##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630454.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	196607
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80835372087464	31.0	31.0	34.0	30.0	34.0
2	32.00901799020381	33.0	31.0	34.0	30.0	34.0
3	32.15164261699736	34.0	31.0	34.0	30.0	34.0
4	35.81657316372255	37.0	35.0	37.0	35.0	37.0
5	35.71847899617003	37.0	35.0	37.0	35.0	37.0
6	35.788761336066365	37.0	35.0	37.0	35.0	37.0
7	35.76708865910166	37.0	35.0	37.0	35.0	37.0
8	35.731393083664365	37.0	35.0	37.0	33.0	37.0
9	37.46852350119782	39.0	37.0	39.0	35.0	39.0
10	37.36183350541944	39.0	37.0	39.0	34.0	39.0
11	37.44032002929703	39.0	37.0	39.0	35.0	39.0
12	37.31880350140127	39.0	37.0	39.0	34.0	39.0
13	37.35210343477089	39.0	37.0	39.0	34.0	39.0
14	38.56371848408246	40.0	38.0	41.0	34.0	41.0
15	38.5611397356147	40.0	38.0	41.0	34.0	41.0
16	38.53477241400357	40.0	38.0	41.0	34.0	41.0
17	38.48052714298067	40.0	38.0	41.0	34.0	41.0
18	38.448422487500444	40.0	38.0	41.0	34.0	41.0
19	38.46008534792759	40.0	38.0	41.0	34.0	41.0
20	38.40220338034759	40.0	38.0	41.0	34.0	41.0
21	38.35275956603783	40.0	38.0	41.0	34.0	41.0
22	38.33365037867421	40.0	38.0	41.0	34.0	41.0
23	38.28083944111858	40.0	38.0	41.0	34.0	41.0
24	38.26581454373446	40.0	38.0	41.0	34.0	41.0
25	38.22178762709365	40.0	38.0	41.0	34.0	41.0
26	38.05652901473498	40.0	38.0	41.0	33.0	41.0
27	37.95395382666945	40.0	37.0	41.0	33.0	41.0
28	37.862187002497365	40.0	37.0	41.0	33.0	41.0
29	37.799106847670735	40.0	37.0	41.0	33.0	41.0
30	37.74560417482592	40.0	37.0	41.0	32.0	41.0
31	37.6480745853403	40.0	37.0	41.0	32.0	41.0
32	37.55649086756829	40.0	37.0	41.0	32.0	41.0
33	37.50181326198965	40.0	37.0	41.0	32.0	41.0
34	37.405021184393235	40.0	37.0	41.0	31.0	41.0
35	37.31620440777795	39.0	37.0	41.0	31.0	41.0
36	37.236181824655276	39.0	36.0	41.0	31.0	41.0
37	37.18164154887669	39.0	36.0	41.0	31.0	41.0
38	37.0651451881164	39.0	36.0	41.0	31.0	41.0
39	36.974024322633475	39.0	36.0	41.0	31.0	41.0
40	36.88942407950887	39.0	36.0	41.0	30.0	41.0
41	36.78777459602151	39.0	35.0	40.0	30.0	41.0
42	36.74707411231543	39.0	35.0	40.0	30.0	41.0
43	35.44945500414533	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	12.0
18	9.0
19	11.0
20	50.0
21	47.0
22	108.0
23	235.0
24	344.0
25	500.0
26	804.0
27	1121.0
28	1596.0
29	2295.0
30	3033.0
31	4112.0
32	5395.0
33	6874.0
34	9337.0
35	12743.0
36	17493.0
37	25944.0
38	47694.0
39	56850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74364595360287	17.728259929707487	12.335776447430662	27.19231766925898
2	19.46777073044195	20.11322079071447	33.17735380734155	27.24165467150203
3	20.366517977488087	21.32019714455742	29.631701821400053	28.681583056554445
4	15.634234793267787	14.808221477363471	32.99221289170782	36.56533083766092
5	15.385006637607002	36.11519427080419	33.757699369808805	14.742099721779997
6	37.28300620018616	33.662585767546425	14.54983800169882	14.504570030568598
7	31.142329622037874	29.39976704796879	19.816181519477944	19.641721810515396
8	28.10988418520195	33.26127757404365	19.386390108185363	19.242448132569034
9	27.118057851449844	13.84335247473386	17.86152069865264	41.17706897516365
10	17.486661207383257	26.86781243801085	31.636208273357507	24.00931808124838
11	38.29263454505689	20.24699018854873	19.829405870594638	21.63096939579974
12	22.668572329571173	24.397910552523562	27.40187277156968	25.531644346335586
13	31.881367397905464	17.62653415188676	24.081034754611995	26.411063695595782
14	23.918273510098825	19.220577090337578	22.646701287339717	34.21444811222388
15	27.061091415870237	26.693861357937408	20.366009348598983	25.87903787759337
16	27.19231766925898	24.8917892038432	21.60299480689904	26.31289831999878
17	26.04383363766295	25.199509681750904	22.845575182979243	25.9110814976069
18	25.16950057729379	23.59478553662891	24.78548576602054	26.450228120056764
19	26.75133642240612	24.06119822793695	23.80535789671782	25.382107452939113
20	26.805251084651104	23.008845056381514	24.345013148056783	25.840890710910596
21	26.739637957956734	23.923868427878965	23.218908787581316	26.11758482658298
22	26.87137284023458	23.20721032313193	23.36081624764123	26.560600588992255
23	25.9944966354199	23.67870930333101	23.639036249980926	26.687757811268163
24	26.435986511161865	24.256003092463647	23.51543942992874	25.792570966445755
25	27.018875218074633	23.610553032191124	23.373531969868825	25.997039779865418
26	27.126704542564607	23.94573947011042	23.02969884083476	25.897857146490207
27	26.958348380271303	23.06123383195919	23.49204250102997	26.488375286739537
28	25.98178091319231	23.49000798547356	24.138509819080703	26.38970128225343
29	25.4227977640674	24.25244269023992	24.069336290162607	26.25542325553007
30	25.44314291963155	25.266140066223482	23.524086121043503	25.766630893101468
31	26.740146586845842	23.902506014536616	23.295711749835966	26.06163564878158
32	25.584033121913258	23.79772846338126	23.869445136744876	26.7487932779606
33	25.536222006337518	23.37861825875986	23.952860274557874	27.13229946034475
34	26.172516746606174	23.6075012588565	24.215821410224457	26.004160584312867
35	26.332734846673823	23.46915420102031	24.382651685850455	25.815459266455417
36	25.398892206279534	24.367392819177343	24.27024470135855	25.963470273184576
37	26.8459413957794	22.929498949681342	24.193441739103896	26.03111791543536
38	25.673043177506393	23.245357489814705	25.45840178630466	25.62319754637424
39	25.94872003540057	22.765211818500866	25.121180832828944	26.16488731326962
40	24.84702986160208	22.628390647331987	26.223379635516537	26.301199855549395
41	24.469118596998072	22.787082860732326	26.656731449032844	26.08706709323676
42	25.206630486198357	22.38170563611672	26.448193604500347	25.963470273184576
43	24.19547625466031	21.960560915938903	26.973607246944415	26.870355582456373
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	2.5
2	4.0
3	7.0
4	10.0
5	10.0
6	13.0
7	16.0
8	14.0
9	12.0
10	14.5
11	17.0
12	17.0
13	36.0
14	55.0
15	102.0
16	149.0
17	168.0
18	187.0
19	187.0
20	251.0
21	315.0
22	380.0
23	445.0
24	600.0
25	755.0
26	755.0
27	927.5
28	1100.0
29	1728.5
30	2357.0
31	2721.0
32	3085.0
33	3085.0
34	3807.0
35	4529.0
36	5190.5
37	5852.0
38	7279.5
39	8707.0
40	8707.0
41	9561.5
42	10416.0
43	10517.5
44	10619.0
45	11998.0
46	13377.0
47	13377.0
48	15043.0
49	16709.0
50	17292.5
51	17876.0
52	19204.5
53	20533.0
54	20533.0
55	18275.0
56	16017.0
57	15133.0
58	14249.0
59	13220.5
60	12192.0
61	12192.0
62	11498.5
63	10805.0
64	9706.0
65	8607.0
66	7396.5
67	6186.0
68	6186.0
69	5225.5
70	4265.0
71	3667.5
72	3070.0
73	2430.0
74	1790.0
75	1790.0
76	1391.5
77	993.0
78	798.5
79	604.0
80	478.5
81	353.0
82	353.0
83	259.0
84	165.0
85	124.0
86	83.0
87	66.0
88	49.0
89	49.0
90	43.0
91	37.0
92	23.5
93	10.0
94	7.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	196607.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.96106257456337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.0487519012186	52.1953565978661
2	6.762176692936035	8.109345996507832
3	2.001078252458463	3.599603349367795
4	0.8973825231442547	2.152320384942889
5	0.5988360885221762	1.7953424087892487
6	0.40424410033919544	1.4543343477501942
7	0.2926753846092594	1.2284388935413142
8	0.23592151686152243	1.1316883868175724
9	0.18316062723384405	0.9884255247688319
>10	1.3776814568589966	16.347757716412424
>50	0.15031060215309677	6.238517372162845
>100	0.04678541921321555	4.400148303442833
>500	9.954344513450118E-4	0.3587207176301062
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	601	0.30568596235128964	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	415	0.21108098897801197	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	381	0.1937876067484881	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	263	0.1337693978342582	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	218	0.11088109782459424	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	216	0.10986384004638697	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	214	0.10884658226817967	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	208	0.10579480893355782	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	203	0.10325166448803959	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	5.086288891036433E-4	0.0
18	0.0	0.0	0.0	5.086288891036433E-4	0.0
19	0.0	0.0	0.0	5.086288891036433E-4	0.0
20	0.0	0.0	0.0	0.0010172577782072865	0.0
21	0.0	0.0	0.0	0.002034515556414573	0.0
22	0.0	0.0	0.0	0.0025431444455182167	0.0
23	0.0	0.0	0.0	0.00305177333462186	0.0
24	0.0	0.0	0.0	0.00457766000193279	0.0
25	0.0	0.0	0.0	0.005594917780140076	0.0
26	0.0	0.0	0.0	0.009663948892969223	0.0
27	0.0	0.0	0.0	0.01729338222952387	0.0
28	0.0	0.0	0.0	0.04119894001739511	0.0
29	0.0	0.0	0.0	0.08646691114761937	0.0
30	0.0	0.0	0.0	0.13427802672336184	0.0
31	0.0	0.0	0.0	0.2782200023396929	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	25	1.2299066E-4	37.0	1
TCTAGGC	25	0.0054854047	29.6	3
TCGACAA	25	0.0054854047	29.6	31
AGGCCCG	40	5.9154965E-5	27.75	10
TAAATCA	35	8.8431593E-4	26.42857	4
TCTTATA	130	0.0	24.192308	37
CCAGAAC	40	0.001925529	23.125	3
TGCGTAC	40	0.001925529	23.125	16
TACAAAG	40	0.001925529	23.125	7
GTACCGG	45	0.0038147073	20.555553	6
GGATATA	45	0.0038147073	20.555553	2
TGTACCG	45	0.0038147073	20.555553	5
GTGCGTA	45	0.0038147073	20.555553	15
GCGTACT	45	0.0038147073	20.555553	17
TGTCCGA	45	0.0038147073	20.555553	12
GTTCCTC	65	6.8650086E-5	19.923077	11
ATGAATA	75	9.201873E-6	19.733332	35
GAGGTCG	85	1.234599E-6	19.588234	9
TTTGATG	85	1.234599E-6	19.588234	33
TCGCCTT	80	1.6055916E-5	18.5	14
>>END_MODULE