.003814707320.5555536
GGATATA450.003814707320.5555532
TGTACCG450.003814707320.5555535
GTGCGTA450.003814707320.55555315
GCGTACT450.003814707320.55555317
TGTCCGA450.003814707320.55555312
GTTCCTC656.8650086E-519.92307711
ATGAATA759.201873E-619.73333235
GAGGTCG851.234599E-619.5882349
TTTGATG851.234599E-619.58823433
TCGCCTT801.6055916E-518.514