FastQCFastQC Report
Fri 10 Feb 2017
ERR1630445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412011
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG9220.2237804330466905No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG6530.15849091407753677No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT6040.1465980277225608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGTC502.6980275E-422.1999994
CGTTATT450.003821705720.5555552
TATAAGG450.003821705720.5555552
GCGTTAT555.135399E-420.1818181
GTGTAAG555.135399E-420.1818181
GCTTAGG2350.018.8936161
TAGGACA2650.018.8490564
AACCGCG609.22311E-418.535
TCTAGGA609.22311E-418.52
AAACGGC609.22311E-418.522
AACGGCT609.22311E-418.523
CAATAGA701.2167899E-418.51
AAGTACC500.007027412318.49999837
AGGACCG500.007027412318.4999985
CGTAGTT500.007027412318.4999982
CGCAATA500.007027412318.4999982
TCGGTCA500.007027412318.4999984
CTTAGGA2650.018.1509442
TTGGCCG953.595038E-617.52631631
AATGCAG752.0632127E-417.2666665
Produced by FastQC (version 0.11.5)