##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630443.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1740
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.345977011494252	31.0	30.0	31.0	27.0	34.0
2	30.57816091954023	31.0	30.0	31.0	27.0	34.0
3	30.54367816091954	31.0	30.0	33.0	27.0	34.0
4	34.53793103448276	35.0	35.0	37.0	30.0	37.0
5	34.22471264367816	35.0	35.0	37.0	30.0	37.0
6	34.285057471264366	35.0	35.0	37.0	30.0	37.0
7	34.25229885057471	35.0	35.0	37.0	30.0	37.0
8	34.214942528735634	35.0	35.0	37.0	30.0	37.0
9	35.770114942528735	37.0	35.0	39.0	30.0	39.0
10	35.5448275862069	37.0	35.0	39.0	30.0	39.0
11	35.757471264367815	37.0	35.0	39.0	30.0	39.0
12	35.60632183908046	37.0	35.0	39.0	30.0	39.0
13	35.61379310344827	37.0	34.0	39.0	30.0	39.0
14	36.487931034482756	38.0	35.0	40.0	31.0	41.0
15	36.394827586206894	38.0	35.0	40.0	30.0	41.0
16	36.15689655172414	38.0	34.0	40.0	30.0	41.0
17	36.04942528735632	38.0	34.0	40.0	29.0	41.0
18	36.13390804597701	38.0	34.0	40.0	30.0	41.0
19	36.36149425287356	38.0	34.0	40.0	30.0	41.0
20	36.3316091954023	38.0	34.0	40.0	30.0	41.0
21	36.052298850574715	38.0	34.0	40.0	30.0	41.0
22	36.15862068965517	38.0	34.0	40.0	30.0	41.0
23	36.4051724137931	38.0	35.0	40.0	30.0	41.0
24	36.10287356321839	38.0	34.0	40.0	30.0	41.0
25	35.8816091954023	38.0	34.0	40.0	30.0	41.0
26	35.641379310344824	38.0	34.0	40.0	27.0	41.0
27	35.33045977011494	38.0	34.0	40.0	27.0	41.0
28	35.30747126436781	38.0	34.0	40.0	27.0	41.0
29	35.12528735632184	37.0	33.0	40.0	27.0	40.0
30	35.189655172413794	37.0	33.0	40.0	27.0	41.0
31	35.21781609195402	37.0	34.0	40.0	27.0	40.0
32	35.09310344827586	37.0	33.0	40.0	27.0	40.0
33	34.827586206896555	37.0	33.0	40.0	26.0	41.0
34	34.82586206896552	37.0	33.0	40.0	26.0	40.0
35	34.527011494252875	37.0	33.0	39.0	25.0	41.0
36	34.086781609195405	37.0	32.0	39.0	24.0	40.0
37	34.203448275862065	37.0	32.0	39.0	24.0	40.0
38	34.09712643678161	37.0	32.0	39.0	24.0	40.0
39	34.10057471264368	37.0	32.0	39.0	24.0	40.0
40	33.842528735632186	37.0	31.0	39.0	24.0	40.0
41	33.51379310344828	36.0	31.0	39.0	22.0	40.0
42	33.59310344827586	37.0	31.0	39.0	23.0	40.0
43	32.37413793103448	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	0.0
21	3.0
22	4.0
23	10.0
24	7.0
25	12.0
26	20.0
27	35.0
28	40.0
29	45.0
30	83.0
31	84.0
32	129.0
33	129.0
34	151.0
35	187.0
36	221.0
37	235.0
38	245.0
39	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.689655172413794	21.839080459770116	12.988505747126435	24.482758620689655
2	18.275862068965516	22.24137931034483	33.90804597701149	25.57471264367816
3	18.735632183908045	24.655172413793103	30.804597701149426	25.804597701149422
4	14.655172413793101	15.229885057471265	33.62068965517241	36.49425287356322
5	12.35632183908046	36.321839080459775	37.12643678160919	14.195402298850574
6	35.11494252873563	34.08045977011494	16.379310344827587	14.425287356321839
7	28.965517241379313	32.241379310344826	20.517241379310345	18.275862068965516
8	27.52873563218391	34.195402298850574	20.28735632183908	17.98850574712644
9	27.35632183908046	14.195402298850574	20.057471264367816	38.39080459770115
10	16.551724137931036	27.873563218390807	32.64367816091954	22.93103448275862
11	35.91954022988506	22.24137931034483	19.597701149425287	22.24137931034483
12	18.448275862068968	25.459770114942533	29.367816091954026	26.72413793103448
13	28.50574712643678	20.17241379310345	27.413793103448274	23.908045977011493
14	21.03448275862069	20.229885057471265	27.47126436781609	31.264367816091955
15	26.72413793103448	27.816091954022987	21.89655172413793	23.563218390804597
16	24.942528735632184	26.03448275862069	24.25287356321839	24.770114942528735
17	22.24137931034483	27.068965517241377	27.241379310344826	23.448275862068964
18	21.436781609195403	24.71264367816092	29.540229885057474	24.310344827586206
19	24.022988505747126	25.114942528735636	27.413793103448274	23.448275862068964
20	23.678160919540232	25.689655172413794	25.402298850574713	25.229885057471265
21	24.42528735632184	26.551724137931032	26.89655172413793	22.126436781609197
22	24.770114942528735	25.57471264367816	26.551724137931032	23.103448275862068
23	22.64367816091954	27.47126436781609	26.95402298850575	22.93103448275862
24	24.71264367816092	26.666666666666668	26.436781609195403	22.18390804597701
25	24.655172413793103	25.287356321839084	25.804597701149422	24.25287356321839
26	24.54022988505747	24.82758620689655	28.045977011494255	22.586206896551726
27	23.39080459770115	26.14942528735632	26.379310344827587	24.080459770114942
28	23.448275862068964	26.091954022988507	26.72413793103448	23.735632183908045
29	22.356321839080458	26.95402298850575	26.72413793103448	23.96551724137931
30	22.528735632183906	26.89655172413793	27.873563218390807	22.701149425287355
31	24.885057471264368	25.344827586206897	26.666666666666668	23.103448275862068
32	23.333333333333332	26.551724137931032	26.14942528735632	23.96551724137931
33	24.25287356321839	24.367816091954023	28.333333333333332	23.045977011494255
34	23.103448275862068	26.14942528735632	27.52873563218391	23.218390804597703
35	24.195402298850574	26.551724137931032	27.47126436781609	21.7816091954023
36	23.79310344827586	25.229885057471265	27.70114942528736	23.275862068965516
37	23.678160919540232	27.183908045977013	25.114942528735636	24.022988505747126
38	23.620689655172413	27.298850574712645	25.689655172413794	23.39080459770115
39	23.850574712643677	25.17241379310345	27.64367816091954	23.333333333333332
40	23.448275862068964	24.655172413793103	27.183908045977013	24.71264367816092
41	22.18390804597701	25.287356321839084	30.05747126436782	22.47126436781609
42	23.045977011494255	26.379310344827587	28.045977011494255	22.528735632183906
43	22.988505747126435	23.908045977011493	26.7816091954023	26.321839080459768
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.5
4	2.0
5	2.0
6	2.5
7	3.0
8	2.0
9	1.0
10	0.5
11	0.0
12	0.0
13	5.0
14	10.0
15	13.5
16	17.0
17	13.0
18	9.0
19	9.0
20	13.5
21	18.0
22	18.0
23	18.0
24	23.0
25	28.0
26	28.0
27	29.5
28	31.0
29	42.0
30	53.0
31	58.0
32	63.0
33	63.0
34	71.5
35	80.0
36	74.5
37	69.0
38	82.5
39	96.0
40	96.0
41	95.5
42	95.0
43	97.5
44	100.0
45	108.5
46	117.0
47	117.0
48	123.5
49	130.0
50	135.5
51	141.0
52	129.5
53	118.0
54	118.0
55	117.0
56	116.0
57	100.5
58	85.0
59	87.0
60	89.0
61	89.0
62	80.5
63	72.0
64	59.0
65	46.0
66	45.5
67	45.0
68	45.0
69	37.0
70	29.0
71	28.0
72	27.0
73	19.0
74	11.0
75	11.0
76	7.5
77	4.0
78	7.5
79	11.0
80	7.0
81	3.0
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1740.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.12643678160919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.86982248520711	95.05747126436782
2	1.7751479289940828	3.4482758620689653
3	0.2366863905325444	0.6896551724137931
4	0.0	0.0
5	0.0	0.0
6	0.0591715976331361	0.3448275862068966
7	0.0	0.0
8	0.0591715976331361	0.45977011494252873
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.45977011494252873	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.3448275862068966	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	3	0.1724137931034483	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.1724137931034483	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	3	0.1724137931034483	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	3	0.1724137931034483	No Hit
GGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2	0.11494252873563218	No Hit
AATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCTGATCAG	2	0.11494252873563218	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.11494252873563218	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	2	0.11494252873563218	No Hit
CTTCTATTCTCCTATTATTATAATGCAGATATGTCAGATCGGA	2	0.11494252873563218	No Hit
TAACTATATCACTATTCAAGATCATCTTCACAACATCACCTGC	2	0.11494252873563218	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.11494252873563218	No Hit
CGTCACGATGAATTTGAGAGACATGCTGAAGGGACCTTTACCA	2	0.11494252873563218	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	2	0.11494252873563218	No Hit
ATTACAAATAATCCAGCCACAAAGTAAATGCTTTTCATTTCTG	2	0.11494252873563218	No Hit
GTTGTGAGCCCATGCAGCTCTCCAGACTCACTGGTTTTCCCAG	2	0.11494252873563218	No Hit
GCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCC	2	0.11494252873563218	No Hit
ACATTGCTTTGCATGCCAATAAATAAATTTTCTTTTAGTGTTG	2	0.11494252873563218	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2	0.11494252873563218	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	2	0.11494252873563218	No Hit
TTCACCAGCCAAGCAATGAATTCCTTGGCAGCTTGGCCTTCCA	2	0.11494252873563218	No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC	2	0.11494252873563218	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG	2	0.11494252873563218	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2	0.11494252873563218	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	2	0.11494252873563218	No Hit
GCTCTGTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAA	2	0.11494252873563218	No Hit
TAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCA	2	0.11494252873563218	No Hit
GAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCTCTGTGTCC	2	0.11494252873563218	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	2	0.11494252873563218	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2	0.11494252873563218	No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA	2	0.11494252873563218	No Hit
CGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT	2	0.11494252873563218	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	2	0.11494252873563218	No Hit
TAGTAAGGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAG	2	0.11494252873563218	No Hit
CTACAGGAGGAAACGCGAGCGTGGTGGGACTGGCTCCAGGCAC	2	0.11494252873563218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.05747126436781609	0.0
28	0.0	0.0	0.0	0.05747126436781609	0.0
29	0.0	0.0	0.0	0.05747126436781609	0.0
30	0.0	0.0	0.0	0.05747126436781609	0.0
31	0.0	0.0	0.0	0.1724137931034483	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE