##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630445.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412011
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.746851419015513	31.0	31.0	34.0	30.0	34.0
2	31.970284773950212	33.0	31.0	34.0	30.0	34.0
3	32.090048566664485	34.0	31.0	34.0	30.0	34.0
4	35.747086849622946	37.0	35.0	37.0	33.0	37.0
5	35.67482178873865	37.0	35.0	37.0	33.0	37.0
6	35.738167184856714	37.0	35.0	37.0	33.0	37.0
7	35.70735490071867	37.0	35.0	37.0	33.0	37.0
8	35.70735490071867	37.0	35.0	37.0	33.0	37.0
9	37.4610750683841	39.0	37.0	39.0	35.0	39.0
10	37.335059015414636	39.0	37.0	39.0	34.0	39.0
11	37.41763205351313	39.0	37.0	39.0	34.0	39.0
12	37.33135523080694	39.0	37.0	39.0	34.0	39.0
13	37.327731541148175	39.0	37.0	39.0	34.0	39.0
14	38.549812990429864	40.0	38.0	41.0	34.0	41.0
15	38.569574598736445	40.0	38.0	41.0	34.0	41.0
16	38.510493651868515	40.0	38.0	41.0	34.0	41.0
17	38.46485409370138	40.0	38.0	41.0	34.0	41.0
18	38.40434600047086	40.0	38.0	41.0	34.0	41.0
19	38.441078029470084	40.0	38.0	41.0	34.0	41.0
20	38.41564424250809	40.0	38.0	41.0	34.0	41.0
21	38.34934746887826	40.0	38.0	41.0	34.0	41.0
22	38.32115647397764	40.0	38.0	41.0	34.0	41.0
23	38.25726254881545	40.0	38.0	41.0	34.0	41.0
24	38.23032880190092	40.0	38.0	41.0	33.0	41.0
25	38.2055284931713	40.0	38.0	41.0	33.0	41.0
26	38.03166420314021	40.0	38.0	41.0	33.0	41.0
27	37.90793935113383	40.0	37.0	41.0	33.0	41.0
28	37.83041472193703	40.0	37.0	41.0	33.0	41.0
29	37.78963911157712	40.0	37.0	41.0	33.0	41.0
30	37.72874025208065	40.0	37.0	41.0	32.0	41.0
31	37.68512976595285	40.0	37.0	41.0	32.0	41.0
32	37.606961950045026	40.0	37.0	41.0	32.0	41.0
33	37.5185808145899	40.0	37.0	41.0	32.0	41.0
34	37.46371577457884	40.0	37.0	41.0	31.0	41.0
35	37.33920939004056	40.0	37.0	41.0	31.0	41.0
36	37.26449779253466	39.0	36.0	41.0	31.0	41.0
37	37.20773474494613	39.0	36.0	41.0	31.0	41.0
38	37.099579865586115	39.0	36.0	41.0	31.0	41.0
39	37.0386907145683	39.0	36.0	41.0	31.0	41.0
40	36.93274694122244	39.0	36.0	41.0	30.0	41.0
41	36.82942445711401	39.0	35.0	41.0	30.0	41.0
42	36.81459232884559	39.0	35.0	41.0	30.0	41.0
43	35.51445956539995	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.0
16	5.0
17	11.0
18	14.0
19	31.0
20	69.0
21	136.0
22	260.0
23	460.0
24	737.0
25	1163.0
26	1767.0
27	2498.0
28	3588.0
29	4991.0
30	6756.0
31	8762.0
32	11353.0
33	14736.0
34	19205.0
35	25796.0
36	35400.0
37	52909.0
38	98279.0
39	123082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73890745635432	18.186164932489667	12.003320299700736	27.071607311455274
2	19.88296428978838	20.65308935926468	32.742329695080954	26.721616655865983
3	20.690224290128175	21.82029120581732	29.107232573887593	28.382251930166913
4	15.808558509360187	15.086247697270219	32.6554388110997	36.44975498226989
5	15.54642958561786	35.580118006558074	33.5923070015121	15.281145406311966
6	36.87134566795547	33.33284790940048	14.927756783192681	14.868049639451375
7	30.54044673564541	29.244607546885888	20.30916650283609	19.90577921463262
8	27.57669091359211	32.83043413889435	19.650688937916705	19.94218600959683
9	27.221117882774976	13.711041695488714	18.16771882304113	40.90012159869518
10	17.373322556922023	26.273813077806174	32.27146848021048	24.081395885061323
11	37.63297581860678	20.4458133399351	20.133928463075016	21.787282378383104
12	22.297705643781356	24.530170310986843	27.793918123545247	25.378205921686558
13	31.54964309205337	18.610182737839523	23.849605957122506	25.990568212984606
14	23.946690743693736	19.716706592785144	23.011278825079913	33.32532383844121
15	26.87962214601066	26.792731262029413	20.681001235403908	25.646645356556014
16	26.838118399751465	25.101271567992118	22.023683833684053	26.03692619857237
17	25.59761753933754	25.100786144059263	23.411753569686248	25.88984274691695
18	25.026030858399412	23.969505668537977	24.80127957748701	26.203183895575606
19	26.25439612049193	24.379203467868578	24.069745710672773	25.29665470096672
20	26.306579193273965	23.60859297445942	24.464395368084833	25.620432464181782
21	26.25051272902908	24.362699054151467	23.487965127144665	25.89882308967479
22	26.506573853610703	23.84863510925679	23.547186846953117	26.09760419017939
23	25.490581562142754	24.232362728179588	24.007611447267184	26.269444262410467
24	26.36701447291456	24.492064532257636	23.758103545779115	25.382817449048687
25	26.58351354696841	23.907371405132388	23.76150151330911	25.747613534590098
26	26.624289157328324	24.346680064367213	23.400346107264127	25.628684671040336
27	26.805352284283675	23.434568494530485	23.632136035202944	26.1279431859829
28	25.91921089485475	23.947176167626594	24.164160665613295	25.969452271905364
29	25.27408248808891	24.332602770314384	24.150811507459753	26.242503234136954
30	25.243743492285397	25.228452638400434	24.10008470647628	25.427719162837885
31	26.396625332818786	24.199596612711797	23.571458043595925	25.832320010873495
32	25.279907575283183	24.181878639162548	24.174354568203277	26.363859217350992
33	25.497620209169174	23.785044574052634	24.302021062544448	26.415314154233748
34	25.880619692192685	23.928730058178058	24.36366990201718	25.82698034761208
35	26.136923528740734	23.7665984646041	24.51002521777331	25.586452788881854
36	25.522376829744836	24.6940008883258	24.110521321032692	25.673100960896676
37	26.831807888624333	23.29646538563291	24.05396943285495	25.817757292887812
38	25.62310229581249	23.848392397290365	24.88622876573684	25.642276541160307
39	25.80634983046569	23.34719218661638	24.92870335986175	25.917754623056183
40	24.992779318998764	23.09113106203475	25.93935598806828	25.976733630898202
41	24.738659890148565	23.396948139734132	26.204640167374173	25.65975180274313
42	25.485727322814196	22.730703791889052	26.00076211557458	25.782806769722168
43	24.64327408734233	22.600852889850028	26.16459269291354	26.591280329894108
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	4.0
2	6.0
3	12.0
4	18.0
5	18.0
6	26.0
7	34.0
8	42.5
9	51.0
10	63.5
11	76.0
12	76.0
13	136.5
14	197.0
15	311.5
16	426.0
17	497.5
18	569.0
19	569.0
20	798.5
21	1028.0
22	1300.5
23	1573.0
24	2003.5
25	2434.0
26	2434.0
27	2915.5
28	3397.0
29	4664.0
30	5931.0
31	7012.0
32	8093.0
33	8093.0
34	9477.0
35	10861.0
36	12283.5
37	13706.0
38	16246.5
39	18787.0
40	18787.0
41	20369.5
42	21952.0
43	22211.0
44	22470.0
45	24831.5
46	27193.0
47	27193.0
48	29682.5
49	32172.0
50	33438.0
51	34704.0
52	36994.5
53	39285.0
54	39285.0
55	35718.0
56	32151.0
57	31001.5
58	29852.0
59	27639.5
60	25427.0
61	25427.0
62	23831.0
63	22235.0
64	20009.0
65	17783.0
66	15627.5
67	13472.0
68	13472.0
69	11531.0
70	9590.0
71	8225.0
72	6860.0
73	5521.5
74	4183.0
75	4183.0
76	3236.5
77	2290.0
78	1898.5
79	1507.0
80	1174.0
81	841.0
82	841.0
83	627.5
84	414.0
85	325.0
86	236.0
87	171.5
88	107.0
89	107.0
90	93.0
91	79.0
92	46.0
93	13.0
94	8.0
95	3.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	412011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.000423249237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.19566492962727	57.977559724228975
2	6.273129484284759	8.155121431515544
3	1.6001954297332237	3.1204014064246266
4	0.7090146465146383	1.8434500845343864
5	0.42052610809937124	1.3667187506906762
6	0.26700186405855103	1.0413140503284635
7	0.21212721118323954	0.9651850956713596
8	0.15120261946144545	0.786258740910982
9	0.11433974544661654	0.6688918663416086
>10	0.8693144523546583	11.680131579019832
>50	0.12461478758018495	5.580252843771268
>100	0.0617460659122405	6.284686487688831
>500	0.0011226557438589182	0.5300279388734745
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	922	0.2237804330466905	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	653	0.15849091407753677	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	604	0.1465980277225608	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	2.427119664280808E-4	0.0
17	0.0	0.0	0.0	4.854239328561616E-4	0.0
18	0.0	0.0	0.0	4.854239328561616E-4	0.0
19	0.0	0.0	0.0	4.854239328561616E-4	0.0
20	0.0	0.0	0.0	4.854239328561616E-4	0.0
21	0.0	0.0	0.0	7.281358992842424E-4	0.0
22	0.0	0.0	0.0	0.001213559832140404	0.0
23	0.0	0.0	0.0	0.0014562717985684849	0.0
24	0.0	0.0	0.0	0.002184407697852727	0.0
25	0.0	0.0	0.0	0.0029125435971369697	0.0
26	0.0	0.0	0.0	0.004611527362133536	0.0
27	0.0	0.0	0.0	0.010193902589979393	0.0
28	0.0	0.0	0.0	0.022329500911383433	0.0
29	0.0	0.0	0.0	0.04368815395705455	0.0
30	0.0	0.0	0.0	0.07257087796199616	0.0
31	0.0	0.0	0.0	0.16747125683537575	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGTC	50	2.6980275E-4	22.199999	4
CGTTATT	45	0.0038217057	20.555555	2
TATAAGG	45	0.0038217057	20.555555	2
GCGTTAT	55	5.135399E-4	20.181818	1
GTGTAAG	55	5.135399E-4	20.181818	1
GCTTAGG	235	0.0	18.893616	1
TAGGACA	265	0.0	18.849056	4
AACCGCG	60	9.22311E-4	18.5	35
TCTAGGA	60	9.22311E-4	18.5	2
AAACGGC	60	9.22311E-4	18.5	22
AACGGCT	60	9.22311E-4	18.5	23
CAATAGA	70	1.2167899E-4	18.5	1
AAGTACC	50	0.0070274123	18.499998	37
AGGACCG	50	0.0070274123	18.499998	5
CGTAGTT	50	0.0070274123	18.499998	2
CGCAATA	50	0.0070274123	18.499998	2
TCGGTCA	50	0.0070274123	18.499998	4
CTTAGGA	265	0.0	18.150944	2
TTGGCCG	95	3.595038E-6	17.526316	31
AATGCAG	75	2.0632127E-4	17.266666	5
>>END_MODULE