FastQCFastQC Report
Fri 10 Feb 2017
ERR1630447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465643
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33130.7114892739716908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29350.6303112040769431No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18600.3994476455138379No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12640.27145259351047907No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT10910.23429966734171886No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT7340.1576314902189016No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA7210.154839651836278No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA6620.14216900071514013No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC6540.14045094632583333No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG6520.14002143272850662No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG6290.13508202635924946No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC6190.13293445837261594No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG6150.1320754311779625No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG6040.12971310639266562No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG5910.12692126801004203No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5870.1260622408153886No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG5680.1219818616407849No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG5390.11575391447954764No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG5360.11510964408355759No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA5330.11446537368756751No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.11339158969425076No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA5070.10888169692232033No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC5070.10888169692232033No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA4780.10265374976108307No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC4720.10136520896910295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG358.861441E-426.428575
GTATCGC358.861441E-426.428576
CTAGGAG358.861441E-426.428573
TACACTA509.0759295E-625.9000025
TCGTGGT400.001929471623.12535
ACAGAGG450.003822441720.5555558
TTTGCGT450.003822441720.5555553
TTATACA1457.2759576E-1219.1379337
CGAACTA500.007028751518.524
TAACGCT500.007028751518.532
ATCGTGG500.007028751518.534
ATACTGG500.007028751518.56
GATGCGT701.2172217E-418.56
CTGATCA500.007028751518.59
AAGACGG2450.018.1224485
GAGCTAT2500.017.7632
GAACCGC1054.782123E-717.6190476
CAAGACG2750.017.490914
GAATAAA852.7174861E-517.4117641
TTGAACG852.7174861E-517.41176410
Produced by FastQC (version 0.11.5)