##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630446.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2171124
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.825402878877483	33.0	31.0	34.0	30.0	34.0
2	32.035379830907864	33.0	31.0	34.0	30.0	34.0
3	32.17246136102774	34.0	31.0	34.0	30.0	34.0
4	35.813264005188095	37.0	35.0	37.0	35.0	37.0
5	35.746803959608016	37.0	35.0	37.0	35.0	37.0
6	35.795020920039576	37.0	35.0	37.0	35.0	37.0
7	35.76400334573244	37.0	35.0	37.0	33.0	37.0
8	35.76565364299782	37.0	35.0	37.0	33.0	37.0
9	37.519672298772434	39.0	37.0	39.0	35.0	39.0
10	37.40647655315864	39.0	37.0	39.0	34.0	39.0
11	37.481706249850305	39.0	37.0	39.0	35.0	39.0
12	37.378126260867646	39.0	37.0	39.0	34.0	39.0
13	37.398930692120764	39.0	37.0	39.0	34.0	39.0
14	38.638707876657435	40.0	38.0	41.0	34.0	41.0
15	38.640618868383385	40.0	38.0	41.0	34.0	41.0
16	38.60987350330981	40.0	38.0	41.0	34.0	41.0
17	38.56592069361308	40.0	38.0	41.0	34.0	41.0
18	38.52620347801415	40.0	38.0	41.0	34.0	41.0
19	38.55998505843056	40.0	38.0	41.0	34.0	41.0
20	38.54529727459141	40.0	38.0	41.0	34.0	41.0
21	38.48837284282243	40.0	38.0	41.0	34.0	41.0
22	38.4488329547276	40.0	38.0	41.0	34.0	41.0
23	38.39974824100328	40.0	38.0	41.0	34.0	41.0
24	38.363702395625495	40.0	38.0	41.0	34.0	41.0
25	38.34624507858602	40.0	38.0	41.0	34.0	41.0
26	38.19215853170984	40.0	38.0	41.0	33.0	41.0
27	38.066010048251506	40.0	38.0	41.0	33.0	41.0
28	38.015094485621276	40.0	37.0	41.0	33.0	41.0
29	37.95274429281791	40.0	37.0	41.0	33.0	41.0
30	37.88606546655097	40.0	37.0	41.0	33.0	41.0
31	37.811290373097066	40.0	37.0	41.0	33.0	41.0
32	37.7171036753313	40.0	37.0	41.0	33.0	41.0
33	37.635241469395574	40.0	37.0	41.0	32.0	41.0
34	37.55165757460191	40.0	37.0	41.0	32.0	41.0
35	37.44280105604286	40.0	37.0	41.0	32.0	41.0
36	37.352825541056156	40.0	36.0	41.0	31.0	41.0
37	37.27840326024677	40.0	36.0	41.0	31.0	41.0
38	37.19549136760498	39.0	36.0	41.0	31.0	41.0
39	37.086878962233385	39.0	36.0	41.0	31.0	41.0
40	36.98633841273	39.0	36.0	41.0	31.0	41.0
41	36.864438880506135	39.0	35.0	41.0	30.0	41.0
42	36.8192788620088	39.0	35.0	41.0	30.0	41.0
43	35.623229258209115	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	6.0
11	6.0
12	5.0
13	2.0
14	3.0
15	9.0
16	14.0
17	36.0
18	71.0
19	134.0
20	297.0
21	572.0
22	1094.0
23	2101.0
24	3355.0
25	5349.0
26	8409.0
27	12448.0
28	17806.0
29	24849.0
30	33233.0
31	44334.0
32	57685.0
33	73958.0
34	98465.0
35	131165.0
36	184530.0
37	280720.0
38	499994.0
39	690470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71560537306943	19.171175851770787	11.659813073781137	25.45340570137864
2	18.556194855752135	21.287913541557277	34.39043555319733	25.765456049493256
3	19.899277977674238	22.698657469587182	29.5887752150499	27.81328933768868
4	13.631004032934094	15.79872913753429	35.57005495770854	35.00021187182307
5	14.620353328506342	36.53181485718918	34.199658794246666	14.648173020057815
6	34.60613028090519	35.70749528815489	15.915857408420708	13.77051702251921
7	30.110716845283825	30.497567158762006	20.890884168753143	18.500831827201026
8	27.894952107756165	33.15946026113662	20.081027154598264	18.86456047650894
9	26.849134365425463	13.545978949152603	19.6343921397396	39.97049454568233
10	16.489200985296097	26.72583417621472	33.69365360983527	23.091311228653915
11	36.130778343383426	21.95406618875753	21.054808477083757	20.860346990775287
12	21.466392522951246	25.304865129766885	29.78314458317443	23.44559776410744
13	30.48858563582734	19.761100701756327	24.90585521600793	24.844458446408403
14	22.82877440440988	20.699831055250645	25.350325453543874	31.121069086795593
15	24.752662676106937	27.920560962892953	22.943185188869915	24.383591172130195
16	25.483758642988608	26.275284138538378	23.757648112222057	24.483309106250957
17	23.55650805757755	26.231067410244645	25.39403553182591	24.818389000351893
18	23.280153505741726	24.951591894336758	27.206967450960885	24.56128714896063
19	24.894478620290688	25.408083554877564	26.585998772985793	23.111439051845956
20	25.175162726771937	25.18603267247748	26.153181485718918	23.48562311503166
21	24.806782109174787	25.384961890707302	25.675226288318864	24.13302971179905
22	25.37975721331439	24.626138350458106	26.17367778164674	23.82042665458076
23	23.553099684771574	25.33411265316951	26.307110971091475	24.80567669096744
24	24.043398718820296	25.79497992744772	26.078980288551	24.082641065180983
25	24.42380075942231	25.435488714601284	26.036513805752225	24.104196720224177
26	24.680488078985814	25.51102562543641	25.488733024921657	24.319753270656122
27	24.65363562836577	25.105291084249448	25.5721460404841	24.668927246900687
28	24.163474771592963	25.42682960531043	26.31927057137225	24.09042505172436
29	23.992549481282506	25.978847822602486	26.687144538957703	23.34145815715731
30	24.261350342034817	25.755507285627168	25.706776766320118	24.276365606017897
31	23.879151996845874	25.53769383968857	26.16925610881737	24.413898054648193
32	23.37222563059503	25.967655463253138	26.016385982560188	24.643732923591653
33	23.73678334355845	25.11243024350521	26.548921203947817	24.601865208988524
34	24.644515928155187	25.01819334132919	26.722978512512412	23.614312218003207
35	24.602924568103894	25.305417838870557	26.017076868939775	24.074580724085774
36	23.606896704195616	25.793598154688542	25.555841121925788	25.04366401919006
37	23.87316431488943	25.326144430258246	26.974875686510767	23.82581556834156
38	23.552915448403684	25.15825904001798	26.33207499894064	24.956750512637694
39	23.333443875154067	25.565052940320314	27.131384481033788	23.97011870349183
40	24.029120400308777	24.828015350574173	26.961242195286868	24.181622053830182
41	23.030190813606225	25.24567919658205	27.484197125544192	24.23993286426754
42	23.098496447001647	25.80165849578375	27.052853729220438	24.046991327994164
43	23.41050073602429	24.156565907797066	27.125074385433535	25.307858970745105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	292.0
1	293.0
2	294.0
3	673.5
4	1053.0
5	1053.0
6	1452.0
7	1851.0
8	1754.0
9	1657.0
10	2318.0
11	2979.0
12	2979.0
13	5085.5
14	7192.0
15	10649.0
16	14106.0
17	14176.5
18	14247.0
19	14247.0
20	17407.0
21	20567.0
22	21362.0
23	22157.0
24	26411.5
25	30666.0
26	30666.0
27	34945.0
28	39224.0
29	45365.5
30	51507.0
31	58473.0
32	65439.0
33	65439.0
34	78088.5
35	90738.0
36	97829.0
37	104920.0
38	107634.0
39	110348.0
40	110348.0
41	114458.0
42	118568.0
43	124509.5
44	130451.0
45	129988.5
46	129526.0
47	129526.0
48	131504.5
49	133483.0
50	137022.5
51	140562.0
52	149851.5
53	159141.0
54	159141.0
55	158809.5
56	158478.0
57	147841.5
58	137205.0
59	133060.5
60	128916.0
61	128916.0
62	118228.0
63	107540.0
64	89460.5
65	71381.0
66	62601.0
67	53821.0
68	53821.0
69	47244.5
70	40668.0
71	39033.5
72	37399.0
73	32584.5
74	27770.0
75	27770.0
76	17421.0
77	7072.0
78	5479.0
79	3886.0
80	3150.0
81	2414.0
82	2414.0
83	1883.5
84	1353.0
85	1148.0
86	943.0
87	813.5
88	684.0
89	684.0
90	587.5
91	491.0
92	290.5
93	90.0
94	60.5
95	31.0
96	31.0
97	19.0
98	7.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2171124.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.312678287989016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.12055589981657	47.63861679404995
2	10.989988007614132	12.597312941384924
3	2.754517357904203	4.736063015167353
4	0.986630443682506	2.2618573283164536
5	0.5159132252591555	1.4784184351898393
6	0.31200547574336435	1.0729121673222242
7	0.20196071123819043	0.8102436489005499
8	0.15160159303377446	0.6950954663593083
9	0.10178991773625462	0.5250467527360946
>10	0.7069595488295444	7.93841992943994
>50	0.07934591197800293	3.1421295611407456
>100	0.062393502225125026	7.194950688376303
>500	0.010382533187986692	3.9785833493635256
>1k	0.00579490224445768	5.358224737824103
>5k	1.6096950679049112E-4	0.5721251844286531
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6272	0.28888262485238064	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6131	0.2823882928842388	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4909	0.2261040824936761	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	4368	0.20118611373647932	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	3240	0.14923145799134457	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	3221	0.14835633524386446	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	3125	0.1439346624144913	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	2920	0.13449254856010068	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2531	0.1165755617827448	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	2437	0.11224600713731689	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	2383	0.10975881617079448	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	2306	0.10621226608890141	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.6059091972637216E-5	0.0
6	0.0	0.0	0.0	4.6059091972637216E-5	0.0
7	0.0	0.0	0.0	4.6059091972637216E-5	0.0
8	0.0	0.0	0.0	4.6059091972637216E-5	0.0
9	0.0	0.0	0.0	9.211818394527443E-5	0.0
10	0.0	0.0	0.0	1.8423636789054886E-4	0.0
11	0.0	0.0	0.0	2.7635455183582327E-4	0.0
12	0.0	0.0	0.0	4.145318277537349E-4	0.0
13	0.0	0.0	0.0	4.6059091972637214E-4	0.0
14	0.0	0.0	0.0	5.527091036716465E-4	0.0
15	0.0	0.0	0.0	6.44827287616921E-4	4.6059091972637216E-5
16	0.0	0.0	0.0	7.369454715621955E-4	4.6059091972637216E-5
17	0.0	0.0	0.0	8.751227474801071E-4	4.6059091972637216E-5
18	4.6059091972637216E-5	0.0	0.0	8.751227474801071E-4	4.6059091972637216E-5
19	4.6059091972637216E-5	0.0	0.0	0.001059359115370656	4.6059091972637216E-5
20	4.6059091972637216E-5	0.0	0.0	0.0012435954832612048	4.6059091972637216E-5
21	4.6059091972637216E-5	0.0	0.0	0.0014278318511517537	4.6059091972637216E-5
22	4.6059091972637216E-5	0.0	0.0	0.0018423636789054885	4.6059091972637216E-5
23	4.6059091972637216E-5	0.0	0.0	0.0029017227942761447	4.6059091972637216E-5
24	4.6059091972637216E-5	0.0	0.0	0.004559850105291084	4.6059091972637216E-5
25	4.6059091972637216E-5	0.0	0.0	0.005112559208962731	4.6059091972637216E-5
26	4.6059091972637216E-5	0.0	0.0	0.006586450152087121	9.211818394527443E-5
27	4.6059091972637216E-5	0.0	0.0	0.013311077580092156	1.3817727591791164E-4
28	4.6059091972637216E-5	0.0	0.0	0.0300765870581321	1.3817727591791164E-4
29	4.6059091972637216E-5	0.0	0.0	0.05633026948253531	1.3817727591791164E-4
30	4.6059091972637216E-5	0.0	0.0	0.09138123847371224	1.8423636789054886E-4
31	4.6059091972637216E-5	0.0	0.0	0.19980434097730024	1.8423636789054886E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGCTA	35	8.8719476E-4	26.42857	10
GCGCTAG	40	0.001931737	23.125002	11
GGTATCA	2150	0.0	19.618605	1
GCCGTTA	70	1.2198312E-4	18.5	15
TACGACG	255	0.0	16.686274	5
GACGGAC	270	0.0	16.444445	7
CGACGGT	265	0.0	16.056604	7
ACGACGG	265	0.0	16.056604	6
CGCAATA	275	0.0	15.472728	36
ACGGTAT	275	0.0	15.472728	9
AAGACGG	345	0.0	15.014492	5
TGTATCG	75	0.0041059703	14.799999	5
GAACCGA	75	0.0041059703	14.799999	6
CGATACC	75	0.0041059703	14.799999	4
TCTGTCG	140	6.001428E-7	14.535714	8
CGGACCA	320	0.0	14.453126	9
CTTATAC	730	0.0	14.445207	37
GACGGTA	295	0.0	14.423729	8
CTAGCGG	270	0.0	14.388889	29
TTGCGCG	155	1.2115925E-7	14.322581	18
>>END_MODULE