FastQCFastQC Report
Fri 10 Feb 2017
ERR1630448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962742
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG85750.2894278340807266No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT65270.22030267907229178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62930.2124045900723046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59580.20109749684582728No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG52840.1783483003244967No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC49000.16538733376041517No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46800.15796177999974348No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA45910.15495780597838082No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC39610.1336937202091846No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC38670.13052098360235215No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC38190.12890086278184196No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA38000.12825956495705668No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT37070.12512058086731817No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT35890.12113778385023062No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC34530.11654744152545175No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC33930.11452229049981402No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA33150.11188959416648496No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC32690.1103369783801627No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT31540.10645543891435703No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC30690.10358647496137026No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA30240.10206761169214194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29730.10034623332034988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG909.490941E-820.55555735
GACGGAC2900.019.7758627
TAACGGC1002.8787326E-718.536
GGTATCA26200.018.0763361
ACGGACC3300.017.9393948
AAGACGG3550.016.6760565
CGGCCTT12650.016.67193824
GCGAAAG3600.015.93055618
GCTTAGG23350.015.8458251
CGGACCA3550.015.6338029
TCGGCCT14150.015.42756223
TTCCTCG14300.015.39510619
ATCTCGC13950.015.25089611
CGTAATG855.367698E-415.2352942
TCGCCAT11450.014.8646313
CTTAGGA24950.014.8296592
TCCTCGG14850.014.82491620
TAGAACA19250.014.84
GCGCAAG4150.014.7108431
CGCAAGA4150.014.7108432
Produced by FastQC (version 0.11.5)