FastQCFastQC Report
Fri 10 Feb 2017
ERR1630446.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630446.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171124
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62720.28888262485238064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61310.2823882928842388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49090.2261040824936761No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG43680.20118611373647932No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT32400.14923145799134457No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG32210.14835633524386446No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG31250.1439346624144913No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC29200.13449254856010068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.1165755617827448No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC24370.11224600713731689No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC23830.10975881617079448No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA23060.10621226608890141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA358.8719476E-426.4285710
GCGCTAG400.00193173723.12500211
GGTATCA21500.019.6186051
GCCGTTA701.2198312E-418.515
TACGACG2550.016.6862745
GACGGAC2700.016.4444457
CGACGGT2650.016.0566047
ACGACGG2650.016.0566046
CGCAATA2750.015.47272836
ACGGTAT2750.015.4727289
AAGACGG3450.015.0144925
TGTATCG750.004105970314.7999995
GAACCGA750.004105970314.7999996
CGATACC750.004105970314.7999994
TCTGTCG1406.001428E-714.5357148
CGGACCA3200.014.4531269
CTTATAC7300.014.44520737
GACGGTA2950.014.4237298
CTAGCGG2700.014.38888929
TTGCGCG1551.2115925E-714.32258118
Produced by FastQC (version 0.11.5)