##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630453.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427750
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.037949736995905	33.0	31.0	34.0	30.0	34.0
2	32.27956049094097	34.0	31.0	34.0	30.0	34.0
3	32.36504266510812	34.0	31.0	34.0	30.0	34.0
4	35.987336060783164	37.0	35.0	37.0	35.0	37.0
5	35.91299824663939	37.0	35.0	37.0	35.0	37.0
6	35.96190999415546	37.0	35.0	37.0	35.0	37.0
7	35.96772647574518	37.0	35.0	37.0	35.0	37.0
8	35.96956633547633	37.0	35.0	37.0	35.0	37.0
9	37.71854354178843	39.0	37.0	39.0	35.0	39.0
10	37.643869082407946	39.0	37.0	39.0	35.0	39.0
11	37.69098305084746	39.0	37.0	39.0	35.0	39.0
12	37.64941905318527	39.0	37.0	39.0	35.0	39.0
13	37.63472822910579	39.0	37.0	39.0	35.0	39.0
14	38.923625949736994	40.0	38.0	41.0	36.0	41.0
15	38.920355347749855	40.0	38.0	41.0	36.0	41.0
16	38.87489888953828	40.0	38.0	41.0	35.0	41.0
17	38.88606428988896	40.0	38.0	41.0	36.0	41.0
18	38.85871887784921	40.0	38.0	41.0	35.0	41.0
19	38.9114178842782	40.0	38.0	41.0	35.0	41.0
20	38.892474576271184	40.0	38.0	41.0	35.0	41.0
21	38.84867562828755	40.0	38.0	41.0	35.0	41.0
22	38.81734190531853	40.0	38.0	41.0	35.0	41.0
23	38.7742910578609	40.0	38.0	41.0	35.0	41.0
24	38.74393220338983	40.0	38.0	41.0	35.0	41.0
25	38.73403623611923	40.0	38.0	41.0	35.0	41.0
26	38.60340853302162	40.0	38.0	41.0	34.0	41.0
27	38.49657042665108	40.0	38.0	41.0	34.0	41.0
28	38.45914903565166	40.0	38.0	41.0	34.0	41.0
29	38.40899590882525	40.0	38.0	41.0	34.0	41.0
30	38.35877264757452	40.0	38.0	41.0	34.0	41.0
31	38.29388427819988	40.0	38.0	41.0	34.0	41.0
32	38.20002571595558	40.0	38.0	41.0	34.0	41.0
33	38.125793103448274	40.0	38.0	41.0	34.0	41.0
34	38.06620455873758	40.0	38.0	41.0	33.0	41.0
35	37.97146697837522	40.0	37.0	41.0	33.0	41.0
36	37.90405844535359	40.0	37.0	41.0	33.0	41.0
37	37.8474926943308	40.0	37.0	41.0	33.0	41.0
38	37.751530099357105	40.0	37.0	41.0	33.0	41.0
39	37.68661835184103	40.0	37.0	41.0	33.0	41.0
40	37.58882057276446	40.0	37.0	41.0	33.0	41.0
41	37.49195791934541	40.0	37.0	41.0	33.0	41.0
42	37.44268147282291	40.0	37.0	41.0	32.0	41.0
43	36.41077732320281	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	3.0
11	2.0
12	0.0
13	0.0
14	1.0
15	0.0
16	2.0
17	4.0
18	9.0
19	13.0
20	38.0
21	67.0
22	149.0
23	239.0
24	385.0
25	697.0
26	1064.0
27	1689.0
28	2461.0
29	3510.0
30	5058.0
31	6853.0
32	8834.0
33	11833.0
34	16230.0
35	22576.0
36	33267.0
37	52559.0
38	98487.0
39	161717.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.37346580946815	23.348451198129748	17.709643483343072	21.56843950905903
2	16.723085914669785	22.496317942723554	38.182583284628876	22.59801285797779
3	17.2114552893045	25.71525423728814	33.586908240794855	23.486382232612506
4	14.25926358854471	19.197428404441848	36.47200467562829	30.071303331385156
5	13.185271770894214	37.9728813559322	35.03705435417884	13.804792518994741
6	28.829222676797194	37.73302162478083	18.133255406195207	15.30450029222677
7	24.239859731151373	33.66616014026885	23.931502045587376	18.162478082992404
8	23.941087083576857	35.97054354178843	21.96002337814144	18.128345996493277
9	24.940268848626534	16.4958503798948	22.400935125657508	36.16294564582116
10	16.896084161309176	28.69760374050263	34.03459964932788	20.371712448860315
11	31.296317942723554	24.21437755698422	24.069433080070134	20.41987142022209
12	19.945762711864408	28.262770309760377	30.177206312098185	21.61426066627703
13	27.20958503798948	22.70134424313267	27.750789012273525	22.338281706604324
14	21.476797194623025	23.946697837521917	27.588778492109878	26.987726475745177
15	23.60187025131502	29.139216832261834	25.498071303331386	21.76084161309176
16	22.239158386908244	28.71583869082408	26.935125657510227	22.10987726475745
17	21.02746931618936	29.014377556984222	28.009351256575105	21.948801870251312
18	20.442314436002338	27.3052016364699	30.01052016364699	22.241963763880772
19	22.008649912331972	27.723904149620104	30.272121566335475	19.99532437171245
20	22.54237288135593	26.56271186440678	30.418468731735825	20.476446522501462
21	21.935242548217417	27.536177673874928	29.384453535943894	21.144126241963765
22	21.62945645821157	27.32250146113384	29.83237872589129	21.215663354763294
23	21.42583284628872	27.35967270601987	29.848509643483347	21.365984804208065
24	22.32612507305669	27.498772647574516	29.514202220923437	20.660900058445353
25	22.25458796025716	26.744126241963762	29.97241379310345	21.028872004675627
26	21.718527177089424	27.32717708942139	29.38866160140269	21.5656341320865
27	21.682057276446525	27.210753945061366	29.225014611338402	21.88217416715371
28	21.318293395675045	27.556984219754533	29.639509059029805	21.48521332554062
29	21.554646405610754	27.94132086499123	29.641613091759204	20.86241963763881
30	21.13594389246055	27.950438340151955	29.597428404441846	21.316189362945646
31	21.621741671537112	27.51724137931034	30.072238457042666	20.788778492109877
32	21.080070134424314	27.295382817066045	29.949269433080072	21.67527761542957
33	21.29982466393922	27.448977206312097	29.87843366452367	21.372764465225014
34	21.51490356516657	27.483810637054358	29.50578609000584	21.495499707773234
35	21.643483343074227	27.48310929281122	29.312215078901225	21.561192285213327
36	21.445470485096436	27.48825248392753	29.452483927527762	21.613793103448277
37	22.093278784336647	26.334307422559906	29.561659848042083	22.010753945061367
38	21.426300409117474	27.36551724137931	29.764815897136177	21.443366452367037
39	21.091057860900058	27.295850379894798	30.151724137931033	21.461367621274107
40	21.411104617182932	26.64991233196961	30.484862653419054	21.454120397428404
41	20.852834599649327	27.461835184102863	30.2562244301578	21.429105786090005
42	21.17825832846289	26.967387492694332	29.919579193454123	21.93477498538866
43	20.703214494447693	27.315721800116894	30.064523670368203	21.91654003506721
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	382.0
1	328.5
2	275.0
3	391.0
4	507.0
5	507.0
6	672.5
7	838.0
8	755.0
9	672.0
10	986.0
11	1300.0
12	1300.0
13	2168.5
14	3037.0
15	4655.5
16	6274.0
17	6219.5
18	6165.0
19	6165.0
20	7373.0
21	8581.0
22	8831.5
23	9082.0
24	10044.0
25	11006.0
26	11006.0
27	11766.5
28	12527.0
29	15405.5
30	18284.0
31	18904.0
32	19524.0
33	19524.0
34	20720.0
35	21916.0
36	22992.0
37	24068.0
38	25548.5
39	27029.0
40	27029.0
41	28712.5
42	30396.0
43	28569.5
44	26743.0
45	27555.5
46	28368.0
47	28368.0
48	27828.0
49	27288.0
50	28030.0
51	28772.0
52	28490.5
53	28209.0
54	28209.0
55	24098.0
56	19987.0
57	18322.5
58	16658.0
59	14987.0
60	13316.0
61	13316.0
62	12152.5
63	10989.0
64	9629.0
65	8269.0
66	7137.5
67	6006.0
68	6006.0
69	5148.0
70	4290.0
71	3618.0
72	2946.0
73	2440.0
74	1934.0
75	1934.0
76	1449.0
77	964.0
78	752.0
79	540.0
80	414.5
81	289.0
82	289.0
83	214.0
84	139.0
85	110.5
86	82.0
87	71.0
88	60.0
89	60.0
90	45.5
91	31.0
92	18.5
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	427750.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.318134383565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.57987896872287	57.35348948988649
2	5.338833017812185	6.7608989294649176
3	1.4044884714301213	2.667887693225407
4	0.6266336732744319	1.5870910053462979
5	0.3601272324447633	1.1401292249559438
6	0.2509928091062209	0.9535437849777704
7	0.18851512679598648	0.8355498292262148
8	0.13639198384378046	0.6908868769489216
9	0.12211856919768053	0.6959087977668676
>10	0.7789727458751077	9.966918385605673
>50	0.12575714672124616	5.664998287375757
>100	0.08470113704916658	9.368125862129851
>500	0.0011096218827401736	0.4149976007523336
>1k	0.0014794958436535647	1.8995742323375722
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2622	0.6129748684979545	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2393	0.5594389246054938	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2033	0.47527761542957336	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1063	0.24850964348334306	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	630	0.1472822910578609	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	608	0.14213909994155463	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	534	0.12483927527761542	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	483	0.11291642314436001	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	463	0.10824079485680888	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	428	0.1000584453535944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.35125657510228E-4	0.0
2	0.0	0.0	0.0	9.35125657510228E-4	0.0
3	0.0	0.0	0.0	9.35125657510228E-4	0.0
4	0.0	0.0	0.0	9.35125657510228E-4	0.0
5	0.0	0.0	0.0	0.0011689070718877848	0.0
6	0.0	0.0	0.0	0.0011689070718877848	0.0
7	0.0	0.0	0.0	0.0011689070718877848	0.0
8	0.0	0.0	0.0	0.0011689070718877848	0.0
9	0.0	0.0	0.0	0.0016364699006428988	0.0
10	0.0	0.0	0.0	0.003039158386908241	0.0
11	0.0	0.0	0.0	0.003974284044418469	0.0
12	0.0	0.0	0.0	0.004441846873173583	0.0
13	0.0	0.0	0.0	0.004675628287551139	0.0
14	0.0	0.0	0.0	0.005143191116306254	0.0
15	0.0	0.0	0.0	0.006312098188194039	0.0
16	0.0	0.0	0.0	0.008416130917592052	0.0
17	0.0	0.0	0.0	0.01005260081823495	0.0
18	0.0	0.0	0.0	0.011689070718877849	0.0
19	0.0	0.0	0.0	0.013793103448275862	0.0
20	0.0	0.0	0.0	0.014260666277030976	0.0
21	0.0	0.0	0.0	0.01566335476329632	0.0
22	0.0	0.0	0.0	0.020572764465225015	0.0
23	0.0	0.0	0.0	0.028521332554061953	0.0
24	0.0	0.0	0.0	0.03810637054354179	0.0
25	0.0	0.0	0.0	0.043950905902980716	0.0
26	0.0	0.0	0.0	0.05143191116306254	0.0
27	0.0	0.0	0.0	0.06569257744009352	0.0
28	0.0	0.0	0.0	0.1005260081823495	0.0
29	0.0	0.0	0.0	0.15756867329047342	0.0
30	0.0	0.0	0.0	0.24079485680888368	0.0
31	0.0	0.0	0.0	0.38293395675043834	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	670	0.0	25.955223	1
CATGCCG	60	9.2238985E-4	18.5	9
CGGACAG	50	0.007027841	18.499998	5
GGGCGCT	50	0.007027841	18.499998	18
GACGGGC	50	0.007027841	18.499998	15
CTATACA	80	3.3763587E-4	16.1875	4
TATAGTG	80	3.3763587E-4	16.1875	5
CTAATAC	95	7.043124E-5	15.578948	3
GTATCAA	1125	0.0	15.457777	2
TTGGACT	75	0.0040988056	14.8	4
AGTAGAG	75	0.0040988056	14.8	5
GTCGCCA	100	1.0908171E-4	14.799999	12
TGTAGGA	90	8.25905E-4	14.388888	2
GTGGTAT	195	6.675691E-10	14.230768	1
CTTAAGC	80	0.0062900996	13.875	31
GGACTGC	80	0.0062900996	13.875	6
TATACAG	120	3.290847E-5	13.874999	5
TGGTATC	215	1.9463187E-10	13.767442	2
AAAGTGA	135	6.543436E-6	13.703703	6
TTGGCCG	125	4.8304995E-5	13.32	31
>>END_MODULE