##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630444.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1296883
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.777951442034478	31.0	31.0	34.0	30.0	34.0
2	32.004712838397914	33.0	31.0	34.0	30.0	34.0
3	32.12018663210174	34.0	31.0	34.0	30.0	34.0
4	35.77632677735771	37.0	35.0	37.0	35.0	37.0
5	35.704636424411454	37.0	35.0	37.0	33.0	37.0
6	35.75015942070333	37.0	35.0	37.0	35.0	37.0
7	35.72538232053316	37.0	35.0	37.0	33.0	37.0
8	35.724336736621574	37.0	35.0	37.0	33.0	37.0
9	37.47197549817524	39.0	37.0	39.0	35.0	39.0
10	37.363089037330276	39.0	37.0	39.0	34.0	39.0
11	37.42745721857716	39.0	37.0	39.0	34.0	39.0
12	37.351879853464034	39.0	37.0	39.0	34.0	39.0
13	37.34501647411524	39.0	37.0	39.0	34.0	39.0
14	38.573525907888374	40.0	38.0	41.0	34.0	41.0
15	38.580551213949136	40.0	38.0	41.0	34.0	41.0
16	38.54006568055869	40.0	38.0	41.0	34.0	41.0
17	38.498037216927045	40.0	38.0	41.0	34.0	41.0
18	38.392609048002015	40.0	38.0	41.0	34.0	41.0
19	38.420650128037764	40.0	38.0	41.0	34.0	41.0
20	38.391539560623436	40.0	38.0	41.0	34.0	41.0
21	38.34235393632271	40.0	38.0	41.0	34.0	41.0
22	38.2951083482473	40.0	38.0	41.0	34.0	41.0
23	38.236190928557164	40.0	38.0	41.0	34.0	41.0
24	38.18552174714296	40.0	38.0	41.0	33.0	41.0
25	38.15959573839737	40.0	38.0	41.0	33.0	41.0
26	37.962025872804254	40.0	37.0	41.0	33.0	41.0
27	37.84379469851945	40.0	37.0	41.0	33.0	41.0
28	37.75841922517297	40.0	37.0	41.0	32.0	41.0
29	37.68613668310866	40.0	37.0	41.0	32.0	41.0
30	37.623963765428336	40.0	37.0	41.0	32.0	41.0
31	37.57913705399793	40.0	37.0	41.0	32.0	41.0
32	37.50091719916137	40.0	37.0	41.0	32.0	41.0
33	37.3971622729267	40.0	37.0	41.0	31.0	41.0
34	37.31294419003102	40.0	36.0	41.0	31.0	41.0
35	37.16090271828685	39.0	36.0	41.0	31.0	41.0
36	37.06942337897868	39.0	36.0	41.0	31.0	41.0
37	36.9828303709741	39.0	36.0	41.0	30.0	41.0
38	36.871634526784604	39.0	36.0	41.0	30.0	41.0
39	36.794361557673284	39.0	35.0	41.0	30.0	41.0
40	36.659741857977934	39.0	35.0	41.0	30.0	41.0
41	36.52416833284113	39.0	35.0	41.0	30.0	41.0
42	36.482675769518146	39.0	35.0	40.0	30.0	41.0
43	35.17134699120892	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	0.0
11	2.0
12	2.0
13	0.0
14	2.0
15	6.0
16	11.0
17	37.0
18	72.0
19	134.0
20	316.0
21	593.0
22	996.0
23	1688.0
24	2781.0
25	4141.0
26	6212.0
27	9047.0
28	12280.0
29	16345.0
30	21881.0
31	28635.0
32	36217.0
33	46765.0
34	62836.0
35	82291.0
36	111076.0
37	165514.0
38	303282.0
39	383718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28694261548652	17.587939698492463	11.490705021193122	27.634412664827902
2	19.987616461932188	19.330348227249488	33.20091326665551	27.481122044162813
3	20.773886310484446	21.348494814104278	28.565568366614414	29.31205050879686
4	15.65044803579043	14.562917395015587	32.63386134292762	37.15277322626636
5	15.983245982868155	35.38869736128857	32.65822745768123	15.969829198162056
6	37.07134722253279	32.8318745792797	14.834645839293136	15.262132358894364
7	31.260799933378724	28.805990979911062	20.150776901231644	19.782432185478566
8	28.764815330295797	31.964949806574687	18.902938815606344	20.367296047523176
9	27.637034335402653	13.266115756008832	18.238576648780192	40.85827325980833
10	18.3588650633866	25.390725300586098	31.453030072874732	24.797379563152575
11	37.518419163486605	20.50693855960792	20.154478083219534	21.820164193685937
12	23.530572919839337	23.65055290261342	27.566634769674675	25.25223940787257
13	31.096559982666133	18.251530785737806	23.8197277626432	26.832181468952864
14	23.65880345412809	19.4404583913892	23.939090881752634	32.961647272730076
15	26.964652940936073	25.951145939919023	20.56083702230656	26.523364096838343
16	26.275462011607832	24.634913095475845	21.956259739698954	27.13336515321737
17	25.096943980297375	25.245531015519518	24.16031361348711	25.497211390696002
18	25.275834443045365	23.2506710320052	25.04412502901187	26.429369495937564
19	26.028793653706618	23.47497808206292	24.440215501321244	26.05601276290922
20	25.736014736872946	24.076034615304543	24.309131972583494	25.878818675239017
21	27.53517472277761	23.358236633528236	23.631121697177	25.47546694651715
22	27.000276817569514	23.78911590328503	23.913259715795487	25.29734756334997
23	26.199279349023776	23.9473414332673	23.68494305191756	26.168436165791363
24	26.306922058504895	23.90454651653233	24.00925912360637	25.779272301356404
25	26.33013155388728	23.492867128337714	24.331416172468913	25.845585145306092
26	26.685753456556995	24.115282565967785	24.27582133469249	24.923142642782732
27	27.162280637497755	23.6685190568463	23.287759959842173	25.881440345813772
28	25.607013123003387	23.14071508378165	24.94072325722521	26.311548535989754
29	25.554579711508286	23.50150321964279	25.129946186356054	25.81397088249287
30	25.358956821856715	23.995071259319463	24.779413408919694	25.86655850990413
31	25.65381765355857	23.9363921032198	24.43821069441114	25.971579548810492
32	25.262263442423098	23.69913091620447	24.649872039343563	26.388733602028864
33	26.034422534646534	23.883033396227724	24.301652500649634	25.780891568476104
34	26.573021621842525	23.95374139378803	24.019360266114987	25.45387671825446
35	26.722842384393967	23.637752981571968	24.69189587649773	24.947508757536337
36	26.145226670408974	24.387704981868062	23.25807339598098	26.208994951741982
37	25.53684488114965	23.682938245007453	24.59019047978885	26.19002639405405
38	26.282941483541695	23.58262079154403	24.74671963469334	25.38771809022094
39	24.923759506447382	24.002396515337157	24.841639531091086	26.232204447124374
40	25.776959062613976	22.87769983876726	25.89262099973552	25.452720098883248
41	24.44515041063843	23.37728229917425	26.152012170720102	26.025555119467214
42	25.05098763728108	23.80253268799113	25.09748373600394	26.048995938723845
43	25.01690591980927	21.99920887235009	26.389813113442003	26.594072094398648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	100.0
1	95.5
2	91.0
3	224.0
4	357.0
5	357.0
6	508.0
7	659.0
8	594.0
9	529.0
10	735.0
11	941.0
12	941.0
13	1797.0
14	2653.0
15	3575.5
16	4498.0
17	4442.5
18	4387.0
19	4387.0
20	5787.5
21	7188.0
22	7412.0
23	7636.0
24	9433.5
25	11231.0
26	11231.0
27	12977.0
28	14723.0
29	17457.0
30	20191.0
31	24013.0
32	27835.0
33	27835.0
34	32917.0
35	37999.0
36	43483.5
37	48968.0
38	52250.5
39	55533.0
40	55533.0
41	60344.0
42	65155.0
43	67634.5
44	70114.0
45	72616.0
46	75118.0
47	75118.0
48	76997.5
49	78877.0
50	83892.0
51	88907.0
52	93844.0
53	98781.0
54	98781.0
55	100373.0
56	101965.0
57	97640.5
58	93316.0
59	85517.0
60	77718.0
61	77718.0
62	76137.0
63	74556.0
64	65305.5
65	56055.0
66	47850.5
67	39646.0
68	39646.0
69	34429.0
70	29212.0
71	27083.5
72	24955.0
73	27355.0
74	29755.0
75	29755.0
76	27927.0
77	26099.0
78	19976.0
79	13853.0
80	8820.0
81	3787.0
82	3787.0
83	2646.0
84	1505.0
85	1182.0
86	859.0
87	750.0
88	641.0
89	641.0
90	517.0
91	393.0
92	226.5
93	60.0
94	42.5
95	25.0
96	25.0
97	16.5
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1296883.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.53521083632501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.36898485363133	42.13069226788646
2	9.48472148440785	9.586247998767444
3	2.718103199189888	4.120797547378515
4	1.181434501128077	2.388161664152633
5	0.67011863082436	1.6932293147029238
6	0.4166254621002612	1.2632553340210817
7	0.29523951770491	1.0443993892102776
8	0.2197530498869725	0.8884213366370882
9	0.1660952095189616	0.7554290788749871
>10	1.21604242238913	12.090071339683885
>50	0.1410366101886902	4.871270383198754
>100	0.10533406544307086	10.57654699318443
>500	0.011313088198221403	4.019116360068624
>1k	0.005045025818125761	4.1285137066884605
>5k	1.5287957024623519E-4	0.4438472855444666
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	5745	0.4429852191755155	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	3597	0.2773573252174637	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2257	0.17403266138888396	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2233	0.17218207039493924	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2209	0.17033147940099452	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	2143	0.16524235416764657	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2127	0.1640086268383501	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	1985	0.15305929679084387	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1906	0.14696776810244255	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1898	0.1463509044377943	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1891	0.1458111487312271	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	1751	0.13501603459988293	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	1743	0.1343991709352347	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	1654	0.12753656266602306	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1611	0.1242209204685388	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1591	0.1226787613069182	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	1542	0.11890047136094775	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	1536	0.11843782361246158	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	1530	0.1179751758639754	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	1523	0.1174354201574082	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	1498	0.11550772120538244	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1482	0.11427399387608597	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	1327	0.10232226037352636	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	7.710795808102966E-5	0.0	0.0	0.0	0.0
6	7.710795808102966E-5	0.0	0.0	0.0	0.0
7	7.710795808102966E-5	0.0	0.0	0.0	0.0
8	7.710795808102966E-5	0.0	0.0	0.0	0.0
9	7.710795808102966E-5	0.0	0.0	0.0	0.0
10	7.710795808102966E-5	0.0	0.0	0.0	0.0
11	7.710795808102966E-5	0.0	0.0	7.710795808102966E-5	0.0
12	7.710795808102966E-5	0.0	0.0	1.5421591616205933E-4	0.0
13	7.710795808102966E-5	0.0	0.0	1.5421591616205933E-4	0.0
14	7.710795808102966E-5	0.0	0.0	2.31323874243089E-4	0.0
15	7.710795808102966E-5	0.0	0.0	2.31323874243089E-4	0.0
16	7.710795808102966E-5	0.0	0.0	5.397557065672077E-4	0.0
17	7.710795808102966E-5	0.0	0.0	0.001156619371215445	0.0
18	3.0843183232411866E-4	0.0	0.0	0.0012337273292964746	0.0
19	3.0843183232411866E-4	0.0	0.0	0.0013108352873775044	0.0
20	3.0843183232411866E-4	0.0	0.0	0.0014650512035395637	0.0
21	3.0843183232411866E-4	0.0	0.0	0.001850590993944712	0.0
22	3.0843183232411866E-4	0.0	0.0	0.003084318323241187	0.0
23	3.0843183232411866E-4	0.0	0.0	0.004240937694456632	0.0
24	3.8553979040514835E-4	0.0	0.0	0.005860204814158255	0.0
25	3.8553979040514835E-4	0.0	0.0	0.007171040101535759	0.0
26	3.8553979040514835E-4	0.0	0.0	0.010101142508614887	0.0
27	3.8553979040514835E-4	0.0	0.0	0.019816745226824625	0.0
28	3.8553979040514835E-4	0.0	0.0	0.041329865531431906	0.0
29	3.8553979040514835E-4	0.0	0.0	0.07132486122495245	0.0
30	3.8553979040514835E-4	0.0	0.0	0.10849089702000875	0.0
31	3.8553979040514835E-4	0.0	0.0	0.20672643561524054	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTT	70	1.9233266E-7	23.785713	12
CTATCGG	40	0.0019313198	23.125002	12
TTAGCGA	50	2.7023608E-4	22.2	27
GGTATCA	970	0.0	21.742268	1
GTATACT	70	5.1027564E-6	21.142857	4
TTAGACT	100	1.2902092E-8	20.35	4
GACAGGC	1305	0.0	19.846743	7
TGCATCA	1295	0.0	19.57143	14
GCTGCAT	1340	0.0	19.328358	12
ACAGGCT	1350	0.0	19.322222	8
CGTAGCC	115	3.0504452E-9	19.304348	3
GCATCAG	1320	0.0	19.200758	15
CCAGGAC	1485	0.0	18.686869	3
CAGGACA	1515	0.0	18.683168	4
CTGCATC	1445	0.0	17.795847	13
GGACAGG	1565	0.0	17.495209	6
TTATACG	75	2.0675235E-4	17.266666	35
TATACGA	75	2.0675235E-4	17.266666	36
CGAACGA	130	1.3935278E-8	17.076923	16
GTACGTA	65	0.0015800815	17.076923	13
>>END_MODULE