FastQCFastQC Report
Fri 10 Feb 2017
ERR1630442.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630442.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939489
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACA1700.020.6764724
AATACGA909.4736606E-820.5555575
GCCGTAT450.003825295320.5555578
CTAATAC1450.020.4137943
GTAATAC555.1421346E-420.1818183
GACGGAC1153.0486262E-919.3043467
CAATACG1254.129106E-1019.2400024
GGTATCA5400.018.8425921
ATTAGAC500.00703394918.53
AAGACGG1205.169568E-918.55
GTTCTAG1850.018.01
CGAAAAC1258.572897E-917.76000223
TAGAACT2850.017.5263184
GTCCTAA852.7222579E-517.4117641
GTATTAC752.0667595E-417.2666661
TCCGAAA1201.0405711E-716.95833421
TGTAATA1107.803865E-716.8181822
TCTAGCG803.381742E-416.187528
CCGTATG803.381742E-416.18759
GTCTAGA1951.8189894E-1216.1282061