FastQCFastQC Report
Fri 10 Feb 2017
ERR1630453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427750
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26220.6129748684979545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.5594389246054938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20330.47527761542957336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.24850964348334306No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6300.1472822910578609No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6080.14213909994155463No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5340.12483927527761542No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4830.11291642314436001No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC4630.10824079485680888No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.1000584453535944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.025.9552231
CATGCCG609.2238985E-418.59
CGGACAG500.00702784118.4999985
GGGCGCT500.00702784118.49999818
GACGGGC500.00702784118.49999815
CTATACA803.3763587E-416.18754
TATAGTG803.3763587E-416.18755
CTAATAC957.043124E-515.5789483
GTATCAA11250.015.4577772
TTGGACT750.004098805614.84
AGTAGAG750.004098805614.85
GTCGCCA1001.0908171E-414.79999912
TGTAGGA908.25905E-414.3888882
GTGGTAT1956.675691E-1014.2307681
CTTAAGC800.006290099613.87531
GGACTGC800.006290099613.8756
TATACAG1203.290847E-513.8749995
TGGTATC2151.9463187E-1013.7674422
AAAGTGA1356.543436E-613.7037036
TTGGCCG1254.8304995E-513.3231
Produced by FastQC (version 0.11.5)