FastQCFastQC Report
Fri 10 Feb 2017
ERR1630449.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630449.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962215
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG37400.19060092803286083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34710.1768919308026898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30730.1566087304398346No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT28280.14412284076923274No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG27050.1378544145264408No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24600.12536852485583894No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC23730.12093475995240072No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA22320.1137490030399319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.018.2565781
AACCGCG1900.017.5263167
AATACCG650.001580472917.0769215
TCGAACG1107.814524E-716.8181823
CGGCCTT7400.015.7524
TAGGACA11050.015.73755554
TCTAGCG1455.351285E-815.31034528
ATCTCGC7350.015.1020411
GCTTAGG9350.014.8395721
GTATTAG3250.014.81
TACTCGC750.00410578114.85
AACACCG750.00410578114.85
GAACCGC2800.014.5357156
CGTTTAG1551.2113742E-714.3225826
CGAACGT1304.447533E-614.2307684
TTCCTCG8350.014.17964119
TTAGGAC11150.014.1031393
TCTCGCC8150.014.07361912
AAAGCCG3200.013.87515
ACGGACC2551.8189894E-1213.7843158
Produced by FastQC (version 0.11.5)