##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630450.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2034382
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83451878752368	33.0	31.0	34.0	30.0	34.0
2	32.04962489837209	33.0	31.0	34.0	30.0	34.0
3	32.17514999641168	34.0	31.0	34.0	30.0	34.0
4	35.815445673428094	37.0	35.0	37.0	35.0	37.0
5	35.74328518439506	37.0	35.0	37.0	35.0	37.0
6	35.79550988948978	37.0	35.0	37.0	35.0	37.0
7	35.77107593362505	37.0	35.0	37.0	35.0	37.0
8	35.779769974370595	37.0	35.0	37.0	35.0	37.0
9	37.52116809920654	39.0	37.0	39.0	35.0	39.0
10	37.41397485821247	39.0	37.0	39.0	34.0	39.0
11	37.4861063458092	39.0	37.0	39.0	35.0	39.0
12	37.416826338416286	39.0	37.0	39.0	34.0	39.0
13	37.42004500629675	39.0	37.0	39.0	34.0	39.0
14	38.65784891922952	40.0	38.0	41.0	34.0	41.0
15	38.666276048451074	40.0	38.0	41.0	34.0	41.0
16	38.6132304552439	40.0	38.0	41.0	34.0	41.0
17	38.591925705201874	40.0	38.0	41.0	34.0	41.0
18	38.55537504755744	40.0	38.0	41.0	34.0	41.0
19	38.58479626736768	40.0	38.0	41.0	34.0	41.0
20	38.571595206799905	40.0	38.0	41.0	34.0	41.0
21	38.51147719553162	40.0	38.0	41.0	34.0	41.0
22	38.478472577913095	40.0	38.0	41.0	34.0	41.0
23	38.42076561825655	40.0	38.0	41.0	34.0	41.0
24	38.386061221540494	40.0	38.0	41.0	34.0	41.0
25	38.359169025286306	40.0	38.0	41.0	34.0	41.0
26	38.19442120506375	40.0	38.0	41.0	33.0	41.0
27	38.0788947208538	40.0	38.0	41.0	33.0	41.0
28	38.00633804270781	40.0	37.0	41.0	33.0	41.0
29	37.95016520987701	40.0	37.0	41.0	33.0	41.0
30	37.88379026161262	40.0	37.0	41.0	33.0	41.0
31	37.8202338597176	40.0	37.0	41.0	33.0	41.0
32	37.72276937173058	40.0	37.0	41.0	33.0	41.0
33	37.634317448738734	40.0	37.0	41.0	32.0	41.0
34	37.56937733424696	40.0	37.0	41.0	32.0	41.0
35	37.438898397646064	40.0	37.0	41.0	32.0	41.0
36	37.349937229094635	40.0	36.0	41.0	31.0	41.0
37	37.28129476175074	40.0	36.0	41.0	31.0	41.0
38	37.166746461578995	40.0	36.0	41.0	31.0	41.0
39	37.08313187985344	39.0	36.0	41.0	31.0	41.0
40	36.97967343399617	39.0	36.0	41.0	31.0	41.0
41	36.855245475038615	39.0	36.0	41.0	30.0	41.0
42	36.808886433324716	39.0	35.0	41.0	30.0	41.0
43	35.60138459738633	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	3.0
11	3.0
12	5.0
13	4.0
14	1.0
15	6.0
16	17.0
17	50.0
18	83.0
19	176.0
20	372.0
21	661.0
22	1257.0
23	2108.0
24	3361.0
25	5280.0
26	8151.0
27	11687.0
28	16829.0
29	23547.0
30	31648.0
31	41483.0
32	53189.0
33	68582.0
34	90676.0
35	121080.0
36	169055.0
37	256298.0
38	470584.0
39	658181.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.91469448707274	19.77637434857367	12.666500195145257	25.64243096920834
2	18.324926193802344	21.798413473969	34.63789003245212	25.23877029977654
3	19.617702083482847	23.502075814670008	30.471759974282115	26.40846212756503
4	14.707906381397398	16.18565244875348	34.97253711446523	34.133904055383894
5	14.483071517541937	36.602122905137776	34.33912608349858	14.575679493821712
6	33.66825896021495	35.64099564388596	16.01267608541562	14.67806931048348
7	28.171159595395558	31.149852879154455	21.945878404350804	18.733109121099183
8	26.568019182238146	33.79920781839399	20.654134769182974	18.97863823018489
9	26.4573221744982	14.464146851476272	20.501115326423456	38.57741564760207
10	16.72596395367242	27.030174274054726	33.64289499218928	22.600966780083585
11	34.61729409717545	22.365416131287045	21.903752589238405	21.113537182299098
12	20.710712147472794	25.973588047869082	29.899448579470327	23.416251225187796
13	29.398854295800884	20.162732466174003	25.642332659254752	24.796080578770358
14	22.469624682090185	21.64731107530444	25.604286707216247	30.278777535389125
15	24.845186400587497	28.095903325924038	22.835878414181803	24.22303185930666
16	24.369956084943734	27.059224865339942	24.660658617703067	23.91016043201326
17	23.04434467076488	26.993111421552097	26.213808419461042	23.748735488221975
18	22.981327990515055	25.88968050248183	27.343193166278507	23.785798340724604
19	23.666155127208164	25.999738495523456	27.445730447870652	22.88837592939772
20	24.24642962826057	25.472600524385292	27.023931592001894	23.25703825535224
21	24.138534454197885	25.938491394438213	26.290686803166764	23.632287348197142
22	24.007290666158077	25.836593127544383	26.586943848303807	23.56917235799373
23	23.450807173873933	25.87286950041831	26.720055525461788	23.95626780024597
24	23.649393280121433	26.228063362731284	26.686974226079467	23.435569131067812
25	24.080875666418596	25.652999289219036	26.585075959185637	23.681049085176728
26	23.579199973259694	26.4322039813565	26.505494051756255	23.483101993627546
27	24.027886601434737	25.906049109754214	26.225851388775563	23.84021290003549
28	23.40317600136061	26.15968879001092	26.92414698911021	23.51298821951826
29	23.119207700422045	26.35075418480895	27.029486104379608	23.5005520103894
30	23.203508485623644	26.992226631969807	26.699951139953065	23.104313742453485
31	23.766185504983824	26.394403804201964	26.44940822323438	23.390002467579833
32	23.033530575870216	26.219068001977995	26.787102913808713	23.960298508343076
33	23.07555808102903	26.11343395684783	26.99424198601836	23.816765976104783
34	23.742836891006704	25.824697623160255	26.764147539645943	23.6683179461871
35	23.683261059132455	26.018073301867595	27.24994617530041	23.04871946369954
36	23.130218415223887	26.2798235532953	26.851446778431974	23.738511253048838
37	23.862480104523144	25.3882505842069	27.014542991434254	23.734726319835705
38	23.16634732316743	25.698320177823042	27.550971253186475	23.584361245823054
39	23.299950550093346	25.625423347237636	27.522313901715606	23.55231220095341
40	22.848609553171432	25.454806422785886	28.17469875372472	23.521885270317963
41	22.289815776977974	25.54092594212886	28.42637223490967	23.742886045983497
42	22.917229900775766	25.40112918812691	28.057169204210417	23.624471706886908
43	22.47837426795951	25.03777559966614	28.341776519847308	24.14207361252705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	251.0
1	272.5
2	294.0
3	678.5
4	1063.0
5	1063.0
6	1559.5
7	2056.0
8	1925.0
9	1794.0
10	2480.0
11	3166.0
12	3166.0
13	5687.0
14	8208.0
15	11908.0
16	15608.0
17	15106.0
18	14604.0
19	14604.0
20	18622.5
21	22641.0
22	23374.0
23	24107.0
24	28958.0
25	33809.0
26	33809.0
27	37990.5
28	42172.0
29	49026.5
30	55881.0
31	62622.0
32	69363.0
33	69363.0
34	76256.0
35	83149.0
36	91449.5
37	99750.0
38	105763.0
39	111776.0
40	111776.0
41	118230.0
42	124684.0
43	123363.5
44	122043.0
45	126565.5
46	131088.0
47	131088.0
48	133374.0
49	135660.0
50	138234.5
51	140809.0
52	146923.0
53	153037.0
54	153037.0
55	143239.5
56	133442.0
57	127264.0
58	121086.0
59	109737.5
60	98389.0
61	98389.0
62	90278.5
63	82168.0
64	72698.0
65	63228.0
66	54660.0
67	46092.0
68	46092.0
69	39470.5
70	32849.0
71	28657.0
72	24465.0
73	19853.0
74	15241.0
75	15241.0
76	11600.5
77	7960.0
78	6672.0
79	5384.0
80	4171.0
81	2958.0
82	2958.0
83	2225.0
84	1492.0
85	1281.5
86	1071.0
87	961.0
88	851.0
89	851.0
90	709.0
91	567.0
92	324.5
93	82.0
94	56.5
95	31.0
96	31.0
97	20.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2034382.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.71480798385354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10327551025709	42.2262878740092
2	12.033025960442389	12.686373059388908
3	3.4616357107276006	5.474383854031675
4	1.3524401787375013	2.8517449732718374
5	0.734024613686723	1.9346983282958943
6	0.4372476042884556	1.3829654100879547
7	0.28131241592138295	1.0380530992138752
8	0.20089273947335407	0.847201774935054
9	0.14468110915292628	0.6864153199098718
>10	0.9939234233127292	10.284515195586831
>50	0.13427188696993195	4.97586015838191
>100	0.1145918639315022	11.712487958476881
>500	0.008023876843924218	2.8063056882378095
>1k	4.665044676700127E-4	0.5114580400992846
>5k	1.866017870680051E-4	0.581249266073021
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5932	0.2915873223416251	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5886	0.2893261934091041	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4154	0.20418977360200785	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2892	0.14215619288806133	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.9154976793935454E-5	0.0
6	0.0	0.0	0.0	4.9154976793935454E-5	0.0
7	0.0	0.0	0.0	4.9154976793935454E-5	0.0
8	0.0	0.0	0.0	4.9154976793935454E-5	0.0
9	0.0	4.9154976793935454E-5	0.0	4.9154976793935454E-5	0.0
10	0.0	9.830995358787091E-5	0.0	4.9154976793935454E-5	0.0
11	0.0	9.830995358787091E-5	0.0	9.830995358787091E-5	0.0
12	0.0	9.830995358787091E-5	0.0	1.4746493038180636E-4	0.0
13	0.0	9.830995358787091E-5	0.0	1.4746493038180636E-4	0.0
14	0.0	9.830995358787091E-5	0.0	2.457748839696773E-4	0.0
15	0.0	9.830995358787091E-5	0.0	3.440848375575482E-4	0.0
16	0.0	9.830995358787091E-5	0.0	4.423947911454191E-4	0.0
17	0.0	9.830995358787091E-5	0.0	6.390146983211609E-4	0.0
18	0.0	9.830995358787091E-5	0.0	8.356346054969028E-4	0.0
19	0.0	9.830995358787091E-5	0.0	0.0011305644662605156	0.0
20	0.0	9.830995358787091E-5	0.0	0.00162211423419987	0.0
21	0.0	9.830995358787091E-5	0.0	0.0024577488396967727	0.0
22	0.0	9.830995358787091E-5	0.0	0.0036866232595451592	0.0
23	0.0	9.830995358787091E-5	0.0	0.005800287261684384	0.0
24	0.0	9.830995358787091E-5	0.0	0.008307191078175092	0.0
25	0.0	9.830995358787091E-5	0.0	0.009929305312374962	0.0
26	0.0	9.830995358787091E-5	0.0	0.012927758896805025	0.0
27	0.0	9.830995358787091E-5	0.0	0.023938473698646566	0.0
28	0.0	9.830995358787091E-5	0.0	0.04738539762935378	0.0
29	0.0	9.830995358787091E-5	0.0	0.08100740175640563	0.0
30	0.0	9.830995358787091E-5	0.0	0.13080139324866225	0.0
31	0.0	9.830995358787091E-5	0.0	0.2791511132127594	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTCG	50	0.007036698	18.5	8
GGTATCA	2890	0.0	17.027681	1
GACGGAC	310	0.0	16.709679	7
ACAGCGT	90	4.4486645E-5	16.444445	8
CGAACGA	170	8.54925E-11	16.32353	16
GTATTAC	165	9.749783E-10	15.69697	1
ACGGACC	345	0.0	15.550725	8
GGGTGTA	155	7.21775E-9	15.5161295	7
AAGACGG	385	0.0	15.376623	5
CGGACCA	365	0.0	15.205481	9
CCTAGTA	180	3.3342076E-9	14.388888	2
GTATACT	170	2.4394467E-8	14.147059	4
CTTATAC	815	0.0	14.073619	37
GTCTTAC	120	3.3028147E-5	13.875001	1
CGCAAGA	405	0.0	13.703704	2
CAAGACG	420	0.0	13.654761	4
TGCGTAC	95	0.0012456502	13.631579	16
TCTTATA	1455	0.0	13.604811	37
ACGAACG	205	1.4260877E-9	13.536585	15
TAGGACA	620	0.0	13.42742	4
>>END_MODULE