FastQCFastQC Report
Fri 10 Feb 2017
ERR1630452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120736
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44290.20884259049688408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37380.1762595627178489No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32700.15419175229731563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.023.5958081
GTACGAA500.00703679718.4999981
CGAACGA1603.45608E-1117.3437516
CAACCGT650.001580529417.0769236
TCTAGCG1708.54925E-1116.3235328
CTAGTAC1251.6595368E-716.2799993
GTATCAA24300.016.1399172
TAGGACA7100.015.8943664
ACGAACG1701.4879333E-915.23529415
TAACGAG1601.097942E-815.0312513
CTAGCGG1752.240995E-914.829
ACCGACC1252.9614166E-614.7999998
CGAATTA908.27808E-414.38888815
GACGGAC2201.8189894E-1114.2954547
TTAGGAC7800.013.993593
ATCTCGC3750.013.81333311
ACGGACC2304.0017767E-1113.6739138
CGGTCCA2850.013.63157810
ATAACGA2202.8558134E-1013.45454512
GCTTAGG6650.013.3533831
Produced by FastQC (version 0.11.5)