FastQCFastQC Report
Fri 10 Feb 2017
ERR1630451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054965
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60660.2951875092763137No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG48210.23460253580961232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46450.22603791305448023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43710.2127043526288769No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT33680.16389573545048214No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC29460.14336010588988135No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG29400.14306813011413821No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA27280.13275165270454728No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA22780.11085346952381184No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC22300.10851766331786672No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC21460.10443000245746278No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC21370.10399203879384808No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA20860.1015102447000314No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT20550.10000170319202517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAC450.003826819620.55555517
CGAATTA1401.87174E-917.17857215
GGTATCA19100.016.9502621
GCTTAGG11950.016.4100421
CTTATAC7100.015.63380237
CGGCCTT7450.015.39597324
CTTAGGA13450.015.2676582
TAGGACA13800.015.1485514
GTATTAG3300.015.1363641
TTAGGAC13650.014.9084263
GGACTAG1508.110328E-814.81
CGCTAGA750.004105869714.810
AATACCG750.004105869714.85
AGTGCTA3650.014.69863116
TAGAACA11000.014.6318184
ATCTCGC8150.014.52760911
CGTTTAG2600.014.23076926
TCGCCAT6500.013.94615513
TCCTCGG9050.013.90055320
GTAGAAC11050.013.8959273
Produced by FastQC (version 0.11.5)