FastQCFastQC Report
Fri 10 Feb 2017
ERR1630444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296883
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC57450.4429852191755155No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT35970.2773573252174637No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG22570.17403266138888396No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT22330.17218207039493924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22090.17033147940099452No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG21430.16524235416764657No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG21270.1640086268383501No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG19850.15305929679084387No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT19060.14696776810244255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.1463509044377943No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT18910.1458111487312271No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG17510.13501603459988293No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT17430.1343991709352347No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG16540.12753656266602306No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG16110.1242209204685388No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG15910.1226787613069182No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC15420.11890047136094775No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT15360.11843782361246158No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC15300.1179751758639754No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT15230.1174354201574082No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG14980.11550772120538244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14820.11427399387608597No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT13270.10232226037352636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTT701.9233266E-723.78571312
CTATCGG400.001931319823.12500212
TTAGCGA502.7023608E-422.227
GGTATCA9700.021.7422681
GTATACT705.1027564E-621.1428574
TTAGACT1001.2902092E-820.354
GACAGGC13050.019.8467437
TGCATCA12950.019.5714314
GCTGCAT13400.019.32835812
ACAGGCT13500.019.3222228
CGTAGCC1153.0504452E-919.3043483
GCATCAG13200.019.20075815
CCAGGAC14850.018.6868693
CAGGACA15150.018.6831684
CTGCATC14450.017.79584713
GGACAGG15650.017.4952096
TTATACG752.0675235E-417.26666635
TATACGA752.0675235E-417.26666636
CGAACGA1301.3935278E-817.07692316
GTACGTA650.001580081517.07692313
Produced by FastQC (version 0.11.5)