##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630442.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	939489
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.791302505936738	31.0	31.0	34.0	30.0	34.0
2	32.00018627147311	33.0	31.0	34.0	30.0	34.0
3	32.13487331943216	34.0	31.0	34.0	30.0	34.0
4	35.78817740282217	37.0	35.0	37.0	35.0	37.0
5	35.7193453036704	37.0	35.0	37.0	35.0	37.0
6	35.77021018873025	37.0	35.0	37.0	35.0	37.0
7	35.74523065198209	37.0	35.0	37.0	33.0	37.0
8	35.73607567518087	37.0	35.0	37.0	33.0	37.0
9	37.48930003438039	39.0	37.0	39.0	35.0	39.0
10	37.370301302090816	39.0	37.0	39.0	34.0	39.0
11	37.452945164871544	39.0	37.0	39.0	35.0	39.0
12	37.35478648499344	39.0	37.0	39.0	34.0	39.0
13	37.36797769851483	39.0	37.0	39.0	34.0	39.0
14	38.58633469896933	40.0	38.0	41.0	34.0	41.0
15	38.59645935183914	40.0	38.0	41.0	34.0	41.0
16	38.549926609039595	40.0	38.0	41.0	34.0	41.0
17	38.513714370258725	40.0	38.0	41.0	34.0	41.0
18	38.46775960123003	40.0	38.0	41.0	34.0	41.0
19	38.4942250521294	40.0	38.0	41.0	34.0	41.0
20	38.46961912273587	40.0	38.0	41.0	34.0	41.0
21	38.40827620121151	40.0	38.0	41.0	34.0	41.0
22	38.364217143574855	40.0	38.0	41.0	34.0	41.0
23	38.31006536532094	40.0	38.0	41.0	34.0	41.0
24	38.26296316401788	40.0	38.0	41.0	34.0	41.0
25	38.24810402250585	40.0	38.0	41.0	34.0	41.0
26	38.09104949605584	40.0	38.0	41.0	33.0	41.0
27	37.959847321256554	40.0	37.0	41.0	33.0	41.0
28	37.884650059766535	40.0	37.0	41.0	33.0	41.0
29	37.82798521323826	40.0	37.0	41.0	33.0	41.0
30	37.76852523020493	40.0	37.0	41.0	33.0	41.0
31	37.7212505947382	40.0	37.0	41.0	32.0	41.0
32	37.63233204433474	40.0	37.0	41.0	32.0	41.0
33	37.534620415992094	40.0	37.0	41.0	32.0	41.0
34	37.46940624105232	40.0	37.0	41.0	31.0	41.0
35	37.361430522337145	40.0	37.0	41.0	31.0	41.0
36	37.277824434346755	39.0	36.0	41.0	31.0	41.0
37	37.21247082190425	39.0	36.0	41.0	31.0	41.0
38	37.109606392411195	39.0	36.0	41.0	31.0	41.0
39	37.03593975022592	39.0	36.0	41.0	31.0	41.0
40	36.94033565055046	39.0	36.0	41.0	30.0	41.0
41	36.82515708007225	39.0	35.0	41.0	30.0	41.0
42	36.79126844486737	39.0	35.0	41.0	30.0	41.0
43	35.482794370130996	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	2.0
13	2.0
14	0.0
15	3.0
16	5.0
17	9.0
18	22.0
19	70.0
20	121.0
21	279.0
22	547.0
23	928.0
24	1590.0
25	2611.0
26	3884.0
27	5766.0
28	8286.0
29	11105.0
30	15146.0
31	19932.0
32	25304.0
33	33099.0
34	43529.0
35	57965.0
36	80389.0
37	120217.0
38	221028.0
39	287648.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1172690686107	18.40010899542198	11.563200846417573	26.919421089549743
2	19.393840694249747	20.400983939141383	32.85945870574323	27.345716660865644
3	20.157660174839727	21.508713779512053	29.285601002246963	29.048025043401253
4	14.799747522323306	14.920344996056368	34.069797517586686	36.210109964033634
5	15.57027277594522	35.456295922570675	33.607205619224914	15.366225682259186
6	36.32911082514005	34.451813698723456	14.903740224739192	14.315335251397302
7	30.777475840589936	29.663998194763323	20.11455163392014	19.443974330726597
8	28.808958912770667	32.20750854986061	19.150623370789866	19.83290916657885
9	27.176475722440603	13.309575737448762	18.48111047601409	41.032838064096545
10	17.122925334942718	26.047031950347478	32.52704395687443	24.302998757835375
11	38.1141237417362	20.55819706244565	20.27889629362345	21.048782902194706
12	22.434749102969807	24.425618607562196	28.57925957621643	24.560372713251567
13	31.733846803954062	18.516661717167523	23.610388200394045	26.139103278484367
14	23.695221551290118	19.321567362683332	23.801236629699762	33.18197445632679
15	26.322394408023936	26.659173231405582	21.012167252623502	26.00626510794698
16	26.45576478277021	25.208703880513767	22.375780876625484	25.959750460090536
17	24.88512372151244	25.23063069391978	24.132480529309017	25.751765055258762
18	25.408067577161624	23.999855240455183	24.99433202517539	25.5977451572078
19	25.891628321353416	24.222316599768597	24.41210062065655	25.47395445822144
20	26.047457713714582	24.13514155035344	24.376549379503114	25.44085135642887
21	26.59019956593425	23.94216430421218	24.222742363135705	25.244893766717862
22	26.35837141254448	24.02721053679181	24.25371664809274	25.360701402570974
23	25.84702960864896	24.010073561265752	24.308533681607766	25.834363148477525
24	25.618075357987163	24.38634193694657	24.02582680584871	25.969755899217557
25	26.217550178873832	24.001345412240056	24.106296082231935	25.67480832665417
26	26.25789125790722	23.999535917929855	24.02486883827272	25.7177039858902
27	26.104829327432256	23.93907751980066	24.23434441488937	25.72174873787772
28	25.67480832665417	23.852008911227273	24.55962762735913	25.913555134759424
29	25.10694643577519	24.333015075216423	24.764206925254047	25.79583156375434
30	25.059260938659207	24.807209025331854	24.538552340687332	25.594977695321607
31	25.874597786669135	24.62806908862158	23.877554713253694	25.61977841145559
32	25.57741495642844	24.467982062589343	24.064464831413673	25.890138149568543
33	25.104178973888995	24.128648659005055	24.81785310950953	25.949319257596414
34	26.011693590877595	23.750996552381135	24.50214957279968	25.73516028394159
35	25.452772730707864	23.990275564695278	25.16836280148038	25.38858890311648
36	25.502480603817606	24.28383940631556	24.518435021591525	25.695244968275304
37	25.883751699061936	23.618584145210857	24.931744810210656	25.56591934551655
38	24.976024200389787	23.806771553472153	25.414666909351784	25.802537336786273
39	25.258305312781737	23.787718642794115	25.386885849648056	25.5670901947761
40	24.66809084512964	23.542798265865805	26.041603467416863	25.747507421587695
41	24.476497329931483	23.424329608968282	26.34346969469573	25.755703366404504
42	24.6975749583018	23.127040337885806	26.566889021585137	25.608495682227257
43	24.066700089090983	22.718413946304853	26.65789594130426	26.556990023299896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	18.0
2	29.0
3	78.5
4	128.0
5	128.0
6	162.0
7	196.0
8	180.5
9	165.0
10	250.0
11	335.0
12	335.0
13	607.5
14	880.0
15	1434.0
16	1988.0
17	1991.5
18	1995.0
19	1995.0
20	2779.5
21	3564.0
22	4037.5
23	4511.0
24	5695.5
25	6880.0
26	6880.0
27	8137.0
28	9394.0
29	11451.5
30	13509.0
31	16328.5
32	19148.0
33	19148.0
34	22752.5
35	26357.0
36	30503.5
37	34650.0
38	37706.0
39	40762.0
40	40762.0
41	45076.5
42	49391.0
43	53014.5
44	56638.0
45	59765.5
46	62893.0
47	62893.0
48	65904.5
49	68916.0
50	71401.0
51	73886.0
52	75229.5
53	76573.0
54	76573.0
55	74468.0
56	72363.0
57	69629.0
58	66895.0
59	64767.5
60	62640.0
61	62640.0
62	59241.0
63	55842.0
64	49789.5
65	43737.0
66	37931.5
67	32126.0
68	32126.0
69	26560.5
70	20995.0
71	17788.5
72	14582.0
73	11169.0
74	7756.0
75	7756.0
76	5990.0
77	4224.0
78	3352.0
79	2480.0
80	2032.0
81	1584.0
82	1584.0
83	1125.0
84	666.0
85	532.5
86	399.0
87	309.5
88	220.0
89	220.0
90	178.0
91	136.0
92	85.0
93	34.0
94	23.0
95	12.0
96	12.0
97	7.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	939489.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.23810355298108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.80017606056055	51.93001962907633
2	8.603919727104403	10.537754544199123
3	2.451452390816597	4.503668860920895
4	1.1153691005122375	2.7321235390785503
5	0.660136456631452	2.0212752345147433
6	0.4288385994538982	1.5756757536523915
7	0.324426699510438	1.3907093073980454
8	0.22469132837318398	1.1007736667499133
9	0.17913640987846413	0.9872976616421985
>10	1.0500093969986124	12.37425554573107
>50	0.10760980799909875	4.567157172236219
>100	0.052664548905437994	5.622419928192854
>500	0.0015694732556482997	0.656869156607618
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	1.0644084177675311E-4	0.0	0.0	0.0
12	0.0	1.0644084177675311E-4	0.0	0.0	0.0
13	0.0	1.0644084177675311E-4	0.0	0.0	0.0
14	0.0	1.0644084177675311E-4	0.0	1.0644084177675311E-4	0.0
15	0.0	1.0644084177675311E-4	0.0	2.1288168355350622E-4	0.0
16	0.0	1.0644084177675311E-4	0.0	3.193225253302593E-4	0.0
17	0.0	1.0644084177675311E-4	0.0	5.322042088837655E-4	0.0
18	0.0	1.0644084177675311E-4	0.0	5.322042088837655E-4	0.0
19	0.0	1.0644084177675311E-4	0.0	7.450858924372718E-4	0.0
20	0.0	1.0644084177675311E-4	0.0	7.450858924372718E-4	1.0644084177675311E-4
21	0.0	1.0644084177675311E-4	0.0	8.515267342140249E-4	1.0644084177675311E-4
22	0.0	1.0644084177675311E-4	0.0	0.0014901717848745436	1.0644084177675311E-4
23	0.0	1.0644084177675311E-4	0.0	0.0023416985190885683	1.0644084177675311E-4
24	0.0	1.0644084177675311E-4	0.0	0.0036189886204096057	1.0644084177675311E-4
25	0.0	1.0644084177675311E-4	0.0	0.004576956196400384	1.0644084177675311E-4
26	0.0	1.0644084177675311E-4	0.0	0.006280009664828433	1.0644084177675311E-4
27	0.0	1.0644084177675311E-4	0.0	0.012985782696763879	1.0644084177675311E-4
28	0.0	1.0644084177675311E-4	0.0	0.030761403273481647	1.0644084177675311E-4
29	0.0	1.0644084177675311E-4	0.0	0.05758449540122343	1.0644084177675311E-4
30	0.0	1.0644084177675311E-4	0.0	0.09994795042837117	1.0644084177675311E-4
31	0.0	1.0644084177675311E-4	0.0	0.2154362637561483	1.0644084177675311E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACA	170	0.0	20.676472	4
AATACGA	90	9.4736606E-8	20.555557	5
GCCGTAT	45	0.0038252953	20.555557	8
CTAATAC	145	0.0	20.413794	3
GTAATAC	55	5.1421346E-4	20.181818	3
GACGGAC	115	3.0486262E-9	19.304346	7
CAATACG	125	4.129106E-10	19.240002	4
GGTATCA	540	0.0	18.842592	1
ATTAGAC	50	0.007033949	18.5	3
AAGACGG	120	5.169568E-9	18.5	5
GTTCTAG	185	0.0	18.0	1
CGAAAAC	125	8.572897E-9	17.760002	23
TAGAACT	285	0.0	17.526318	4
GTCCTAA	85	2.7222579E-5	17.411764	1
GTATTAC	75	2.0667595E-4	17.266666	1
TCCGAAA	120	1.0405711E-7	16.958334	21
TGTAATA	110	7.803865E-7	16.818182	2
TCTAGCG	80	3.381742E-4	16.1875	28
CCGTATG	80	3.381742E-4	16.1875	9
GTCTAGA	195	1.8189894E-12	16.128206	1
>>END_MODULE