FastQCFastQC Report
Fri 10 Feb 2017
ERR1630450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034382
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59320.2915873223416251No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58860.2893261934091041No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41540.20418977360200785No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28920.14215619288806133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCG500.00703669818.58
GGTATCA28900.017.0276811
GACGGAC3100.016.7096797
ACAGCGT904.4486645E-516.4444458
CGAACGA1708.54925E-1116.3235316
GTATTAC1659.749783E-1015.696971
ACGGACC3450.015.5507258
GGGTGTA1557.21775E-915.51612957
AAGACGG3850.015.3766235
CGGACCA3650.015.2054819
CCTAGTA1803.3342076E-914.3888882
GTATACT1702.4394467E-814.1470594
CTTATAC8150.014.07361937
GTCTTAC1203.3028147E-513.8750011
CGCAAGA4050.013.7037042
CAAGACG4200.013.6547614
TGCGTAC950.001245650213.63157916
TCTTATA14550.013.60481137
ACGAACG2051.4260877E-913.53658515
TAGGACA6200.013.427424
Produced by FastQC (version 0.11.5)